Dca 200 vantage analyzer

Mice were fasted for 4 h, and blood samples were collected 35 days after transplantation. HbA1c levels from whole blood were measured using a DCA 200 Vantage Analyzer (Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and DCA Systems HbA1c Reagent Kit (Siemens Healthcare Diagnostics).

All blood parameters were measured using samples collected from 4 h fasted mice. Hemoglobin A1c (HbA1c) levels were measured using a Siemens DCA 200 Vantage Analyzer (Siemens Healthcare Diagnostics, Tarrytown, NY, USA) from whole blood. Plasma insulin (Mouse Ultrasensitive Insulin ELISA, ALPCO, Salem, NH, USA), glucagon (Glucagon ELISA, Mercodia, Salem, NC, USA), leptin (Mouse Leptin ELISA, Crystal Chem, Downers Grove, IL, USA), β-hydroxybutyrate (β-Hydroxybutyrate LiquiColor Test, Stanbio, Boerne, TX, USA), free fatty acids (HR Series NEFA HR[2] Kit, Wako Diagnostics, Richmond, VA, USA), triglycerides and glycerol (Serum Triglyceride Determination Kit, Sigma–Aldrich, Oakville, Canada), and cholesterol (Cholesterol E, Wako Diagnostics, Richmond, VA, USA) were measured from cardiac puncture blood samples. For samples from PEG-leptin injected mice, plasma leptin levels were analyzed using a PEG-leptin standard curve as the PEG-leptin was less potent in the assay than an equivalent amount of normal leptin.