Lentiviral particles were produced by cotransfecting 293T cells with pSIN-EF2-HLA-G1, pMD2.G, and psPAX2 plasmids using FuGENE 6 (Promega, Madison, WI). Vector-containing supernatants were collected 48 hours after transfection.The collected supernatants, for both retrovirus and lentivirus, were filtered with 0.2 μm low protein-binding syringe filters (Pall Corporation, Ann Arbor, MI), concentrated using 100K Amicon Ultra centrifugal filters (Millipore, Billerica, MA), and stored in aliquots at −80 °C until further use.
0.2 μm low protein binding syringe filters
Manufactured by Pall Corporation
The 0.2 μm low protein-binding syringe filters from Pall Corporation are designed to remove particulates and microorganisms from liquid samples. These filters have a pore size of 0.2 μm and are made with materials that minimize protein adsorption.
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Lab products found in correlation
2 protocols using 0.2 μm low protein binding syringe filters
1
Lentiviral Particle Production and Purification
2
Lentiviral Particle Production and Purification
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