After dewaxing and rehydration, the sections were incubated with 3% BSA/0.3% Triton X-100 for 30 min at room temperature. After draining this solution, the slides were incubated at room temperature with rabbit anti-myeloperoxidase (MPO, 1 : 100, Santa Cruz Biotechnologies, Santa Cruz, CA, USA), mouse anti-α smooth muscle actin (α-SMA) (1 : 1000, Sigma-Aldrich, St. Louis, MO, USA), rabbit anti-TM (1 : 100, Santa Cruz), or rabbit anti-CD31 (1 : 200, Santa Cruz) overnight. After rinsing with PBS, the sections were incubated with Alexa-488- or Alexa-594-conjugated secondary antibody (Life Science). After rinsing with PBS, the slides were incubated with DAPI.
Rabbit anti
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Rabbit anti-TM is a primary antibody designed for the detection and study of Toll-like receptor (TM) proteins in various experimental applications. This antibody is raised in rabbits and specifically recognizes the TM protein target.
Automatically generated - may contain errors
2 protocols using rabbit anti
1
Immunohistochemical Analysis of Vascular Markers
2
Signaling Pathway Analysis in UC
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Next, to determine the signal pathway involved in the inhibition of the PCS in UC, MVECs were used to test the expression of endothelial protein C receptor (EPCR), TM, β-arrestin1, pP38 mitogen-activated protein kinase (MAPK), pJNK MAPK, and pERK MAPK with western blot, using their respective antibodies. Primary antibodies goat anti-EPCR, rabbit anti-TM and goat anti-pJNK MAPK (all from Santa Cruz Biotechnology, Santa Cruz, USA) were used here at a concentration of 1:500; rabbit anti-β-arrestin1, rabbit anti-pP38 MAPK, and rabbit anti-pERK MAPK (all from Abcam) were used here at a concentration of 1:500; and the β-actin antibody (Abcam) was used at a concentration of 1:1,000.
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