Analytical grade standards of biologically active ingredients of botanicals (curcumin, demetoxycurcumin, bisdemetoxycurcumin, α-boswellic acid, β-boswellic acid, 11-keto-β-boswellic acid, 3-acetyl-11-keto-β-boswellic acid, neoandrographolide and piperine) were purchased from Sigma-Aldrich (St. Louis, MO, USA), while andrographolide (≥98.0%) was obtained from TCI (Tokyo, Japan). Warfarin (PESTANAL®, analytical standard) was obtained by Sigma-Aldrich. Buffer solutions were prepared using phosphate buffer saline (PBS) tablets (Sigma Aldrich), di-sodium hydrogen phosphate dihidrate (buffer substance for chromatography) and sodium dihydrogen phosphate dihydrate (EMSURE® reagent Ph. Eur.) both by Merck KGaA, Darmstadt, Germany. Organic solvents n-octanol (gradient grade for liquid chromatography, ≥99%) and methanol (gradient grade for liquid chromatography LiChrosolv® Reag. Ph Eur.) as well as formic acid (for LC-MS, LiChropur®, 97.5–98.5%) and dimethyl sulfoxide (suitable for HPLC, ≥99.7%) were obtained by Merck KGaA. Dead volume of HPLC system was evaluated using sodium nitrate (reagent for USP/NF monographs) by J.T. Baker, Gliwice, Poland. Ultrapure water was produced using an Ultra Clear UV water purifying system (SG Water, Barsbuttel, Germany); resistivity > 18 MΩ/cm at 25 °C and total organic carbon < 5 ppb.
α boswellic acid
Manufactured by Merck Group
Sourced in United States
α-boswellic acid is a chemical compound that can be used in laboratory settings. It is a pentacyclic triterpene acid derived from the Boswellia serrata plant. The core function of α-boswellic acid is to serve as a research tool for scientific investigations, particularly in the fields of biochemistry and pharmacology.
Automatically generated - may contain errors
Lab products found in correlation
β boswellic acid, by Merck Group (2 mentions)
Warfarin, by Merck Group (1 mentions)
Piperine, by Merck Group (1 mentions)
N octanol, by Merck Group (1 mentions)
Lichropur, by Merck Group (1 mentions)
Neoandrographolide, by Merck Group (1 mentions)
Phosphate buffer saline tablet, by Merck Group (1 mentions)
3 o acetyl β boswellic acid, by Merck Group (1 mentions)
S aureus atcc 29213, by American Type Culture Collection (1 mentions)
S aureus atcc 25923, by American Type Culture Collection (1 mentions)
S aureus usa300, by American Type Culture Collection (1 mentions)
Maslinic acid, by Merck Group (1 mentions)
Mrsa 252, by American Type Culture Collection (1 mentions)
Arjunolic acid, by Merck Group (1 mentions)
Glycyrrhizic acid, by Merck Group (1 mentions)
S aureus usa400, by American Type Culture Collection (1 mentions)
3 protocols using α boswellic acid
1
Quantitative Analysis of Botanical Compounds
2
Analysis of Boswellic Acid Standards
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The reference standards keto boswellic acid (1 ), 3-O-Acetyl 11-keto β-Boswellic acid (2 ), α-Boswellic acid (3 ), β-Boswellic acid (4 ), 3-O-Acetyl-ɑ-boswellic acid (5 ) and 3-O-Acetyl-β-Boswellic acid (6 ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The purity of reference standards was >98% (Confirmed with HPLC and MS). Acetonitrile and formic acid were obtained from BioSolv (ULC/MS Grade) (Dieuze, France). Ammonium hydroxide solution (25%), methanol and Milli Q water were procured from Merck KGaALi Chrosolv LC-MS grade (Darmstadt, Germany).
3
Antibiotic Resistance and Oleanolic Acid Evaluation
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All bacterial strains
are listed inTables S1 and S2 . S. aureus USA300; S. aureus USA400, MRSA 252; S. aureus ATCC
29213; S. aureus ATCC 25904; and S. aureus ATCC 25923 were purchased from American
Type Culture Collection (ATCC). Animal- and human-origin clinical
isolates, including MRSA, E. coli,
and K. pneumoniae, were collected in
Shandong and Jilin, China; these isolates carried one or more β-lactamases.27 (link)S. aureus 8325-4
was obtained from Prof. Timothy J. Foster.15 (link)E. coli BL21(DE3)(pET28a-SP-NDM-1)
carried an NDM-1 gene originating from K. pneumoniae QD-KP2. E. coli BL21(DE3)(pET21a)
was used as a type C β-lactamase-positive strain. In addition, E. coli BL21(DE3)(pET28a) and S. aureus ATCC 25923 were used as negative control strains.
OA and its
analogues (corosolic acid (CA), ursolic acid, maslinic acid, glycyrrhizic
acid, α-boswellic acid, and arjunolic acid) (Figure S1 ) were purchased from Sigma-Aldrich, St. Louis, MO.
All antibiotics were purchased from the National Institute for the
Control of Pharmaceutical and Biological Products (Beijing, China).
are listed in
29213; S. aureus ATCC 25904; and S. aureus ATCC 25923 were purchased from American
Type Culture Collection (ATCC). Animal- and human-origin clinical
isolates, including MRSA, E. coli,
and K. pneumoniae, were collected in
Shandong and Jilin, China; these isolates carried one or more β-lactamases.27 (link)S. aureus 8325-4
was obtained from Prof. Timothy J. Foster.15 (link)E. coli BL21(DE3)(pET28a-SP-NDM-1)
carried an NDM-1 gene originating from K. pneumoniae QD-KP2. E. coli BL21(DE3)(pET21a)
was used as a type C β-lactamase-positive strain. In addition, E. coli BL21(DE3)(pET28a) and S. aureus ATCC 25923 were used as negative control strains.
OA and its
analogues (corosolic acid (CA), ursolic acid, maslinic acid, glycyrrhizic
acid, α-boswellic acid, and arjunolic acid) (
All antibiotics were purchased from the National Institute for the
Control of Pharmaceutical and Biological Products (Beijing, China).
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