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Pannoramic scan 250 flash

Manufactured by 3DHISTECH
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The Pannoramic Scan 250 Flash is a high-speed digital slide scanner capable of scanning histological samples at a resolution of up to 0.125 microns per pixel. It features a focal plane array sensor and a high-intensity LED light source for efficient image capture.

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Lab products found in correlation

Pannoramic viewer 1, by 3DHISTECH (6 mentions) Cleaved caspase 3 asp175, by Cell Signaling Technology (2 mentions) Cd3 a0452, by Agilent Technologies (2 mentions) Axioimager z2 system, by Zeiss (2 mentions) Ki67 vp k451, by Vector Laboratories (2 mentions) Nbp2 37412, by Novus Biologicals (1 mentions) Secondary anti rabbit or anti mouse igg, by ZSGB-BIO (1 mentions) Secondary anti rabbit igg, by ZSGB-BIO (1 mentions) 3 3 diaminobenzidine (dab), by ZSGB-BIO (1 mentions)

7 protocols using pannoramic scan 250 flash

1

Histological Analysis of Organ Samples

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Organ samples were fixed in fresh 4% paraformaldehyde at 4 °C overnight and further subjected to routine histological procedures for embedding in paraffin. 5-μm sections of the samples from at least three different animals per group were placed on microscopic slides next to one another to enable cross-comparison within a slide after H&E staining or IHC staining with the antibodies indicated. Antibodies used were GFP (D5.1) XP, PTEN (D4.3), phospho (p)-AKT (S473) (D9E), p-ribosomal protein S6 (S235/236) (D57.2.2E), Cleaved Caspase-3 (Asp175) (all: Cell Signaling Technologies, Danvers, MA), Ki67 (VP-K451, Vector Laboratories, Burlingame, CA), and CD3 (A0452) (DAKO, Glostrup, Denmark). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images acquired using Pannoramic Viewer 1.15.2 (both: 3DHistech, Budapest, Hungary). Additional images were taken on a Zeiss Axio Imager.Z2 system.
Miething C., Scuoppo C., Bosbach B., Appelmann I., Nakitandwe J., Ma J., Wu G., Lintault L., Auer M., Premsrirut P.K., Teruya-Feldstein J., Hicks J., Benveniste H., Speicher M.R., Downing J.R, & Lowe S.W. (2014). PTEN action in leukemia dictated by the tissue microenvironment. Nature, 510(7505), 402-406.
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2

Histological Analysis of Organ Samples

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Organ samples were fixed in fresh 4% paraformaldehyde at 4 °C overnight and further subjected to routine histological procedures for embedding in paraffin. 5-μm sections of the samples from at least three different animals per group were placed on microscopic slides next to one another to enable cross-comparison within a slide after H&E staining or IHC staining with the antibodies indicated. Antibodies used were GFP (D5.1) XP, PTEN (D4.3), phospho (p)-AKT (S473) (D9E), p-ribosomal protein S6 (S235/236) (D57.2.2E), Cleaved Caspase-3 (Asp175) (all: Cell Signaling Technologies, Danvers, MA), Ki67 (VP-K451, Vector Laboratories, Burlingame, CA), and CD3 (A0452) (DAKO, Glostrup, Denmark). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images acquired using Pannoramic Viewer 1.15.2 (both: 3DHistech, Budapest, Hungary). Additional images were taken on a Zeiss Axio Imager.Z2 system.
Miething C., Scuoppo C., Bosbach B., Appelmann I., Nakitandwe J., Ma J., Wu G., Lintault L., Auer M., Premsrirut P.K., Teruya-Feldstein J., Hicks J., Benveniste H., Speicher M.R., Downing J.R, & Lowe S.W. (2014). PTEN action in leukemia dictated by the tissue microenvironment. Nature, 510(7505), 402-406.
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3

