Bcabest dig labeling kit

Manufactured by Takara Bio

Sourced in Germany

The BcaBEST DIG labeling kit is a laboratory product designed for labeling nucleic acid probes with digoxigenin (DIG). It provides a simple and efficient method for incorporating DIG-labeled nucleotides into DNA or RNA probes, enabling their detection in various molecular biology applications.

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Lab products found in correlation

Pcr dig probe synthesis kit, by Roche (1 mentions) Restriction endonuclease bglii, by Takara Bio (1 mentions) Cdp star detection reagent, by GE Healthcare (1 mentions) Las 1000 plus gel documentation system, by Fujifilm (1 mentions) Anti digoxygenin ap fab fragments, by Roche (1 mentions)

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BcaBEST Labeling Kit (9 citations)

2 protocols using bcabest dig labeling kit

Analyzing Mouse Mitochondrial DNA

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Purified total mtDNA was either left undigested or digested by incubation with restriction endonuclease BglII (Takara Bio, Shiga, Japan). After electrophoresis and depurination, alkaline blotting was performed by capillary transfer.
mtDNAs were detected using the Digoxigein (DIG)-labeled DNA probe for mouse mtDNA, which was described previously.31 (link) The DIG-labeled DNA probes for the control region (D-loop), ND1, and COX1 were synthesized using the PCR DIG Probe Synthesis Kit (Roche Diagnostics, Mannheim, Germany), and whole mtDNA probe was synthesized from Taq I-digested mouse whole mtDNA using random primers and the BcaBEST DIG labeling kit (Takara).

Fuke S., Kametani M., Yamada K., Kasahara T., Kubota-Sakashita M., Kujoth G.C., Prolla T.A., Hitoshi S, & Kato T. (2014). Heterozygous Polg mutation causes motor dysfunction due to mtDNA deletions. Annals of Clinical and Translational Neurology, 1(11), 909-920.

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Genomic DNA Analysis of C. orbiculare

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Genomic DNA of C. orbiculare was isolated from mycelia, and DNA blot analysis was done by standard methods. DNA probes were labeled with DIG-dUTP using the BcaBEST DIG Labeling Kit (Takara Bio). Hybridized DNA was detected using Anti-Digoxygenin-AP Fab fragments (Roche Diagnostics), and light emission generated by enzymatic dephosphorylation of CDP-Star Detection Reagent (GE Healthcare) by alkaline phosphatase was detected using the FUJIFILM LAS1000 plus gel documentation system (Fujifilm).

Kodama S., Ishizuka J., Miyashita I., Ishii T., Nishiuchi T., Miyoshi H, & Kubo Y. (2017). The morphogenesis-related NDR kinase pathway of Colletotrichum orbiculare is required for translating plant surface signals into infection-related morphogenesis and pathogenesis. PLoS Pathogens, 13(2), e1006189.

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