Human liver tissues were stained with purified anti-TCR Vα7.2 (50μg/ml, 3C10, BioLegend) or IgG1 isotype control to detect the localisation of Vα7.2+ cells. See supplementary data .
Anti tcr vα7
Manufactured by BioLegend
Sourced in United States
Anti-TCR Vα7.2 is a lab equipment product that is used to detect the expression of the TCR Vα7.2 chain on T cells. It provides a tool for researchers to investigate the role of this specific T cell receptor subunit in various biological processes.
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5 protocols using anti tcr vα7
1
Visualizing Vα7.2+ Cells in Liver Tissue
2
Immunophenotyping of T-cell Subsets
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Monoclonal antibodies (mAb) used for immunofluorescence assay were anti-TCR-γδ-PE (Beckman Coulter, Brea, CA, USA), anti-CD161-FITC, anti-CD3-PerCP, anti-CD8-PerCP, anti-CD69-PE, anti-NKG2D-PE, anti-NKG2A-PerCP, anti-IL-7R-PE, anti-CD195-PE, anti-CD196-PerCP (Becton Dickinson), anti-TCR-Vα7.2-APC (BioLegend, San Diego, CA). Monoclonal antibody used for immune histochemical staining was anti-TCR-Vα7.2 (BioLegend).
3
Immunohistochemical Analysis of Tissue Markers
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Fresh bone marrow, liver tissue, and intestinal tissue were deparaffinized and hydrated after fixation with formaldehyde. Then, deparaffinization and antigen repair were performed on the tissue sections. Afterward, the sections were blocked with 10% goat serum for 30 min and incubated with anti-CD105 (Abcam, ab231774) and anti-TCR Vα7.2 (BioLegend, 351702) antibodies at 4°C overnight. Then, the sections were stained with fluorophore-labeled secondary antibodies (Cell Signaling Technology, 4409S and 4412S), and 4′,6-diamidino-2-phenylindole was used to stain cell nuclei. The sections were observed with a fluorescence microscopy according to the manufacturer's instructions.
4
Profiling MAIT Cell Responses in PBMCs
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Peripheral blood mononuclear cells (PBMCs) were obtained by Ficoll-Paque (GE Healthcare, USA) gradient centrifugation. Cell surface and intracellular staining was performed using the following monoclonal antibodies (mAbs): anti-TCR-Vα7.2 (Biolegend, USA), anti-CD3, anti-CD161, anti-CD69, anti-PD-1, anti-TNFα, anti-IFN-γ, anti-IL-4, anti-IL-10, anti-CCR5, anti-CXCR6 (BD Biosciences, USA). For intracellular staining, the Cytofix/CytopermTM Fixation/Permeabilization kit was utilized according to the manufacturer's specification (BD Biosciences, USA). Data were acquired by flow cytometry (BD LSRFortessa, USA), and analyzed with FlowJo software Version7.6 (Tree Star, Ashland, OR, USA).
5
Immunohistochemical Detection of Vα7.2+ Cells
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Human liver tissues were stained with purified anti-TCR Vα7.2 (50 μg/ml, 3C10, BioLegend) or IgG1 isotype control to detect the localisation of Vα7.2+ cells. See Supplementary material .
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