Normal, wild-type (WT) C57BL/6J mice were purchased from the Institute of Zoology (Chinese Academy of Sciences, Beijing, China). miR-21flox/+ (miR-21fl/+) mice and Mx1-Cre mice were obtained from Shanghai Model Organisms Center (China). miR- 21fl/fl;Mx1-Cre mice were generated by crossing miR-21fl/fl mice with Mx1-Cre mice. Unless otherwise stated, miR-21 deletion was induced by intraperitoneally injecting 4- to 6-week old miR- 21fl/fl;Mx1-Cre+mice with 250 g of polyinosinic:polycytidylic acid (pIpC) (Sigma, St. Louis, MO, USA) every other day for a total of seven doses. Four weeks after pIpC treatment, these mice were used in subsequent experiments. Identically treated miR- 21fl/fl;Mx1-Cre- littermates served as controls. Congenic C57Bl/6 SJL CD45.1+ mice were kindly provided by Prof. Jinyong Wang (Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Science, Guangzhou, China). All animal experiments were approved by the Animal Care Committee of The Third Military Medical University (Chongqing, China).
Mx1 cre mice
Manufactured by Shanghai Model Organisms
Sourced in China
Mx1-cre mice are a genetically modified mouse strain that expresses the Cre recombinase enzyme under the control of the Mx1 promoter. The Mx1 promoter is inducible and can be activated by interferon or synthetic double-stranded RNA, leading to Cre expression in specific cell types. This mouse model is a useful tool for conditional gene manipulation studies.
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3 protocols using mx1 cre mice
1
Conditional Deletion of miR-21 in Mice
2
Conditional Deletion of Myh9 in Mice
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Floxed Myh9 (Myh9fl/fl) mice were described previously [19 (link)] and, as a generous gift from Prof. Luo jincai, Mx1-cre mice were purchased from Shanghai Model Organisms Center, Inc. To conditionally delete the Myh9 floxed allele, we mated Myh9fl/fl mice with Mx1-cre mice to generate Myh9fl/fl:Mx1-cre mice. Cre expression was induced by intraperitoneally injecting three doses of 5 mg/kg body weight polyinosinic-polycytidylic acid (poly I:C) at 5–6 weeks after birth. All mice subjected to this knockout model and other experiments were on a C57BL/6 background with littermate controls, unless stated otherwise. Genotyping primer sequences are listed in the Supplementary Table S1 . All animal studies were performed in accordance with the guidelines approved by the Institutional Ethics Review Committee of Institute of Blood Transfusion (IERC-IBT).
3
Conditional Dlk1 KO Mice for Immune Response
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Dlk1 conditional KO mice was generated by Jennifer Schmidt, University of Illinois at Chicago, and we purchased from Jackson lab. ptprc mutant mice and Mx1-cre mice were purchased from Shanghai Model organisms. All mice were housed under specific pathogen-free conditions in the Laboratory Animal Center of Zhejiang University (ZJU). The genotype identification of mice offspring was performed 3–4 weeks after birth. The mice offspring were intraperitoneally injected every other day with 2 mg/kg b.w. poly I:C for 7 times after genotype identification. The control mice were treated with the same conditions with the Dlk1 knockout mice. The flow cytometric analysis was carried out at least 1 month after injection of poly I:C.
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