Gadph sc 47724

Human Caco-2 cells (HTB-37), human normal colon CCD-33Co cells (CRL-1539), Eagle’s Minimum Essential Medium, and 0.25% (w/v) trypsin-0.53 mM EDTA were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). Penicillin-streptomycin was purchased from Corning Inc. (Corning, NY, USA), fetal bovine serum (FBS) from Hyclone (Thermo Scientific Hyclone, Logan, UT, USA). VitaBlue 20% anthocyanin blueberry extract (N208.2) was provided by FutureCeuticals, Inc. (Momence, IL, USA). Primary mouse monoclonal antibody GADPH (sc-47724), primary rabbit polyclonal antibody GAPDH (sc-25778), primary goat polyclonal antibody HNF-1α (N-19) (sc-6548), donkey polyclonal anti-goat IgG horseradish conjugate secondary antibody (sc-2020), purified donkey polyclonal anti-goat IgG horseradish conjugate secondary antibody (sc-2056), and purified goat polyclonal anti-rabbit IgG horseradish conjugate secondary antibody (sc-2054)were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Sheep polyclonal anti-mouse IgG horseradish conjugate secondary antibody (NXA931) was purchased from GE Healthcare Biosciences (Pittsburg, PA). All other chemicals were purchased from Sigma-Aldrich unless otherwise stated.

PTx was from Sigma (St. Louis, MO, USA). Trastuzumab was from Genetech (South San Francisco, CA, USA). Laptinib, GDC0941 and saracatinib were from LC Laboratories (Woburn, MA, USA). Antibodies for AKT (no. 4685), phospho-AKT^S473 (no. 4060), Src (no. 2109), and phospho-Src^Y416 (no. 6943) were from Cell Signaling Technology (Danver, MA, USA); GADPH (sc-47724) was from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies for mouse FITC-conjugated CD24 (#101806), allophycocyanin-conjugated CD49f (#313616), and phycoerythrin-conjugated CD61 (#104308) were from Biolegend (San Diego, CA, USA). GCV was from TCI America (Portland, OR, USA).