B. burgdorferi strains N40, B31, and JD1 were used for this study. Spirochetes were grown in BSK II supplemented with 6% rabbit serum (Sigma-Aldrich, Hamburg, Germany) at 34 °C to a density of 108/mL [23 (link)]. Eight ml of this culture was then pelleted down by centrifuging at 7000× g for 10 min at RT.
Bsk 2
Manufactured by Merck Group
Sourced in Germany, United States
The BSK-II is a laboratory instrument designed for conducting bioseparation and chromatography experiments. It is a self-contained system that provides precise control and monitoring of parameters such as temperature, flow rate, and pressure during the separation process. The BSK-II is a versatile and reliable tool for researchers and scientists working in fields such as biochemistry, biotechnology, and pharmaceutical development.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using bsk 2
1
Culturing Borrelia burgdorferi
2
Culturing and Antibody Targeting of B. burgdorferi
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Different strains and mutants of B. burgdorferi (see Table S1 in the supplemental material) (41 (link), 64 (link)) were grown in BSK-II supplemented with 6% rabbit serum (Sigma) at 33°C and, for some experiments, at pH 6.7 at 35°C. Murine IgG1 monoclonal antibodies to OspA (65 (link)) and OspB (66 (link)), murine and rabbit polyclonal antibodies to OspC (67 (link)) and asialo GM1 (Abcam), and rabbit polyclonal antibody to HtrA (68 (link)) were used for different procedures. Mouse and rabbit antibodies to OspC were generated from a recombinant construct made from plasmid pET46 LIC, provided by Richard T. Marconi (Virginia Commonwealth University). The ∆ospA and ∆ospB mutants were provided by Thomas Schwan, and the ∆ospC and B313 strains were provided by Patricia Rosa (both from the Rocky Mountain Laboratories, NIH). Plasmid pBSV2G-ospC was also provided by Patricia Rosa (41 (link)), and plasmid pKFSS1 was provided by Scott Samuels from the University of Montana (69 (link)).
3
Cultivating Borrelia burgdorferi Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B. burgdorferi sensu stricto strains CA4, CA8, wild type 297 (WT297), BmtA mutant strains OY04/04 and mock-complemented OY07/F6218 (link) strains were cultured in Barbour– Stoner–Kelly II (BSK-II) complete medium, with 6% rabbit serum (Sigma, St Louis, MO, USA). The procedure for construction of BmtA mutants was explained in Ouyang et al.18 (link) The cultures were incubated at 33°C and maintained in sterile 50 mL falcon tubes. All culture media were sterilized with 0.2 μM filter units (Millipore, Billerica, MA, USA). The B. burgdorferi cultures were grown for 7–10 days to reach the stationary phase for drug screening.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!