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2 protocols using medroxyprogesterone acetate

1

Signaling Pathway Protein Analysis

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Antibodies for YAP1(ab76252), PGR(ab23085), and TBP (ab818) were purchased from Abcam (Cambridge, UK); TAZ (#83669), β-actin(#4970), MST1(#3682), parp(#2772), cleaved caspase 3(#9664), cleaved caspase 7(#8438), cleaved caspase 9(#20750), bax(#2772), and CyclinD1(#2978), Akt(#4691), p-Akt(#13038), anti-flag(#14793), anti-rabbit IgG(#7074), anti-mouse IgG(#7076) were from Cell Signaling Technology (Danvers, MA,USA). Antibody for LATS1(#sc-398560) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for p-MST1(AP0906), p-LATS1(AP0904), p-YAP(AP0489) were from ABcolonal (Wuhan, China). Medroxyprogesterone acetate, abbreviated to MPA (#ab142633) was purchased from Abcam and Verteporfin (CL318952) was purchased from MedChem Express (Monmouth Junction, NJ, USA).
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2

Investigating Lipogenesis and Cell Cycle Regulation

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For this study, we purchased the following antibodies against SREBP1 (Abcam, ab191857), FASN(Abcam,ab96866), ACLY(Abcam,ab40793), SCD1(Abcam,ab236868), PGR (Abcam, ab32085), Cyclin D1 (Cell Signaling Technologies, #2978), CDK4 (Cell Signaling Technologies, #12790), PARP (Cell Signaling Technologies, #9542), cleaved-PARP (Cell Signaling Technologies, #5625), Bax (Cell Signaling Technologies, #2772), Bcl2 (Cell Signaling Technologies, #4223), NF-κB(Cell Signaling Technologies,#8242), p-NF-κB (Cell Signaling Technologies, #3033), β-actin (Cell Signaling Technologies, #4970), ki67 (Abcam, ab92742), cleaved-casepase3 (Cell Signaling Technologies, #9664), mouse IgG (Cell Signaling Technologies, #7076), and rabbit IgG (Cell Signaling Technologies, #7074). Medroxyprogesterone acetate and Fatostatin were obtained from Abcam and MedChem Express, respectively, and were both diluted in DMSO.
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