Six rats from each group were sacrificed either 6 h or 24 h after the operation by isoflurane overdose (6–8 vol%) via inhalation. Prior to death, 3 mL of blood samples were harvested from the inferior vena cava, centrifuged with 3000 rpm for 30 min at 4°C, and immediately stored at−80°C. To evaluate the effects of etomidate on organ injury under septic conditions, serum alanine aminotransferase (ALT) and creatinine levels were assessed with an automatic biochemistry analyzer (Hitachi 7180; Hitachi High-Technologies Corp., Tokyo, Japan) and commercially available clinical assay kits. To investigate the effects of etomidate on early-onset pro-inflammatory cytokines, TNF-α, and IL-6 levels were measured with a rat TNF-α Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, USA) and a rat IL-6 Quantikine ELISA kit (R&D Systems), respectively. Serum HMGB1 level was analyzed to reveal the effect of etomidate on the release of HMGB1 using a rat HMGB1 ELISA kit (IBL-Hamburg, Hamburg, Germany).
Rat tnf α quantikine enzyme linked immunosorbent assay elisa kit
Manufactured by R&D Systems
Sourced in United States
The Rat TNF-α Quantikine ELISA kit is a solid-phase enzyme-linked immunosorbent assay designed to measure rat tumor necrosis factor-alpha (TNF-α) levels in cell culture supernates, serum, and plasma. The kit utilizes a quantitative sandwich enzyme immunoassay technique.
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Lab products found in correlation
3 protocols using rat tnf α quantikine enzyme linked immunosorbent assay elisa kit
1
Etomidate's Effects on Organ Injury and Inflammation in Sepsis
2
Rat Plasma Cytokine Quantification
3
Plasma TNF-α and IL-6 Quantification
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Plasma tumor necrosis factor alpha (TNF-α) was measured using the Rat TNF-α Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions. Plasma interleukin 6 (IL-6) was measured using the Rat IL-6 Quantikine ELISA assay kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.
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