The XTC-YF cell line derived from X. laevis tadpoles20 (link) (RIKEN BioResource Research Center, Ibaraki, Japan) was maintained in 0.5 × L-15 medium for cultured cells (0.5 × Leibovitz's L-15 medium (Merck, Darmstadt, Germany), 10% fetal bovine serum [FBS; BioWest, Nuaillé, France], 2 mM L-glutamine [Wako, Osaka, Japan], and 1% penicillin–streptomycin mixture [Nacalai Tesque, Kyoto, Japan]) at 25 °C.
Penicillin streptomycin mixture
Manufactured by Nacalai Tesque
Sourced in Japan
Penicillin–streptomycin mixture is a common antibiotic combination used in cell culture applications. It is a sterile solution containing the antibiotics penicillin and streptomycin.
Automatically generated - may contain errors
Lab products found in correlation
Rnaimax, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Foetal bovine serum (fbs), by Merck Group (1 mentions)
Caco 2 cell line, by American Type Culture Collection (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
D5796, by Merck Group (1 mentions)
Heat inactivated, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Orlistat, by Adooq (1 mentions)
Triacsin c, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Polyethylenimine max, by Polysciences (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
6 protocols using penicillin streptomycin mixture
1
Culturing X. laevis tadpole-derived XTC-YF cells
2
Culturing Human Colon Adenocarcinoma Caco-2 Cells
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Human colon adenocarcinoma Caco-2 cell line was obtained from American Type Culture Collection, ATCC (Manassas, VA). The cells were cultured in complete Minimum Essential Media (MEM) with Earle’s salt (Nacalai Tesque, Kyoto, Japan) supplemented with 10% foetal bovine serum (FBS) (Sigma, St. Louis, MO) and 100 units/ml penicillin-streptomycin mixture (Nacalai Tesque, Kyoto, Japan) for complete growth. The cell cultures were maintained in humidified atmosphere at 37 °C and 5% CO2.
3
Culturing PC12 Cells in Optimized Medium
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PC12 cells were maintained in Dulbecco’s Modified Eagle Medium (D5796, Sigma-Aldrich, St. Louis, MO, USA; 11885, Gibco, Grand Island, NY, USA) containing the following reagents: 10% fetal bovine serum (FBS), 10% horse serum (Gibco), and 1× penicillin/streptomycin mixture (Nacalai Tesque, Inc. Kyoto, Japan) at 37°C in 5% CO2 condition.
4
Mouse Lymphoma EL4 Cell Culture
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Mouse lymphoma EL4 cells (RCB1641) were obtained from the RIKEN BioResource Research Center (Tsukuba, Japan). EL4 cells were maintained in RPMI 1640 medium supplemented with heat-inactivated fetal calf serum (Sigma-Aldrich, St. Louis, MO, USA) and a penicillin–streptomycin mixture (Nacalai Tesque, Kyoto, Japan).
5
Rat Adipose-Derived Stem Cell Isolation and Characterization
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MSCs were obtained from AT of 6-8 weeks old male rats (180 ± 20 g) as previously described by De Luna-Saldivar et al. [4 (link)]. The isolated ASCs were cultured in Dulbecco’s modified eagle’s medium (DMEM, Sigma-Aldrich, St Louis, MO, USA) containing 10% fetal bovine serum (FBS, Biosolutions International, Melbourne, Australia) and 1% penicillin–streptomycin mixture (Nacalai Tesque, Kyoto, Japan) at 37 °C in a 5% CO2 humidified incubator. The medium was changed twice a week. Cells at 80%–85% confluence were trypsinized and passaged until the fourth passage. Then the cells were washed and suspended in PBS for use.
Characterization of ASCs for the expression of the cell surface markers, MSC markers (CD90, CD105 and CD73) and hematopoietic markers (CD34 and CD45), by FCM was performed [25 (link)]. Furthermore, characteristics of ASCs for their expression to CD73, CD105, CD90, CD34 and CD45 genes were confirmed by qRT-PCR as described later.
Characterization of ASCs for the expression of the cell surface markers, MSC markers (CD90, CD105 and CD73) and hematopoietic markers (CD34 and CD45), by FCM was performed [25 (link)]. Furthermore, characteristics of ASCs for their expression to CD73, CD105, CD90, CD34 and CD45 genes were confirmed by qRT-PCR as described later.
6
Cell Culture and Compound Treatment Protocols
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HeLa (Kyoto), HEK293T, and HepG2 cells were maintained at 37°C with 7% CO2 in DMEM (Gibco) supplemented with 10% fetal bovine serum (Sigma) and a penicillin/streptomycin mixture (final 100 U/ml and 100 µg/ml, respectively; Nacalai Tesque). Human visceral preadipocytes (Lonza) were maintained and differentiated into mature adipocytes with the PGM-2 Bullet Kit (Lonza) according to the manufacturer’s instructions. Plasmids and siRNAs were transfected into cells using Polyethylenimine MAX (Polysciences) and RNAiMAX (Thermo Fisher Scientific), respectively. OA (Merck) was conjugated to BSA (fatty acid and endotoxin free; Sigma) at a molar ratio of 6:1 in PBS before use. Cells were treated with the OA–BSA complex containing a final concentration of 200–400 µM OA or with a corresponding dose of BSA alone for 16–24 h. Triacsin C (Sigma) and orlistat (AdooQ BioScience) were diluted in DMSO and added to the medium at final concentrations of 5 µM for 6–24 h and of 100 µM for 24–48 h, respectively. Interferon-γ (PBL Assay Science) was added at the concentrations indicated in the figures for 24 h. BFA (Nacalai Tesque) was added at a final concentration of 5 µg/ml for 6–12 h.
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