GelMA (8% and 20% w/v) was added into glass bottom dishes (D110300, Matsunami, Osaka, Japan) and maintained at 4 °C for 10 minutes. Subsequently, distilled water was added to the gel. We use these procedures to keep surface structure stable and ensure reliable AFM measurement. GelMA was then crosslinked with 395 nm UV light for 2 minutes and maintained overnight at room temperature before further measurement. The elasticity of GelMA hydrogel were evaluated using atomic force microscopy (NanoWizard® 3 NanoOptics, JPK Instruments, Berlin, Germany). Briefly, a cantilever with 5 μm diameter spherical beads attached to the tip (CP-CONT-BSG-A, NanoAndMoreGmbH, Watsonville, CA) and Hertzian contact model were used to obtain force and displacement values, and Young’s modulus was calculated using JPK DP Data Processing Software (JPK Instruments). At least three specimens were used for each testing condition.
Jpk dp data processing software
Manufactured by Bruker
The JPK DP Data Processing Software is a product designed for data processing and analysis. It provides tools for handling and interpreting data generated by various analytical instruments. The software aims to facilitate efficient data management and extraction of meaningful insights from experimental results.
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Lab products found in correlation
2 protocols using jpk dp data processing software
1
Elastic Modulus of GelMA Hydrogels
2
Characterizing Polyacrylamide Gel Stiffness
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Gels containing sulfo-SANPAH were prepared to contain equivalent amounts of acryloyl groups in NHS-AA ester gels (Table 2). After polymerization of AA and bis-AA, 0.5 mL of 0.2 mg/mL sulfo-SANPAH (Thermo Fisher Scientific) was applied to the gel surface under 365 nm UV light at RT for 10 min. After the binding reaction with sulfo-SANPAH, the gel was washed three times with 2 mL PBS. These gels were proteincoated similar to NHS-AA esters gels. The Young's modulus of elasticity, which represents the stiffness of PA gels, was measured with AFM for each type of gel. The prepared PA gels were washed twice with 2 mL PBS and placed in a 35-mm dish. To measure their actual stiffness, the gels were immersed in PBS and force was measured on a NanoWizard three NanoOptics atomic force microscope (JPK Instruments, Berlin, Germany). Young's modulus was calculated for each force curve using JPK DP Data Processing Software (JPK Instruments), which uses a Hertzian contact model.
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