Islets were fixed overnight at 4°C in paraformaldehyde before application of primary antibodies against ADCY5/6 (cat. no. ab66037; Abcam) (34 (link)) and either guinea pig anti-insulin 1:200 or mouse anti-glucagon 1:1,000 (both DAKO). Revelation was performed with goat anti-rabbit Alexa-Fluor 488 and either goat anti–guinea pig Alexa-Fluor 568 or goat anti-mouse Alexa-Fluor 568 antibodies (both 1:500, Invitrogen). Images were acquired as previously described (27 (link)).
Mouse anti glucagon
Manufactured by Agilent Technologies
Sourced in Denmark
Mouse anti-glucagon is a laboratory reagent used for the detection and quantification of glucagon, a hormone produced by the pancreas that plays a key role in regulating blood glucose levels. This product provides a targeted and specific means of analyzing glucagon levels in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Guinea pig anti insulin, by Agilent Technologies (2 mentions)
Alexa fluor 568 goat anti guinea pig, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Hoescht, by Merck Group (1 mentions)
Rabbit anti somatostatin, by Agilent Technologies (1 mentions)
2 protocols using mouse anti glucagon
1
Immunofluorescent Staining of Pancreatic Islets
2
Immunohistochemical Analysis of Pancreatic Islets
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Pancreases were harvested, fixed 5h in 4% PFA and paraffin-embedded. Immunofluorescence staining was performed on 3 μm sections following standard procedures. Primary antibodies were: guinea pig anti-insulin (1:1000 dilution, Dako, Glostrup, Denmark), mouse anti-glucagon (1:500 dilution, Dako) and rabbit anti-somatostatin (1:500, Dako). Cye3 anti-guinea pig and Cy2 anti-rabbit or anti-mouse labeled secondary antibodies (1:500 dilution, Jackson Immunoresearch, Suffolk, UK) were used. Hoescht (1:500 dilution, Sigma-Aldrich) was employed as nuclear marker. Images were taken with a Leica DMR HC epifluorescence microscope and analyzed using Image J software. For morphometric measurements, 13 to 15 non-consecutive 3 μm thick sections (45 μm apart) were analyzed per pancreas.
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