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Ssvi first strand synthesis kit

Manufactured by Thermo Fisher Scientific
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The SSVI first strand synthesis kit is a laboratory tool designed for the reverse transcription of RNA into complementary DNA (cDNA). The kit provides the necessary components, including enzymes and buffers, to efficiently convert RNA into single-stranded cDNA, which can then be used in various downstream applications, such as gene expression analysis and cDNA library construction.

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Lab products found in correlation

Dnase 1 amp grade, by Thermo Fisher Scientific (2 mentions) Rneasy plant mini kit, by Qiagen (2 mentions)

2 protocols using ssvi first strand synthesis kit

1

Plant RNA Extraction and cDNA Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seven-day-old light-grown seedlings were flash-frozen in liquid nitrogen and RNA was extracted using the Qiagen Plant RNeasy Mini kit according to the manufacturer’s instructions. RNA integrity was verified via gel electrophoresis. DNA was removed by treatment with DnaseI (Amp-grade, Invitrogen) and cDNA was generated using the SSVI first strand synthesis kit (Invitrogen) with the oligo dT20 primer provided, according to the manufacturer’s instructions. RT-PCR was performed using primers indicated in Table S2.
McFarlane H.E., Mutwil-Anderwald D., Verbančič J., Picard K.L., Gookin T.E., Froehlich A., Chakravorty D., Trindade L.M., Alonso J.M., Assmann S.M, & Persson S. (2021). A G protein-coupled receptor-like module regulates cellulose synthase secretion from the endomembrane system in Arabidopsis. Developmental cell, 56(10), 1484-1497.e7.
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2

RNA Extraction and RT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seven-day-old light-grown seedlings were flash-frozen in liquid nitrogen and RNA was extracted using the Qiagen Plant RNeasy Mini kit according to the manufacturer's instructions. RNA integrity was verified via gel electrophoresis. DNA was removed by treatment with DnaseI (Amp-grade, Invitrogen) and cDNA was generated using the SSVI first strand synthesis kit (Invitrogen) with the oligo dT 20 primer provided according to the manufacturer's instructions. RT-PCR was performed using primers indicated in Supplemental Table 3.
McFarlane H.E., Mutwil‐Anderwald D., Verbančič J., Picard K.L., Gookin T.E., Froehlich A., Trindade L.M., Alonso J.M., Assmann S.M., & Persson S. (2020). A G protein-coupled receptor-like module regulates Cellulose Synthase secretion from the endomembrane system in Arabidopsis.
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