After an overnight fast, blood samples were collected into ethylenediaminetetra-acetic acid (EDTA)-coated tubes. EDTA plasma and serum samples were aliquoted and stored at −80°C according to standard procedures until further analysis. Plasma Aβ40, Aβ42, t-tau and NfL were measured on a single molecule array (Simoa) platform (Quanterix Corp, MA, USA) following the manufacturer’s instructions (Wayen Biotechnologies Inc., Shanghai, China).30 (link) We used the Human Neurology 3-Plex A assay to measure plasma Aβ and t-tau and the NF-light® Advantage Kit for plasma NfL. We used ‘Vascular Injury Panel 2 (human)’ commercial kit from Meso Scale Discovery to quantify serum cytokine concentrations. The concentrations of serum-soluble intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were assayed following the manufacturer’s instructions. The quality control procedure was described elsewhere.31 (link)
Human neurology 3 plex a assay
Manufactured by Mesoscale
Sourced in China, United States
The Human Neurology 3-Plex A assay is a laboratory equipment product designed for the analysis of neurological biomarkers. It is a multiplex assay capable of simultaneously detecting and quantifying three specific analytes related to human neurology. The core function of this assay is to provide a comprehensive assessment of the targeted neurological parameters through a single experimental setup.
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Lab products found in correlation
2 protocols using human neurology 3 plex a assay
1
Plasma Biomarker Quantification for Neurological Assessment
2
Genetic and Biomarker Profiling for Alzheimer's
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Multiple‐PCR amplification analysis of genomic DNA was performed for 5508 participants to detect 67 single nucleotide polymorphism (SNP) risk loci (eg, APOE, translocase of outer mitochondrial membrane 40 [TOMM40], and KIdney and BRAin expressed protein [KIBRA] genes) and exon region of 20 genes that were potentially associated with AD and dementia.35 In a subsample from the same villages as the MRI subsample (n = 1452), plasma total‐tau, amyloid beta 40 (Aβ40), Aβ42, and neurofilament light chain (NfL) levels were measured on a single molecule array (SIMOA) platform (Quanterix Corp, MA, USA) with Human Neurology 3‐Plex A assay (N3PA) and NF‐light advantage Kit, following the manufacturers’ instructions.36 In addition, commercial kits (Meso Scale Discovery [MSD] V‐PLEX Assays) were used to measure serum cytokines and vascular injury biomarkers (n = 1872): interferon gamma (IFN‐γ), interleukin‐6 (IL‐6), interleukin‐8 (IL‐8), interleukin‐10 (IL‐10), tumor necrosis factor alpha (TNF‐α), interleukin‐17A (IL‐17A), eotaxin‐3, monocyte chemotactic protein‐1 (MCP‐1), intercellular cell adhesion molecule‐1 (ICAM‐1), and vascular cell adhesion molecule‐1 (VCAM‐1).
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