EVs were separated and concentrated from 1 ml of plasma as described above and then analyzed by flow cytometry using CD9 exosome capture beads (ab239685, Abcam, Cambridge, UK) according to the manufacturer’s protocol. PE anti-human CD63 staining (10896786, BioLegend, Paris, France) was next performed. CD9-conjugated bead-coupled EVs were harvested by centrifugation and resuspended in 0.22 µm-filtered PBS. Analysis was performed in triplicate using BD FACS-CANTOII (CYTOCELL platform, SFR François Bonamy, Nantes, France) with 10,000 events recorded for each preparation. Histograms were mounted with FlowJo software version VX (BD, Ashland, OR, USA).
Pe anti human cd63 staining
Manufactured by BioLegend
Sourced in France
PE anti-human CD63 is a fluorescent-conjugated antibody used to detect the expression of CD63 on the surface of human cells. CD63 is a protein involved in the regulation of cell adhesion, motility, and signal transduction. This antibody can be used in flow cytometry applications to identify and quantify CD63-positive cell populations.
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Lab products found in correlation
2 protocols using pe anti human cd63 staining
1
Quantitative EV Characterization by Flow Cytometry
2
Flow Cytometric Analysis of Plasma Extracellular Vesicles
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EVs were separated and concentrated from 1 mL of plasma as described above and then analyzed by flow cytometry using CD9 exosome capture beads (ab239685, Abcam) according to the manufacturer's protocol. PE anti-human CD63 staining (10896786, BioLegend) was next performed. CD9-conjugated bead-coupled EVs were harvested by centrifugation and resuspended in 0.22 µm-filtered PBS. Analysis was performed in triplicate using BD FACS-CANTOII (CYTOCELL platform, SFR François Bonamy, France) with 10,000 events recorded for each preparation.
Histograms were mounted with FlowJo software.
Histograms were mounted with FlowJo software.
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