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Pe cy5 conjugated anti mouse cd8

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The PE-Cy5 conjugated anti-mouse CD8 is a fluorescently labeled antibody that binds to the CD8 surface antigen on mouse T cells. It is commonly used in flow cytometry applications for the identification and analysis of mouse CD8+ T cell populations.

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Lab products found in correlation

Rat anti mouse cd16 32 antibody, by BD (3 mentions) Pe cy5 conjugated anti mouse cd3e, by BD (3 mentions) Pe cy5 conjugated anti mouse b220, by Thermo Fisher Scientific (3 mentions) Pe cy5 conjugated anti mouse gr 1, by Thermo Fisher Scientific (3 mentions) Pe conjugated anti mouse sca1, by Thermo Fisher Scientific (3 mentions) Apc conjugated anti mouse ckit, by Thermo Fisher Scientific (3 mentions) Pe cy5 conjugated anti mouse cd4, by Thermo Fisher Scientific (3 mentions) Facscanto, by BD (3 mentions) Fitc conjugated anti mouse cd48, by Thermo Fisher Scientific (2 mentions) Brilliant violet 421 conjugated anti mouse cd150, by BioLegend (2 mentions) 7 aad, by BD (2 mentions) Ack lysing buffer, by Lonza (2 mentions) Coulter counter, by Beckman Coulter (2 mentions) Pe cy5 conjugated anti mouse cd11b, by Thermo Fisher Scientific (1 mentions) Fitc conjugated anti mouse cd45, by Thermo Fisher Scientific (1 mentions)

3 protocols using pe cy5 conjugated anti mouse cd8

1

Multiparametric Flow Cytometry Analysis of Murine Hematopoietic Cells

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Whole bone marrow (WBM) cells were isolated from femurs and tibias by grinding the bones in HSC buffer. RBCs were lysed using ACK lysing buffer (Lonza). Total number of WBM cells was counted with a Coulter counter (Beckman Coulter). Three million WBM cells were blocked with a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with PE-Cy5 conjugated anti-mouse CD3e (clone: 145-2C11), PE-Cy5 conjugated anti-mouse CD4 (clone: GK1.5), PE-Cy5 conjugated anti-mouse CD8 (clone: 53-6.7) PE-Cy5 conjugated anti-mouse B220 (clone: RA3-6B2), PE-Cy5 conjugated PE-Cy5 conjugated anti-mouse CD11b (clone: M1/70), PE-Cy5 conjugated anti-mouse Gr-1 (clone: RB6-8C5), PE-Cy5 conjugated anti-mouse Ter119 (Ly-76), PE conjugated anti-mouse Sca1 (clone:D7), APC conjugated anti-mouse cKit (clone: 2B8), FITC conjugated anti-mouse CD48 (clone: HM48-1) (eBioscience) and Brilliant Violet 421 conjugated anti-mouse CD150 (clone: TC15-12F12.2) antibodies (BioLegend). All antibodies were diluted 1:400. Dead cells were excluded by staining with 7-AAD (BD Pharmingen). Data were collected from 1 million single cells by FACSCanto (BD Pharmingen) and analyzed by FlowJo (Tree Star, Inc) without knowledge of the genotype or treatment by a single observer (CLL).
Lee C.L., Castle K.D., Moding E.J., Blum J.M., Williams N., Luo L., Ma Y., Borst L.B., Kim Y, & Kirsch D.G. (2015). Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma. Nature Communications, 6, 8477.
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2

Murine Hematopoietic Stem Cell Immunophenotyping

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WBM cells were isolated from femurs and tibias by grinding the bones in HSC buffer. RBCs were lysed using ACK lysing buffer (Lonza). Total number of WBM cells was counted with a Coulter counter (Beckman Coulter). Three million WBM cells were blocked with a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with PE-Cy5 conjugated anti-mouse CD3e (clone: 145-2C11), PE-Cy5 conjugated anti-mouse CD4 (clone: GK1.5), PE-Cy5 conjugated anti-mouse CD8 (clone: 53-6.7) PE-Cy5 conjugated anti-mouse B220 (clone: RA3-6B2), PE-Cy5 conjugated anti-mouse CD11b (clone: M1/70), PE-Cy5 conjugated anti-mouse Gr-1 (clone: RB6-8C5), PE-Cy5 conjugated anti-mouse Ter119 (Ly-76), PE-conjugated anti-mouse Sca1 (clone:D7), APC conjugated anti-mouse cKit (clone: 2B8), FITC conjugated anti-mouse CD48 (clone: HM48-1; eBioscience) and Brilliant Violet 421 conjugated anti-mouse CD150 (clone: TC15-12F12.2; BioLegend) antibodies. All antibodies were diluted 1:400. Dead cells were excluded by staining with 7AAD (BD Pharmingen). Data were collected from 1 million single cells by FACSCanto (BD Pharmingen) and analysed by FlowJo (Tree Star, Inc.) without knowledge of the genotype or treatment by a single observer (C.L.L.).
Lee C.L., Castle K.D., Moding E.J., Blum J.M., Williams N., Luo L., Ma Y., Borst L.B., Kim Y, & Kirsch D.G. (2015). Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma. Nature Communications, 6, 8477.
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3

Comprehensive BM Cell Immunophenotyping

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Whole BM cells were isolated from femurs and tibias according to methods described elsewhere (16 (link)). A total of 3 × 106 live BM cells were blocked using a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with PE-Cy5 conjugated anti-mouse CD3e (clone: 145–2C11), PE-Cy5 conjugated anti-mouse CD4 (clone: GK1.5), PE-Cy5 conjugated anti-mouse CD8 (clone: 53–6.7) PE-Cy5 conjugated anti-mouse B220 (clone: RA3–6B2), PE-Cy5 conjugated PE-Cy5 conjugated anti-mouse CD11b (clone: M1/70), PE-Cy5 conjugated anti-mouse Gr-1 (clone: RB6–8C5), PE-Cy5 conjugated anti-mouse Ter119 (Ly-76), PE conjugated anti-mouse Sca1 (clone: D7), APC conjugated anti-mouse cKit (clone: 2B8), FITC conjugated anti-mouse CD45.2 (clone: 104; eBioscience) and APC-eFluor780 conjugated anti-mouse CD45.1 (clone: A20; e eBioscience). All antibodies were diluted 1:400. Data were collected from at least 500,000 single cells using FACSCanto (BD Pharmingen) and analyzed using FlowJo (Tree Star Inc.) without knowledge of the genotype or treatment by a single observer (C-LL).
Hasapis S., Caraballo I, & Lee C.L. (2021). Transplantation of Unirradiated Bone Marrow Cells after Total-Body Irradiation Prevents the Development of Thymic Lymphoma in Mice through Niche Competition. Radiation research, 195(3), 301-306.
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