9 to 11 weeks old mice were used for MRI. MRI experiments were performed in a 7.0T, 16-cm horizontal Bruker MR imaging system (Bruker, Billerica, MA) with Bruker ParaVision 4.0 software. Mice were anesthetized with 1.5-3 % isoflurane and imaged with ECG, temperature and respiratory detection using a 38 mm Bruker birdcage volume coil. Magnevist (Bayer HealthCare, Montville, NJ) diluted 1:10 with sterile 0.9% saline, was administered intravenously at 0.3 mmol Gd /kg. T1 weighted gradient echo cine images of the heart were acquired in short axis from base to apex (6 slices) with the following parameters: repetition time TR = 10 to 11 ms, echo time TE= 3.4 ms, 10 to 13 frames, 30 degree flip angle, 3-4 averages, 1.0 mm slice thickness, 2.8 to 3.0 cm field of view, 256×256 matrix, respiratory and ECG-gated. Cardiac MRI data was processed to determine ejection fractions, ventricular volumes and associated functional parameters using CAAS-MRV-FARM software (Pie Medical Imaging, Netherlands.) Imaging experiments were conducted following institutional animal care and use guidelines.
Birdcage volume coil
Manufactured by Bruker
The Birdcage volume coil is a type of radio frequency (RF) coil used in magnetic resonance imaging (MRI) systems. It is designed to generate a uniform magnetic field within a specific region of interest, enabling the acquisition of high-quality MRI images. The core function of the Birdcage volume coil is to provide the necessary RF excitation and signal detection for MRI experiments.
Automatically generated - may contain errors
3 protocols using birdcage volume coil
1
Cardiac MRI Assessment in Mice
2
Cardiac MRI Assessment in Mice
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9 to 11 weeks old mice were used for MRI. MRI experiments were performed in a 7.0T, 16-cm horizontal Bruker MR imaging system (Bruker, Billerica, MA) with Bruker ParaVision 4.0 software. Mice were anesthetized with 1.5-3 % isoflurane and imaged with ECG, temperature and respiratory detection using a 38 mm Bruker birdcage volume coil. Magnevist (Bayer HealthCare, Montville, NJ) diluted 1:10 with sterile 0.9% saline, was administered intravenously at 0.3 mmol Gd /kg. T1 weighted gradient echo cine images of the heart were acquired in short axis from base to apex (6 slices) with the following parameters: repetition time TR = 10 to 11 ms, echo time TE= 3.4 ms, 10 to 13 frames, 30 degree flip angle, 3-4 averages, 1.0 mm slice thickness, 2.8 to 3.0 cm field of view, 256×256 matrix, respiratory and ECG-gated. Cardiac MRI data was processed to determine ejection fractions, ventricular volumes and associated functional parameters using CAAS-MRV-FARM software (Pie Medical Imaging, Netherlands.) Imaging experiments were conducted following institutional animal care and use guidelines.
3
In Vivo MRI Protocol for Rodent Imaging
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For in vivo MRI, anesthesia was induced and maintained in the same way as during the surgery. The animals were placed in an MRI-compatible rats cradle. The respiratory rhythm was monitored by a pressure sensor linked to a monitoring system (ECG Trigger Unit HR V2.0, RAPID Biomedical, Rimpar, Germany). MRI acquisitions were performed on a horizontal 7T BRUKER Biospec MRI system (Bruker Biospin MRI GbmH Bruker, Germany) equipped with a set of gradients of 440 mT/m and controlled via Bruker ParaVision 5.1 workstation. A Bruker birdcage volume coil (inner diameter = 72 mm and outer diameter = 112 mm) was used for the signal transmission, and a Bruker single loop surface coil (25 mm diameter) was used for signal reception. Two sequences were used: anatomical T2-weighted imaging (T2WI) and diffusion tensor imaging (DTI). Supplementary Table S1 presents the acquisition parameters.
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