Antiphosphotyrosine mouse mAb (pTyr-1000) beads were purchased from Cell Signaling Technology (Danvers, MA). TPCK-treated trypsin was obtained from Worthington Biochemical (Lakewood, NJ). DMEM/F12 with and without lysine and arginine, fetal bovine serum (FBS), l -glutamine, and antibiotics were purchased from Invitrogen (Carlsbad, CA). SILAC amino acids, 13C6-lysine, 13C6-arginine, 2H4-lysine, 13C6-arginine, 13C615N2-lysine, 13C615N4-arginine were purchased from Cambridge Isotope Laboratories (Andover, MA). All other reagents used in this study were from Fisher Scientific (Pittsburgh, PA).
2h4 lysine
Manufactured by Cambridge Isotopes
2H4-lysine is a stable isotope-labeled amino acid used in various analytical and research applications. It contains four deuterium atoms, which can be used as tracers in metabolic studies and other experiments. The product is available in different purities and quantities to accommodate the needs of researchers and laboratories.
Automatically generated - may contain errors
Lab products found in correlation
13c6 arginine, by Cambridge Isotopes (2 mentions)
Silac amino acids, by Cambridge Isotopes (1 mentions)
Dmem f12 with and without lysine and arginine, by Thermo Fisher Scientific (1 mentions)
13c615n2 lysine, by Cambridge Isotopes (1 mentions)
13c615n4 arginine, by Cambridge Isotopes (1 mentions)
13c6 lysine, by Cambridge Isotopes (1 mentions)
Tpck treated trypsin, by Worthington (1 mentions)
L lysine and l arginine, by Merck Group (1 mentions)
Dialyzed fbs, by Thermo Fisher Scientific (1 mentions)
2 protocols using 2h4 lysine
1
SILAC-Based Quantitative Proteomic Analysis
2
SILAC Labeling of HeLa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa cells were split from normal growth media into arginine and lysine-free DMEM (Caisson Laboratories Inc.) supplemented with 10% heat inactivated dialyzed FBS (Gibco) and either 1 mM 2H4-lysine and 0.1 mM 13C6-arginine (Cambridge Isotope Laboratories) for heavy cells or normal isotopic abundance of l -lysine and l -arginine (Sigma) for light cells. Cells were maintained in labelling media for at least 5 cell divisions to ensure complete labelling as described elsewhere28 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!