Bms215 2

Manufactured by Thermo Fisher Scientific

The BMS215/2 is a laboratory equipment manufactured by Thermo Fisher Scientific. It is designed to perform basic liquid handling tasks within a controlled laboratory environment.

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Lab products found in correlation

Bms224 2, by Thermo Fisher Scientific (2 mentions) Eh2il6, by Thermo Fisher Scientific (1 mentions) Bms223 4, by Thermo Fisher Scientific (1 mentions) Bms221 2, by Thermo Fisher Scientific (1 mentions) Bms228, by Thermo Fisher Scientific (1 mentions) Bms213 2, by Thermo Fisher Scientific (1 mentions)

5 protocols using bms215 2

Quantifying Inflammatory Cytokines by ELISA

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Enzyme-linked immunosorbent assays (ELISA) were utilized for the quantification of inflammatory antigens in the cell supernatant. The assessed markers included human Interleukin (IL)-1β, IL-4, IL-6, IL-10 and Tumor necrosis factor α (TNF-α). All ELISA kits were obtained from Thermo Fisher Scientific, with the following kit numbers: BMS224-2 (IL-1β), BMS225-2 (IL-4), EH2IL6 (IL-6), BMS215-2 (IL-10) and BMS223-4 (TNF-α). All reagents were maintained at room temperature before use. Cytokine markers were detected at a wavelength of 450 nm, as per manufacturer’s protocols. A standard curve was used for each assay to determine the cytokine concentration levels of the samples, as per manufacturer’s protocols and expressed in pg/mL.

Bachnak L., Sparks J., Newmire D.E., Gonzales X.F, & Omoruyi F.O. (2021). The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers. Biomedicines, 9(9), 1276.

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Cytokine Profiling of CAR T Cells

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CAR T cells or control T cells were cocultured with target cells (K562-PDL1-GL, HeLa-GL or H460-MSLN-GL cells) at a 1:1 E:T ratio for 24 h, and then the culture supernatants were collected. The production of IL-2 (ThermoFisher, BMS221-2), IFN-γ (ThermoFisher, BMS228), IL5 (ThermoFisher, BMS278), IL10 (ThermoFisher, BMS215-2) and IL13 (ThermoFisher, BMS231-3) was measured with enzyme-linked immunosorbent assay (ELISA) kits. All ELISAs were performed according to the manufacturer’s protocols.

Qin L., Cui Y., Yuan T., Chen D., Zhao R., Li S., Jiang Z., Wu Q., Long Y., Wang S., Tang Z., Pan H., Li X., Wei W., Yang J., Luo X., Zhang Z., Tang Q., Liu P., Weinkove R., Yao Y., Qin D., Thiery J.P, & Li P. (2022). Co-expression of a PD-L1-specific chimeric switch receptor augments the efficacy and persistence of CAR T cells via the CD70-CD27 axis. Nature Communications, 13, 6051.

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Synergistic CD19 T cell Activation

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Primary human CD4+ T cells were transduced with the α-CD19 synNotch Gal4VP64 receptor and 5× Gal4 response elements controlling human PD-L1 T2A IL-10 expression. The synNotch T cells were stimulated with either surface CD19+ or CD19- K562s for 24 hr and supernatant was harvested for IL-10 ELISA analysis (eBiosciences #BMS215/2). The T cells were also collected and stained separately for intracellular IL-10 with α-IL-10 APC (Biolegend #501410) and for surface PD-L1 with α-PD-L1 BV421 (Biolegend #329714).

Roybal K.T., Williams J.Z., Morsut L., Rupp L.J., Kolinko I., Choe J.H., Walker W.J., McNally K.A, & Lim W.A. (2016). Engineering T cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell, 167(2), 419-432.e16.

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Perioperative Inflammatory Biomarker Profiling

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Venous blood was withdrawn during hospital admission and 7 days postoperation. The plasma was obtained with a centrifugation for 15 min at 1780 ×g and −4°C; 300 μL aliquots have been stored at −80°C until use. The plasma levels of interleukin- (IL-) 1β, IL-6, IL-8, IL-10, IL-12, tumor necrosis factor- (TNF-) α, and C-reactive protein (CRP) were measured by enzyme-linked immunosorbent assay using the kits purchased from eBioscience (BMS224/2, BMS213/2, BMS204/3CE, BMS215/2, BMS238CE, BMS223/4CE, and 88-7502-28, resp.) according to the manufacturer's instructions. All samples were plated in duplicates, with average concentrations used for further analysis.

Ponasenko A.V., Kutikhin A.G., Khutornaya M.V., Rutkovskaya N.V., Kondyukova N.V., Odarenko Y.N., Kazachek Y.V., Tsepokina A.V., Barbarash L.S, & Yuzhalin A.E. (2017). Inherited Variation in Cytokine, Acute Phase Response, and Calcium Metabolism Genes Affects Susceptibility to Infective Endocarditis. Mediators of Inflammation, 2017, 7962546.

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Measuring Inflammatory Biomarkers in Plasma

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Plasma levels of monocyte chemotactic protein‐1 (MCP‐1), interleukin (IL)‐10, and IL‐12 were measured using the enzyme‐linked immunosorbent assay kits (BMS281, BMS215/2 and BMS238, respectively; eBioscience, San Diego, CA), and the ratio of IL‐10 to IL‐12¹² were calculated for the assessment of systematic inflammation.

Sun Q.H., Liu Z.J., Zhang L., Wei H., Song L.J., Zhu S.W., He M.B, & Duan L.P. (2021). Sex‐based differences in fecal short‐chain fatty acid and gut microbiota in irritable bowel syndrome patients. Journal of Digestive Diseases, 22(5), 246-255.

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