C57 mice (weight 20–25 g, male) were obtained from the Changzhou CAVENS Laboratory Animal (Jiangsu, China). HK-2 cells were acquired from the BeNa Culture Collection (Beijing, China). The following materials were also used: anti-FNDC3B antibody (Atlas Antibodies AB, Sweden); anti-TGF-β1 antibody (Boster, Wuhan, China); anti-GAPDH antibody (Service Bio, Wuhan, China); anti-caspase3 antibody (Boster, Wuhan, China); TRIzol (Invitrogen, USA); fetal bovine serum (Gibco, USA); TUNEL Kit (Roche, Shanghai, China); Hoechst (Service Bio, Wuhan, China); Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA); Bio-Rad S1000 with Bestar SYBR Green RT-PCR Master Mix (DBI Bioscience, Shanghai, China); and AAV GPAAV-CMV-MCS-EF1-ZsGreen1-WPRE (Genomeditech Co., LTD, Shanghai, China).
S1000 with bestar sybr green rt pcr master mix
Manufactured by DBI Bioscience
Sourced in United States
The S1000 with Bestar SYBR Green RT-PCR Master Mix is a lab equipment product that enables real-time reverse transcription polymerase chain reaction (RT-PCR) analysis. It includes the S1000 thermal cycler and the Bestar SYBR Green RT-PCR Master Mix, a premixed solution containing all the necessary reagents for the RT-PCR reaction.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hoechst, by Wuhan Servicebio Technology (1 mentions)
Anti caspase3 antibody, by Boster Bio (1 mentions)
Anti gapdh antibody, by Wuhan Servicebio Technology (1 mentions)
Anti tgf β1 antibody, by Boster Bio (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Tunel kit, by Roche (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
2 protocols using s1000 with bestar sybr green rt pcr master mix
1
Investigating FNDC3B in Kidney Cell Apoptosis
2
Validating RNA-seq data by qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In this study, qPCR was performed on GHR and GH, to elucidate the validity of the RNA-seq data, normalized with the reference gene GAPDH. Primers (Tables S-4， S-5，S-6) The same RNA samples for RNA-seq were used for qPCR. 1 μg of RNA was reverse-transcribed using the PrimeScriptTM RT Reagent Kit (Takara) following the manufacturer's instructions. qPCR was performed on the Bio-Rad S1000 with
Bestar SYBR Green RT-PCR Master Mix (DBI Bioscience).
Bestar SYBR Green RT-PCR Master Mix (DBI Bioscience).
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