1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylethanolamine

Lipids including 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE), 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol (Chol), and cardiolipin 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-sn-glycerol (TOCL) are purchased from Avanti Polar Lipids (Alabaster, AL). 5-doxyl stearic acid (5-SASL), TmCl₃·6H₂O, and VC (vitamin C) are purchased from Sigma-Aldrich (St. Louis, MO). C-terminal amidated peptide PrP106-126 (K¹⁰⁶TNMKHMAGAAAAGAVVGGLG¹²⁶-NH₂) is synthesized by the solid synthesis method. For simplicity, we will refer to the peptide as PrP106-126 without explicitly noting the amidation. Peptide purity is 98% as indicated by analytical HPLC elution. Fresh solutions containing monomeric PrP106-126 are prepared by dissolving lyophilized peptide in 10 mM HEPES pH 7.4. Centrifugation is used to remove preexisting insoluble aggregates.

1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE), soy l-α-phosphatidylinositol (PI), 1-oleoyl-2-(6-((4,4-difluoro-1,3-dimethyl-5-(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene-2-propionyl)amino)-hexanoyl)-sn-glycero-3-phosphoinositol (TopFluor TMR PI), 1-oleoyl-2-(6-((4,4-difluoro-1,3-dimethyl-5-(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene-2-propionyl)amino)-hexanoyl)-sn-glycero-3-phosphatidylethanolamine (TopFluor TMR PE), and 1-palmitoyl-2-{12-[(7-nitro-2–1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphatidylcholine (NBD PC) were purchased from Avanti Polar Lipids (Alabaster, AL). Buffer components tris(hydroxymethyl)-aminomethane (Tris), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium chloride, ethylenediaminetetraacetic acid (EDTA), dithiothreitol (DTT), and benzamidine were purchased from Fisher Scientific (Pittsburgh, PA). Dodecyl thiomaltopyranoside was purchased from Anatrace (Maumee, OH). The buffer solution used for all experiments contained 50 mM Tris, 600 mM HEPES, 300 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 4 mM benzamidine, and 24.7 μM Dodecyl thiomaltopyranoside (pH 8.0) (referred to as Amot ACCH domain elution buffer).