Rc210241

The plasmid pCMV-3 × HA1-HBx (HA-HBx), which encodes HBx (genotype D) downstream of influenza virus haemagglutinin (HA) tag (YPYDVPDYA), was previously described [17 (link)]. The 1.2-mer wild-type (WT) HBV replicon, which contains 1.2 units of the HBV genome (1.2-mer WT; genotype D), and its HBx-null counterpart (1.2-mer HBx-null) were previously described [28 (link)]. The HBV core promoter/enhancer reporter, pHBV-luc, was previously described [28 (link)]. The plasmid pSiah-1-Myc WT encoding Myc-tagged Siah-1 was previously described [29 (link)]. The plasmid RC210241 (Cat No. 003049) encoding human sodium taurocholate cotransporting polypeptide (NTCP) was purchased from OriGene (Rockville, MD, USA). Scrambled (SC) short hairpin RNA (shRNA) (Cat No. sc-37007) and p53 shRNA (Cat. sc-29435) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Siah-1 shRNA (Cat No. SHCLND-NM003031) was obtained from Sigma-Aldrich (St. Louis, MO, USA). The plasmid pCH110 (Cat No. 27-4508-01) encoding the Escherichia coli β-galactosidase (β-Gal) gene was purchased from Addgene (Watertown, MA, USA). pCMV p53-WT and pHA-Ub were kindly provided by Dr. C.-W. Lee (Sungkyunkwan University, Suwon, Republic of Korea) and Dr. Y. Xiong (UNC-Chapel Hill, NC, USA), respectively.

The plasmids pCMV-3×HA1-Core (HA-Core) (53 (link)) and pCMV-3×HA1-HBx (HA-HBx) (54 (link)) encode the full-length HCV core protein (genotype 1b) and HBx (genotype D), respectively, downstream of the three copies of influenza virus hemagglutinin (HA). The plasmid pCI-neo-core K, which encodes the core region of the HCV-K isolate (genotype 1b) under the control of the human cytomegalovirus immediate early promoter, was described previously (55 (link)). The construction of a DNMT1 short hairpin RNA (shRNA) plasmid using the SirenCircle RNA interference (RNAi) system (Allele Biotechnology) was described previously (56 (link)). The 1.2-mer WT HBV replicon containing 1.2 U of the HBV genome (genotype D) and its HBx-null counterpart (57 (link)) were kindly provided by W.-S. Ryu (Yonsei University, Republic of Korea). The E6AP expression plasmid pCMVT N-HA-hE6AP (catalog no. 37601), encoding full-length human HA-tagged E6AP, was purchased from Addgene. The plasmids RC210241 (catalog no. 003049), encoding human NTCP, and pCH110 (catalog no. 27-4508-01), carrying the Escherichia coli β-galactosidase (β-Gal) gene, were obtained from OriGene and Amersham, respectively. The plasmid pHA-Ub was a gift from Y. Xiong (University of North Carolina at Chapel Hill, Chapel Hill, NC, USA).