Irs 2 antibody

Growth media and supplements were purchased from Invitrogen (Grand Island, NY). IGF-I was purchased from GroPep (Adelaide, Australia). IGF-II was purchased from Gemini (Woodland, CA). Sulfasalazine (≥98%), L-Buthionine-sulfoximine (≥97%), N-Acetyl-L-cysteine, LY294002, U0126, and actin antibody were purchased from Sigma-Aldrich (St. Louis, MO). Humanized anti-IGF-IR monoclonal antibody huEM164 was generously provided by Immunogen Inc. (Norwood, MA). Antibodies for phosphorylated AKT serine 473, total and phospho-IGF-IR, total and phospho-phosphorylated p44/42 (MAPK), phospho-p38^MAPK, and IRS-1 were purchased from Cell Signaling Technology (Beverly, MA). The IRS-2 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The xCT antibody for Western blot analysis was purchased from Novus Biologicals (Littleton, CO). Horseradish peroxidase-conjugated anti-phosphotyrosine (PY-20) was purchased from BD Biosciences (San Jose, CA). Anti-rabbit and anti-mouse horseradish peroxidase-conjugated secondary antibodies were purchased from Pierce (Rockford, IL). 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate was purchased from Invitrogen (Carlsbad, CA).