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CycB1 is a protein that plays a crucial role in cell cycle regulation. It functions as a regulatory subunit of the cyclin-dependent kinase 1 (CDK1) enzyme, which is essential for the progression of cells through the G2/M phase transition of the cell cycle.

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Lab products found in correlation

α sma, by Abcam (1 mentions) Gapdh hrp conjugate, by Cell Signaling Technology (1 mentions) Fastprep 24 5g bead beating grinder and lysis system, by MP Biomedicals (1 mentions) Ripa buffer, by Cell Signaling Technology (1 mentions) Protease inhibitor, by Roche (1 mentions) Cleavage caspase 3, by Cell Signaling Technology (1 mentions) P histone 3, by Cell Signaling Technology (1 mentions) Aurora a, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Mcl 1, by Cell Signaling Technology (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) P ampk, by Cell Signaling Technology (1 mentions) P p70s6k, by Cell Signaling Technology (1 mentions) Imagequant las 4000, by GE Healthcare (1 mentions) P 4ebp1, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions)

3 protocols using cycb1

1

Protein Analysis of Cultured Cells and Tissues

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Cultured cells were directly lysed in RIPA buffer (Cell Signaling Technology) supplemented with protease inhibitors (Roche Diagnostics) and phosphatase inhibitor cocktail (EMD Biosciences). For lung tissues, samples were resuspended in RIPA buffer and homogenized using the FastPrep-24 5G bead beating grinder and lysis system (MP Biomedicals). Samples were then centrifuged at 12,000 rpm for 15 minutes to obtain clear lysates for subsequent protein studies. Sources of antibodies were as follows: KLF4 (catalog ab129473) and α-SMA (catalog ab7817), Abcam; COL1α2 (catalog PA5-50938l), Thermo Fisher Scientific; Fas (catalog 05-201) and FOXM1 (catalog ABE1000), MilliporeSigma; and CYCB1 (catalog 4135) and GAPDH-HRP conjugate (catalog 3683), Cell Signaling Technology. All antibodies were used on both human and mouse samples at a dilution of 1:1000 except GAPDH-HRP, which was used at a dilution of 1:3000. Protein quantification was performed by densitometric analysis of blots using ImageJ software (NIH). Protein levels are presented as relative to the control value, set at 1. Full, uncut gels for all the Western blots are shown in the supplemental material.
Penke L.R., Speth J.M., Huang S.K., Fortier S.M., Baas J, & Peters-Golden M. (2022). KLF4 is a therapeutically tractable brake on fibroblast activation that promotes resolution of pulmonary fibrosis. JCI Insight, 7(16), e160688.
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2

Western Blot Analysis of Cell Signaling

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For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors. The antibodies used were as follows: HA (1:2000; Roche), CycG1 (1:500; Santa Cruz), PARP (1:1000; Cell Signaling), Cleavage-caspase-3 (1:500; Cell Signaling), Aurora A (1:1000; Cell Signaling), NOXA (1:1000; Millipore), Mcl-1 (1:1000; Cell Signaling), Bcl-2 (1:1000; Cell Signaling), Bcl-xL (1:1000; Cell Signaling), CycB1 (1:1000; Cell Signaling), p-Histone 3 (1:500; Cell Signaling) and β-actin (1:5000; Sigma).
Zhang W., Xu J., Ji D., Li Z., He W., Yang F., Lan H., Wang Y., Wu Z., Liu X., Huang S., Li L, & Zhou W. (2017). CyclinG1 Amplification Enhances Aurora Kinase Inhibitor-Induced Polyploid Resistance and Inhibition of Bcl-2 Pathway Reverses the Resistance. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 43(1).
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Western Blot Analysis of Cell Signaling

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Cell pellets were washed in ice-cold PBS and lysed using Ripa buffer freshly supplemented with protease inhibitor cocktail. Equal amounts of protein (15-30 ̀g) were loaded on a 12% SDS-PAGE and then transferred on a polyvinylidene diଏuoride membrane (Roche, Germany). After overnight incubation at 4 °C with speciଏc primary antibody and then the secondary antibody, proteins were visualized using ECL developing reagent on an Image Quant LAS 4000 (GE Healthcare, Germany). PARP (#9532), cleaved caspase 3 (#9661), CycE1 (#20 808), CycB1 (#12231), p53 (#2527), p73 (#14620), p27 (#3686), pAMPk (#2535), Myc (#13987), p-4EBP1 (#2855), and p-p70S6K (#9234) antibodies were purchased from Cell Signaling Technology. p21 (sc-6246) antibody was purchased from Santa Cruz Biotechnology. Tubulin (#T5168) was used as loading control and was obtained from Sigma-Aldrich.
Ripani P., Delp J., Bode K., Delgado M.E., Dietrich L., Betzler V.M., Yan N., von Scheven G., Mayer T.U., Leist M, & Brunner T. (2020). Thiazolides promote G1 cell cycle arrest in colorectal cancer cells by targeting the mitochondrial respiratory chain. Oncogene, 39(11).
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