Six leaves and twelve siliques per treatment were used to determine areas according to the aforementioned approach. Leaves and silique walls were oven dried to constant weight at 60 °C, and dry mass per area (MA) was calculated by dividing the weight of the dry matter by the area. The samples were then milled, and subsamples of 0.15 g were digested with H2SO4-H2O2 [48 (link)], before K concentration determination using a flame 321 photometer (M-410, Cole-Parmer, Chicago, IL, USA). Thereafter, another three leaves and six siliques (with seeds and septa removed) were cut into small segments (approximately 5 mm). After extraction with 80% (v/v) alcohol for 24 h, chlorophyll concentration was determined using a UV–vis spectrophotometer (UV2102, Unico, China) after extracting with 80% (v/v) alcohol for 24 h [49 (link)].
M 410
Manufactured by Cole-Parmer
Sourced in United States
The M-410 is a versatile laboratory instrument designed for precision magnetic stirring. It features a powerful magnetic field that can effectively mix solutions in a variety of laboratory vessels. The M-410 operates at fixed speeds and is suitable for a range of common laboratory applications.
Automatically generated - may contain errors
4 protocols using m 410
1
Leaf and Silique Biomass and Chlorophyll Analysis
2
Root Morphology and Nutrient Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 28 days of treatment, rootstocks were collected to analyse dry weight and root morphology. The leaves, roots, and stems of rootstocks were dried to constant weight at 80°C, and each organ was weighed with a 1/1000 electronic balance. The root morphology was analyzed by WinRHIZO software (WinRHIZO version2012b, Regent Instruments Canada, Montreal, QC, Canada).
The dried samples were ground into powder and digested with H2SO4–H2O2. The N content was determined using the Kjeldahl apparatus (JK9870). The K content was determined using a flame photometer (M-410; Cole-Parmer, Chicago, IL, USA).
The dried samples were ground into powder and digested with H2SO4–H2O2. The N content was determined using the Kjeldahl apparatus (JK9870). The K content was determined using a flame photometer (M-410; Cole-Parmer, Chicago, IL, USA).
3
Soil Nutrient Analysis: N and K
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The N concentration in the digestion solution was determined with a continuous flow analyzer (AA3, Seal Analytical, Inc., Southampton, UK), and the K concentration in the digestion solution was determined with a flame photometer (M-410, Cole-Parmer, Chicago, IL, USA.).
4
Nutrient Analysis Protocol for Plant Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The harvested samples were digested with H 2 SO 4 -H 2 O 2 as described previously by Thomas [32] . Briefly, after the samples were dried to constant weight, about 0.1 g sample was taken into the digestive tube (50 mL). Then, 5 mL of 98% H 2 SO 4 was added into the tube and kept overnight. The digestion tube was put in the heating block and digested at 250 • C for 2 h with 5-10 drops of H 2 O 2 . The N and P concentrations in the digested solution were analyzed using a flow injection analysis instrument (FIAstar 5000 analyzer; FOSS, Hilleroed, Denmark). The K concentration in the digestion solution was measured using a flame photometer (M-410, Cole-Parmer, Chicago, IL, USA). Nutrient accumulation was calculated by multiplying the mineral concentration of each sample by the dry weight. Nutrient use efficiency was the ratio of dry weight and nutrient accumulation. Nutrient use index was defined as the ratio of dry weight and nutrient concentration.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!