Pcmv n egfp plasmid

PLL (molecular weight, 15–30 kDa) and 4',6‐diamidino‐2‐phenylindole dihydrochloride (DAPI) were purchased from Sigma (Taufkirchen, Germany), and PLL was dissolved in double‐distilled water at 5 mg/ml as a stock solution. High glucose Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), Opti‐MEM medium and Lipofectamine 2000 were obtained from Invitrogen Corp. (Carlsbad, CA, USA). 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide (MTT) was obtained from Amresco (Solon, OH, USA). Fluorescein isothiocyanate (FITC)‐labelled oligodeoxynucleotide (FITC‐ODN), 21 nucleotides with a random sequence, was purchased from RiboBio (Guangzhou, China). Calcein‐AM was purchased from Meilunbio (Dalian, China). The luciferase assay system (E1501) and pGL3‐Control plasmids (Photinus pyralis luciferase under the control of the SV40 promoter) were obtained from Promega (Madison, WI, USA). pCMV‐N‐EGFP plasmid was purchased from Beyotime Biotechnology (Shanghai, China). The BCA protein assay kit was obtained from Thermo Fisher Scientific Inc. (Rochester, NY, USA).