Goat anti flag

Western blot was performed as previously described (Fadó et al., 2015 (link)). Rabbit anti-CPT1C was developed in our laboratory (1:1,000; Sierra et al., 2008 (link)). Rabbit anti-ACC (1:1,000; 3676) and rabbit anti-phosphorylated ACC (Ser79; 1:2,000; 3661) were from Cell Signaling. Rabbit anti-SAC1 (1:500; 13033–1-AP) was from Proteintech. Mouse anti–β-actin (1:1,000; ab6276) and chicken anti-GFP (1:5,000; ab13970) were from Abcam. Rabbit anti-GluA1 (1:1,000; ab1504) and rabbit anti-GluA2 (1:1,000; ab397) were from Millipore. Rabbit anti-GluN2A (1:1,000; G9038), mouse anti–β-tubulin (1:2,000; T5201), and goat anti-FLAG (1:500; F3165) were from Sigma-Aldrich. HRP-conjugated secondary antibodies anti-mouse or anti-rabbit (1:10,000) were from DAKO. Blots were developed using Luminata Forte Western HRP substrate (Millipore). Semiquantitative analysis was performed using densitometry with Fiji software.

Antibodies and their manufacturers were: mouse anti-FLAG clone M2 (WB 1 : 4000, IF 1 : 400; F1804) and rabbit anti-LC3B (WB 1 : 1000, IF 1 : 200; L7543) were from Sigma–Aldrich; goat anti-FLAG (1 : 400; ab1257), rabbit anti-SQSTM1/p62 (IP 1–2 μg/mg of lysate; ab101266) and rabbit anti-GFP (WB 1 : 2000, ab290) were from Abcam; mouse anti-SQSTM1/p62 (WB 1 : 1000 IF 1 : 200; 610832) was from BD Biosciences; rabbit anti-SQSTM1/p62 (WB 1 : 1000, 8025S) was from Cell Signaling Technology; mouse anti-HA.11 clone 16B12 (WB 1 : 1000, 901501) was from Biolegend; mouse anti-HA clone 12CA5 (WB 1 : 2000; 11583816001) from Roche; mouse IgG isotype control (14-4732-82) was from Invitrogen; Alexa Fluor 488-, 555-, 594- and 647-conjugated secondary antibodies were from Thermo Fisher (A21206, A31570, A11032 and A21447, respectively).