Alexa fluor 488 conjugated streptavidin

Manufactured by Jackson ImmunoResearch

Sourced in Panama, United States

Alexa Fluor 488-conjugated streptavidin is a fluorescent conjugate that binds to biotin with high affinity. It is used in various immunoassay and cell biology applications as a detection reagent.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor plus 555, by Thermo Fisher Scientific (2 mentions) Leitz dmrb microscope, by Leica camera (2 mentions) Vt1000s vibratome, by Leica Microsystems (2 mentions) Vectashield, by Vector Laboratories (2 mentions) Triton x 100, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Lsm 880 confocal microscope, by Zeiss (2 mentions) Tsa plus dig kit, by PerkinElmer (1 mentions) Alexa 594 conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Ab75838, by Abcam (1 mentions) Ab137029, by Abcam (1 mentions) Alexa fluor plus 800, by Thermo Fisher Scientific (1 mentions) Alexa fluor plus 488, by Thermo Fisher Scientific (1 mentions) Biotinylated anti mouse igm, by Jackson ImmunoResearch (1 mentions) Mounting medium, by Merck Group (1 mentions) Anti c fos sc 52, by Santa Cruz Biotechnology (1 mentions) Cm3050s cryostat, by Leica camera (1 mentions) A1 confocal microscope, by Nikon (1 mentions) Mab318, by Merck Group (1 mentions)

17 protocols using alexa fluor 488 conjugated streptavidin

Dual FISH Assay for Prss56 and Tshb in Rat Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dual FISH was performed on sections from three adult rats, by mixing the digoxigenin-labeled Prss56 and fluorescein-labeled Tshb probes. Following hybridization, sections were first incubated in the peroxidase-conjugated sheep anti-digoxigenin antibody, and the signal was amplified using the TSA Plus DIG Kit (Cat# NEL748E001KT, Perkin Elmer) for 30 min, applying the DIG amplification reagent at 1:500 dilution in 0.05M Tris (pH 7.6) containing 0.01% H₂O₂. Sections were then incubated in a rabbit monoclonal antibody against digoxigenin (Thermo Fisher, Cat# 700772, RRID:AB_2532342; at 1 μg/ml concentration) for 3h, in the presence of 2% sodium azide to inactivate peroxidase activity. Sections were thoroughly washed in PBS, and incubated overnight in peroxidase-conjugated sheep anti-fluorescein antibody (Roche, Cat# 11426346910, RRID:AB_840257; diluted 1:100 in 1% blocking reagent). Signal amplification was applied using the TSA Plus Biotin Kit as described above, and the signals were detected with the cocktail of Alexa Fluor 488-conjugated Streptavidin and Alexa 594-conjugated anti-rabbit IgG (Jackson; 1:200). The red and green fluorescence of Alexa 594 and Alexa 488, respectively, were swapped in the images to keep the green color consistent for Prss56.

Wittmann G, & Lechan R.M. (2018). Prss56 expression in the rodent hypothalamus: Inverse correlation with proopiomelanocortin suggests oscillatory gene expression in adult rat tanycytes. The Journal of comparative neurology, 526(15), 2444-2461.

+ Open protocol

+ Expand

Immunofluorescence Profiling of Neuronal Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used in this study: mouse anti-EEA1 (1:25, BD Biosciences Cat# 610456), mouse anti-GABA_B2 (1:400, Abcam ab181736), rabbit anti-GABA_B2 (1:400, Abcam ab75838), rabbit anti-GABA_B2N (1:250, custom-made by GeneScript, Benke et al., 2002 (link)), mouse anti-HA-tag (1:400, Sigma Aldrich, H9658), rabbit anti-NeuN (1:400, Millipore ABN78), rabbit anti-PP2A (1:400, MyBioSource MBS858915), mouse anti-Rab4 (1:25, BD Biosciences Cat# 610888) and rabbit anti-Rab7 (1:50, Abcam ab137029). Secondary antibodies: donkey anti-rabbit AlexaFluor Plus 488 (1:2000, Thermo Fisher Scientific A32790), donkey anti-mouse AlexaFluor Plus 555 (1:2000, Thermo Fisher Scientific A32773), goat anti-rabbit AlexaFluor Plus 800 (1:2000, Thermo Fisher Scientific A32735), and AlexaFluor 488-conjugated streptavidin (1:500, Jackson ImmunoResearch 016-540-084). All normal laboratory chemicals used were purchased from Sigma-Aldrich.

Hleihil M., Balakrishnan K, & Benke D. (2022). Protein phosphatase 2A regulation of GABAB receptors normalizes ischemia-induced aberrant receptor trafficking and provides neuroprotection. Frontiers in Molecular Neuroscience, 15, 1015906.

