Hs crp

ELISA kits (Cayman Chemical, MI, USA) for the determination of liver malondialdehyde (MDA) and superoxide dismutase (SOD) were done according to the written instructions. Serum levels of high sensitivity C-reactive protein (hs-CRP, ASSAYPRO, Saint Charles, MO, USA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) (Randox Laboratories, Antrim, UK) were measured as per the manufacturer’s instructions.

ELISA kits for the determination of blood and kidney tissue levels of highly sensitive C-reactive protein (hsCRP, ASSAYPRO, St. Charles, MO, USA), ALT (Randox Laboratories, Crumlin, UK), tumor necrosis factor-alpha (TNF-α, Abcam, Cambridge, UK), and lipid peroxidation measured as malondialdehyde (MDA, Cyaman Chemical, Ann Arbor, MI, USA) were used according to the manufacturer’s instructions. Blood urea and creatinine were measured using colorimetric methods (BioAssay System, Hayward, CA, USA). Serum levels of triglycerides (TGs), cholesterol, very-low-density lipoprotein (vLDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured using commercial kits supplied by SPINREACT, Coloma, Girona, Spain.