Cell transfection was performed with Lipofectamine 2000 or 3000 Reagent (Invitrogen, CA, USA) according to the manufacturer’s protocol. The two siRNA against circTP63 and the si-negative control (si-NC) oligos were purchased from Gene Pharma (Shanghai, China). The sequences for si-circTP63-1, si-circTP63-2 or si-NC were as follows: si-circTP63-1,5′-GCCAACAGUGAGGGGCCGU-3′; si-circTP63-2,5′-CAACAGUGAGGGGCCGUGAGA-3′; si-NC, 5′-UUCUCCGAACGUGUCACGU-3′. MiR-217 mimic, miR-217 inhibitor and miR-NC were obtained from Gene Pharma (Shanghai, China).
Mir 217 mimic
Manufactured by GenePharma
Sourced in China
MiR-217 mimic is a synthetic double-stranded RNA molecule designed to mimic the function of the naturally occurring microRNA (miRNA) miR-217. MiR-217 is involved in the regulation of gene expression. The MiR-217 mimic can be used in research applications to study the biological roles and mechanisms of miR-217.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (5 mentions)
Mir 217 inhibitor, by GenePharma (4 mentions)
Si circtp63, by GenePharma (3 mentions)
3000 reagent, by Thermo Fisher Scientific (3 mentions)
Mir nc, by GenePharma (3 mentions)
Si circtp63 1, by GenePharma (2 mentions)
Sirnacontrol si nc, by GenePharma (2 mentions)
Si nc, by GenePharma (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Liposome 3000, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Solarbio (1 mentions)
Streptomycin, by Solarbio (1 mentions)
Lentivirus, by GenePharma (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by GenePharma (1 mentions)
Sh mapk1, by GenePharma (1 mentions)
Sh crnde, by GenePharma (1 mentions)
Anti mir 217, by GenePharma (1 mentions)
Pcdna3.1 crnde, by GenePharma (1 mentions)
7 protocols using mir 217 mimic
1
Transfection of circTP63 and miR-217
2
Modulation of Inflammatory Cytokines in LO2 Cells
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The human liver LO2 cells were purchased in American Type Tissue Culture Collection. The cells were cultured in the medium of DMEM (Gibco, USA) containing 10% fetal bovine serum (Gibco, USA), 0.1 mg/mL streptomycin (Solarbio, China) and 100 units/mL penicillin (Solarbio, China) at a condition of 37 °C with 5% CO 2 . The lentivirus carrying the CASC7 shRNA, miR-217 mimic, CASC7 overexpression vector, TLR4 overexpression vector and corresponding control were obtained (GenePharma, China). The transfection in the cells was performed by Liposome 3000 (Invitrogen, USA) according to the manufacturer's instructions. The levels of TNF-α and IL-1β were measured by the ELISA assays in the cells.
3
Transfection of PDLSCs with EZH2, miR-217
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EZH2 overexpression vector, miR-217 mimic, miR-217 inhibitor, miR mimic control (NC-mimic), and miRNA inhibitor control (NC-inhibitor) all purchased from GenePharma (Shanghai, China) and were transfected into PDLSCs using Lipofectamine 3000 reagent (Invitrogen, USA), with all procedures following the manufacturer's protocol.
4
Transfection of circTP63 siRNA and miR-217
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Cell transfection was performed with Lipofectamine 2000 or 3000 Reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. The siRNA sequences for transfection were purchased from GenePharma (Shanghai, China).The sequences for circTP63 was as follows:si-circTP63-1, 5'-GCCAACAGUGAGGGGCCGU-3';si-circTP63-2,5'-CAACAGUGAGGGGCCGUGAGA-3';siRNAcontrol(si-NC)5'-UUCUCCGAACGUGUCACGU-3'.MiR-217mimic, miR-217 inhibitor and miR-NC were obtained from Gene Pharma (Shanghai, China).
5
Transfection of circTP63 siRNA and miR-217
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Cell transfection was performed with Lipofectamine 2000 or 3000 Reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. The siRNA sequences for transfection were purchased from GenePharma (Shanghai, China).The sequences for circTP63 was as follows:si-circTP63-1, 5'-GCCAACAGUGAGGGGCCGU-3';si-circTP63-2,5'-CAACAGUGAGGGGCCGUGAGA-3';siRNAcontrol(si-NC)5'-UUCUCCGAACGUGUCACGU-3'.MiR-217mimic, miR-217 inhibitor and miR-NC were obtained from Gene Pharma (Shanghai, China).
6
Characterizing CRNDE and MAPK1 in HepG2 and Huh-7 Cells
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PcDNA3.1‐CRNDE, sh‐CRNDE, pcDNA3.1‐MAPK1, sh‐MAPK1, miR‐217 mimics, anti‐miR‐217 and negative control were provided by GenePharma (Shanghai, China). Transfection of HepG2 and Huh‐7 cells was conducted using Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA). Transfected cells were cultured in 6‐well plates. After 48‐h cultivation, the cells were collected for subsequent analyses.
7
Transfection of HCC cells with miR-217 and HOTAIR modulators
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Si-HOTAIR, miR-217 mimics, the miR-217 inhibitor, and negative control were provided by GenePharma (Shanghai, China). A density of 2 × 105 Huh-7, Hep3B, SNU-387, and SNU-449 cells were plated into six-well plates and supplemented with 2 mL culture medium. The transfection of HCC cells was conducted using Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer's instructions. After 48 h cultivation, the cells were collected for subsequent experiments. The sequences were provided as followed:
Hsa-miR-217-5p mimics:
5'-UACUGCAUCAGGAACUGAUUGGA-3', 5'-CAAUCAGUUCCUGAUGCAGUAUU-3';
Hsa-miR-217-5p inhibitor:
5'-UCCAAUCAGUUCCUGAUGCAGUA-3';
HOTAIR-Homo-536:
Sense:5'-GCCUUCCUUAUAAGCUCGUTT-3'; Antisense:5'-ACGAGCUUAUAAGGAAGGCTT-3';
HOTAIR-Homo-1162:
Sense: 5'-CAAUAUAUCUGUUGGGCGUTT-3';
Antisense: 5'- ACGCCCAACAGAUAUAUUGTT-3';
HOTAIR-Homo-1597:
Sense: 5'- GGAAGCUCUUGAAGGUUCATT-3';
Antisense: 5'- UGAACCUUCAAGAGCUUCCTT -3';
Negative Control:
Sense:5'- UUCUCCGAACGUGUCACGUTT-3';
Antisense: 5'- ACGUGACACGUUCGGAGAATT-3';
Hsa-miR-217-5p mimics:
5'-UACUGCAUCAGGAACUGAUUGGA-3', 5'-CAAUCAGUUCCUGAUGCAGUAUU-3';
Hsa-miR-217-5p inhibitor:
5'-UCCAAUCAGUUCCUGAUGCAGUA-3';
HOTAIR-Homo-536:
Sense:5'-GCCUUCCUUAUAAGCUCGUTT-3'; Antisense:5'-ACGAGCUUAUAAGGAAGGCTT-3';
HOTAIR-Homo-1162:
Sense: 5'-CAAUAUAUCUGUUGGGCGUTT-3';
Antisense: 5'- ACGCCCAACAGAUAUAUUGTT-3';
HOTAIR-Homo-1597:
Sense: 5'- GGAAGCUCUUGAAGGUUCATT-3';
Antisense: 5'- UGAACCUUCAAGAGCUUCCTT -3';
Negative Control:
Sense:5'- UUCUCCGAACGUGUCACGUTT-3';
Antisense: 5'- ACGUGACACGUUCGGAGAATT-3';
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