+ Open protocol

+ Expand

Visualizing Neuronal Morphology in Spinal Slices

Check if the same lab product or an alternative is used in the 5 most similar protocols

After physiologic assays, spinal slices were fixed in 4% paraformaldehyde overnight and then transferred to a 30% sucrose solution in PBS. Slices were stained against biocytin with 1:500 Alexa Fluor 488-conjugated streptavidin (Jackson ImmunoResearch) to visualize the morphology of the recorded cells. Slices were also stained for DAPI (Sigma-Aldrich) to identify laminar boundaries.

Gong N., Hagopian G., Holmes T.C., Luo Z.D, & Xu X. (2019). Functional Reorganization of Local Circuit Connectivity in Superficial Spinal Dorsal Horn with Neuropathic Pain States. eNeuro, 6(5), ENEURO.0272-19.2019.

+ Open protocol

+ Expand

Direct and Indirect Coombs Tests

Check if the same lab product or an alternative is used in the 5 most similar protocols

For direct Coombs tests, cells were collected from blood or spleen and probed directly with biotinylated anti-mouse IgM (Jackson ImmunoResearch) and the indicated phenotype antibodies for 30 min at 4°C. For indirect Coombs tests, cells were first incubated at 4°C for 2 hrs with the indicated mouse serum diluted 1:30 in PBS before being probed with biotinylated anti-mouse IgM and the indicated phenotype antibodies. Cells were then washed and probed with AlexaFluor-488 conjugated streptavidin (Jackson ImmunoResearch) followed by analysis by flow cytometry.

Ryan S.O., Abbott D.W, & Cobb B.A. (2014). Myeloid Glycosylation Defects Lead to Spontaneous a CVID-Like Condition with Associated Hemolytic Anemia and Anti-Lymphocyte Autoimmunity. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5561-5570.

+ Open protocol

+ Expand

Immunofluorescence Imaging of Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were anesthetized and perfused as described previously. The brain was isolated, fixed in 4% PBS-buffered PFA, and dehydration in sucrose solutions (30%) at 4 °C. The 50-μm frozen coronal slices were prepared by Leica CM3050 S Cryostat (Buffalo Grove, IL, USA). Slices were incubated in primary antibody in block buffer (10% goat serum and 0.2% Triton X-100) over night. After washing by PBS, the slices were incubated in secondary antibody for 1 h and DAPI for 5 min at room temperature. The slices were mounted in mounting medium (Sigma). Primary antibodies used were anti-HA (H6908-0.5 ml, Sigma, 1:500), anti-c-Fos (sc-52, Santa Cruz, Dallas, TX, USA), anti-CRH (ab8901, Abcam, Cambridge, MA, USA), anti-TH antibodies (MAB318, Merck, 1:1000), and DAPI (D9534, Sigma). Secondary antibodies were anti-mouse 488 (711-545-150, Jackson ImmunoResearch, 1:1000) and anti-rabbit Cy3 (115-165-116, Jackson ImmunoResearch, 1:500). Images were acquired on a Nikon-A1 confocal microscope (Tokyo, Japan) using a 20 × objective lens. Biocytin-backfill slices were incubated with Cy3- or Alexa Fluor-488-conjugated streptavidin (Jackson ImmunoResearch, 016-160-084, 016-540-084, 1:1000) to secondary antibody solution.

Jiang C., Yang X., He G., Wang F., Wang Z., Xu W., Mao Y., Ma L, & Wang F. (2021). CRHCeA→VTA inputs inhibit the positive ensembles to induce negative effect of opiate withdrawal. Molecular Psychiatry, 26(11), 6170-6186.

+ Open protocol

+ Expand

Histological Analysis of Injection Site

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the indicated times after immunization, mice were euthanized by CO₂ asphyxiation and the injection site samples were excised. The tissues were fixed in 4% paraformaldehyde for 3 h, dehydrated in 30% sucrose solution overnight, embedded in optimal cutting temperature medium, snap frozen in liquid nitrogen and stored at −80 °C. Eight micrometer thick sections of the tissue were obtained with a Leica CM1860 cryostat(Leica Biosystem, Buffalo Grove, IL). Sections were stained with the following antibodies (all from Biolegend, San Diego, CA): Biotinylated anti-I-A/I-E (#107603; APCs), anti-Ly-6G (#127603; neutrophils), anti-Mac-2 (#125403; macrophages), anti-Ly-6C (#128003; monocytes). The antibodies were detected with Alexa Fluor 488-conjugated streptavidin (Jackson Immunoresearch Laboratory, West Grove, PA). Eosinophils were stained with phenol red.^{34 (link)} Sections were then embedded in Prolong Gold antifading with 4',6-diamidino-2-phenylindole (Invitrogen, Carlsbad, CA), coverslipped, sealed, and stored at 4 °C. The number of fluorescently labeled cells at the injection site was counted within high-power field on a fluorescence microscope (Nikon E400). Confocal images of the injection sites were obtained with a Nikon A1R MP microscope (Nikon).

Lu F., Mosley Y.Y., Rodriguez Rosales R.J., Carmichael B.E., Elesela S., Yao Y, & HogenEsch H. (2017). Alpha-D-glucan nanoparticulate adjuvant induces a transient inflammatory response at the injection site and targets antigen to migratory dendritic cells. NPJ Vaccines, 2, 4.

