Cells were seeded at densities of ∼300,000 cells/cm2 onto the top side of transwell filter membranes (Corning Inc.) and left to migrate under normal culture conditions for 24 hrs. The number of migrated cells on the bottom of the membrane are proportional to the number of cells added on the top in a given set of experiments, which allows for comparisons across conditions by normalizing to a control sample.
Transwell filter membranes
Manufactured by Corning
Sourced in United States
Corning Transwell filter membranes are a laboratory product designed for cell culture and filtration applications. The membranes are made of a porous material that allows for the passage of fluids, cells, and other small molecules while retaining larger particles. The core function of Transwell filter membranes is to facilitate the study of cell migration, permeability, and transport processes in a controlled in vitro environment.
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Lab products found in correlation
2 protocols using transwell filter membranes
1
Cell Migration Assay on Transwell
2
Polarized Vero and 293T Cell Culture
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Vero C1008 (obtained from ATCC, CRL-1587) and 293T (obtained from ATCC, ACS-4500) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (ThermoFisher, Waltham, MA, USA), 100 U/mL penicillin, and 0.1 mg/mL streptomycin at 37 °C under 5% CO2 in a humidified incubator. For the analysis of the polarized cells, Vero C1008 cells were seeded on Transwell filter membranes (Falcon/Corning, Corning, NY, USA) with a 1.0 μm pore size. The culture medium was replaced, and the resistance value was monitored daily using a Millicell ERS-2 Electrical Resistance System (Millipore Corp, Burlington, MA, USA) until polarization was established. At day 5 post-seeding, the cells with a resistance of 60 (±10) Ω were prepared for the following study.
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