Proliferation of immortalized ASMC was assessed by colorimetric immunoassay based on the measurement of BrdU incorporation during DNA synthesis (Cell Proliferation Elisa; Abcam) according to the manufacturer's instructions. Briefly, cells starved for 12 hours in DMEM without FBS and were stimulated with 100 ng/mL MIF for 24 hours. After preincubation, ASMCs were labeled with BrdU for 2 hours. BrdU incorporation was detected by adding an anti-BrdU-POD antibody and subsequent substrate reaction.
Cell proliferation elisa
Manufactured by Abcam
Sourced in Germany
Cell Proliferation ELISA is a laboratory assay used to measure cell proliferation. The assay is based on the detection of a DNA synthesis marker incorporated into newly synthesized DNA of proliferating cells.
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Lab products found in correlation
2 protocols using cell proliferation elisa
1
Measurement of ASMC Proliferation
2
Breast Cancer Cell Proliferation Assay
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Cell proliferation was measured by BrdU incorporation using a BrdU Cell Proliferation ELISA according to the manufacturer's instructions (ab126556-BrdU Cell Proliferation kit, Abcam, Germany). Briefly, breast cancer cells (2 x 10 5 cells/mL) were treated with MET in a 96-well microplates for 24 h at 37 °C in a humidified atmosphere and 5% CO 2 . Thereafter, 20 µL of the diluted 1x BrdU was added to each well and cells were incubated overnight. Cells were then fixed and BrdU incorporation detected using anti-BrdU monoclonal Detector Antibody for 1 h at room temperature before incubation with peroxidase goat anti-mouse IgG conjugate as secondary antibody. Color was developed using tetramethylbenzidine (TMB) as a peroxidase substrate and BrdU incorporation measured at 450 nm using an ELISA reader (Biochrom Anthos 2020, Cambridge, UK).
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