+ Open protocol

+ Expand

Quantification and Visualization of Hyaluronan in OPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

A commercial HA enzyme‐linked immunosorbent assay (ELISA) kit (DuoSet ELISA System1: Hyaluronan, R&D Systems, Minneapolis, MN) was used to quantify HA levels in cell culture supernatants. To visualize levels of HA produced by OPCs, cells were cultured in OPCDM and incubated with a biotinylated HABP (bHABP; Seikagaku Kogyo Co., Tokyo, Japan); the HABP complex was detected with Alexa Fluor 488‐conjugated Streptavidin (Jackson ImmunoResearch).

Marella M., Ouyang J., Zombeck J., Zhao C., Huang L., Connor R.J., Phan K.B., Jorge M.C., Printz M.A., Paladini R.D., Gelb A.B., Huang Z., Frost G.I., Sugarman B.J., Steinman L., Wei G., Shepard H.M., Maneval D.C, & Lapinskas P.J. (2017). PH20 is not expressed in murine CNS and oligodendrocyte precursor cells. Annals of Clinical and Translational Neurology, 4(3), 191-211.

+ Open protocol

+ Expand

Brain Tissue Fixation and Sectioning

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice or rats were overdosed with pentobarbital injection (100 mg/kg body weight), perfused with saline and 4% paraformaldehyde before their brains were rapidly removed. After overnight post-fixation in 4% paraformaldehyde solution, the brain was washed 3 times in 0.1 M phosphate buffered saline. Coronal sections of 50–100 μm thickness were cut using a Leica Microsystems VT 1000S vibratome, wash 3–4 times in 0.1 M phosphate buffer (PB) and stored in 0.1 M PB with 0.05% sodium azide at 4°C. Sections were mounted on glass slides in VectaShield (Vector Laboratories) and observed with epifluorescence (Leitz DMRB microscope, Leica) or confocal imaging (Zeiss). For the identification of native EYFP + or streptavidin + neurons and layer markers we proceeded as previously described (35, 45). Sections containing Neurobiotin-labeled cells were localized by incubating them in 1:400 Alexa Fluor488–conjugated streptavidin (Jackson ImmunoResearch 016–540-084) with 0.5% Triton X-100 (vol/vol) in PBS (PBS-Tx) for 2 h at 22–25°C.

Valero M., Navas-Olive A., de la Prida L.M, & Buzsáki G. (2022). Inhibitory conductance controls place field dynamics in the hippocampus. Cell reports, 40(8), 111232.

+ Open protocol

+ Expand

Visualizing Neuronal Morphology with Biocytin Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

After electrophysiological recording, brain slices were fixed in 4% paraformaldehyde overnight, then transferred to 30% sucrose solution in PBS. Slices were stained against biocytin with 1:500 Alexa Fluor 488-conjugated streptavidin (Jackson ImmunoResearch) to visualize the morphology of recorded cells. Slices were also stained for 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) to identify laminar boundaries. Cell morphology was visualized using Olympus BX 61 epifluorescent microscopy and the MetaMorph imaging suite (Molecular Devices). In addition, we imaged labeled cells in selected sections with a confocal microscope (LSM 700/780, Carl Zeiss Microscopy). Image stitching, overlaying, maximum projections, and export were performed by using the ZEN software analysis tools.

Shi Y., Grieco S.F., Holmes T.C, & Xu X. (2019). Development of Local Circuit Connections to Hilar Mossy Cells in the Mouse Dentate Gyrus. eNeuro, 6(2), ENEURO.0370-18.2019.

+ Open protocol

+ Expand

SARS-CoV-2 Spike Protein Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Vero cells (5 × 10⁴) were seeded onto cover glasses on 12-well plates. After 24 h, the cells were replenished with DMEM/F12 medium and incubated with R-Spike CD-MERS-CoV-Biotin in a 5% CO₂ incubator for 30 min at 37 °C. The cells were fixed with 4% paraformaldehyde and subsequently blocked and permeabilized with phosphate-buffered saline with Tween-20 (PBST) containing 1% bovine serum albumin (BSA). Alexa Fluor-488-conjugated Streptavidin (Jackson ImmunoResearch laboratories Inc.) was added, and the cultures were incubated for 1 h. Then, the samples were washed in PBST, and Hoechst 33258 (Thermo Fisher Scientific) was added to stain the nuclei. The slides were analyzed by confocal microscopy using a Carl Zeiss LSM710 microscope (Carl Zeiss Co. Ltd. Oberkochen, Germany).

Park B.K., Kim J., Park S., Kim D., Kim M., Baek K., Bae J.Y., Park M.S., Kim W.K., Lee Y, & Kwon H.J. (2021). MERS-CoV and SARS-CoV-2 replication can be inhibited by targeting the interaction between the viral spike protein and the nucleocapsid protein. Theranostics, 11(8), 3853-3867.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!