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Wash buffer wa126

Manufactured by R&D Systems
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Sourced in United States

Wash Buffer WA126 is a ready-to-use aqueous solution designed for use in various laboratory techniques that require a washing step. The buffer is formulated to maintain the stability and integrity of samples during the washing process.

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Lab products found in correlation

Methylene chloride, by ITW Reagents (2 mentions) Reagent diluent dy995, by R&D Systems (2 mentions) Stop solution dy994, by R&D Systems (2 mentions) Substrate reagent pack dy999, by R&D Systems (2 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Resomer rg 502, by Evonik (1 mentions)

2 protocols using wash buffer wa126

1

Fabrication of PLGA-Dexamethasone Nanoparticles

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Poly (D, L-lactide-co-glycolide) (PLGA) 50:50 (12,000 g/mol) was purchased from Evonik Industries (Essen, Germany). Dexamethasone was supplied by Sigma-Aldrich (St. Louis Mo., USA) (purity > 98%). Fibronectin, fibronectin ELISA and reactants (Reagent Diluent DY995, Wash Buffer WA126, Stop Solution DY994 and Substrate Reagent Pack DY999) were purchased from R&D Systems (Minneapolis, MN, USA). Polyvinyl alcohol (PVA, 67,000 g/mol) was obtained from Merck KGaA (Darmstadt, Germany), and methylene chloride was obtained from PanReac AppliChem (Barcelona, Spain).
Rodrigo M.J., Bravo-Osuna I., Subias M., Montolío A., Cegoñino J., Martinez-Rincón T., Mendez-Martinez S., Aragón-Navas A., Garcia-Herranz D., Pablo L.E., Herrero-Vanrell R., del Palomar A.P, & Garcia-Martin E. (2022). Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma. Scientific Reports, 12, 20622.
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2

PLGA Nanoparticle Fabrication Protocol

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Poly (D, L-lactide-co-glycolide) (PLGA) 50:50 (inherent viscosity: 0.16–0.24 dL/g) (RESOMER® RG 502) was acquired from Evonik Industries (Essen, Germany). Methylene chloride was purchased from PanReac AppliChem (Barcelona, Spain), and polyvinyl alcohol 67 kDa (PVA) was acquired from Merck KGaA (Darmstadt, Germany). Fibronectin (CF 1918-FN), fibronectin ELISA and ELISA reactants (Reagent Diluent DY995, Wash Buffer WA126, Substrate Reagent Pack DY999, and Stop Solution DY994) were obtained from R&D Systems (Minneapolis, MN, USA).
Munuera I., Aragon-Navas A., Villacampa P., Gonzalez-Cela M.A., Subías M., Pablo L.E., Garcia-Feijoo J., Herrero-Vanrell R., Garcia-Martin E., Bravo-Osuna I, & Rodrigo M.J. (2023). Chronic Glaucoma Induced in Rats by a Single Injection of Fibronectin-Loaded PLGA Microspheres: IOP-Dependent and IOP-Independent Neurodegeneration. International Journal of Molecular Sciences, 25(1), 9.
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