Immunohistochemical Analysis of CD3 Expression in Murine Spleen

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Immunohistochemistry was performed on the spleen of NOD/SCID mice to investigate changes in CD3 expression. The spleen tissues were incubated in 4% paraformaldehyde for 24 h. Then, the tissues were embedded into paraffin and sliced into 5-μm sections, followed by staining with CD3 antibody (1:500). The sections were placed at 4 °C overnight. The next day, the stained tissues were added with the secondary antibody (1:5000) and incubated for 2 h. After washing with PBS, the samples were scanned using a Pannoramic Scan 250 Flash (3DHISTECH Kft, Budapest, Hungary), and the images were captured using QCapture (QImaging, Burnaby, Canada) with QImaging on a Macintosh computer.
Xia T., Zhang J., Zhou C., Li Y., Duan W., Zhang B., Wang M, & Fang J. (2019). 20(S)-Ginsenoside Rh2 displays efficacy against T-cell acute lymphoblastic leukemia through the PI3K/Akt/mTOR signal pathway. Journal of Ginseng Research, 44(5), 725-737.
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4

Immunohistochemical Analysis of Liver Tissue

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Liver tissue samples were fixed in 4% paraformaldehyde and embedded in paraffin according to standard procedures. Sections were incubated with the indicated primary antibodies overnight at 4 °C. Thereafter, the slides were incubated with a secondary anti-rabbit or anti-mouse IgG antibody (ZSGB-BIO, Beijing, China) and visualized using 3,3'-diaminobenzidine (ZSGB-BIO). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system, and images were acquired using Pannoramic Viewer 1.15.2 (3DHistech, Budapest, Hungary).
Hu J., Gao Q., Yang Y., Xia J., Zhang W., Chen Y., Zhou Z., Chang L., Hu Y., Zhou H., Liang L., Li X., Long Q., Wang K., Huang A, & Tang N. (2021). Hexosamine biosynthetic pathway promotes the antiviral activity of SAMHD1 by enhancing O-GlcNAc transferase-mediated protein O-GlcNAcylation. Theranostics, 11(2), 805-823.
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5

Histopathological Analysis of Liver Fibrosis

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Liver specimens were fixed in 4% paraformaldehyde, embedded in paraffin and cut into 4 µm sections. Then, the specimens were deparaffinized, hydrated and stained by standard methods. To examine hepatic morphology and assess liver fibrosis, H&E and Sirius Red staining were performed. Sections were immune‐stained for SLC27A5 (1:500, NBP2‐37412, Novus Biologicals, CO, USA), α‐SMA (1:300, 19245T, CST, MA, USA), F4/80 (1:300, 70076T, CST), or RUNX2 (1:300, 20700‐1‐AP, Proteintech, IL, USA) overnight at 4 °C. Sections were then incubated with a secondary anti‐rabbit or anti‐mouse IgG (ZSGB‐BIO, Beijing, China) and stained using 3,3′‐diaminobenzidine (ZSGB‐BIO). Stained slides were scanned with a Pannoramic Scan 250 Flash and images were acquired using Pannoramic Viewer 1.15.2 (3DHistech, Budapest, Hungary). Immunostaining and Sirius Red staining were quantified by threshold analysis using the NIH ImageJ software. The immunohistochemical staining of SLC27A5 and RUNX2 was semi‐quantitatively analyzed using the immunoreactive scoring system.[62 (link)
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Wu K., Liu Y., Xia J., Liu J., Wang K., Liang H., Xu F., Liu D., Nie D., Tang X., Huang A., Chen C, & Tang N. (2023). Loss of SLC27A5 Activates Hepatic Stellate Cells and Promotes Liver Fibrosis via Unconjugated Cholic Acid. Advanced Science, 11(2), 2304408.
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6

Immunohistochemical Analysis of Tumor Samples

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Tumor tissue samples were fixed in 4% paraformaldehyde and embedded in paraffin according to standard procedures. Sections were incubated with the indicated primary antibodies overnight at 4 °C. Subsequently, the slides were incubated with a secondary anti-rabbit or anti-mouse IgG (ZSGB-BIO, Beijing, China) and visualized using 3,3′-diaminobenzidine (ZSGB-BIO). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images were acquired using Pannoramic Viewer 1.15.2 (3DHistech, Budapest, Hungary). Images were analyzed using Image Pro Plus software.
Li D., Yao Y., Rao Y., Huang X., Wei L., You Z., Zheng G., Hou X., Su Y., Varghese Z., Moorhead J.F., Chen Y, & Ruan X.Z. (2022). Cholesterol sensor SCAP contributes to sorafenib resistance by regulating autophagy in hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 41, 116.
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7

Immunohistochemical Analysis of Liver Samples

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Liver samples were fixed in fresh 4% paraformaldehyde and subjected to routine histological procedures for embedding in paraffin. Then, the samples were cut in to 4.5-μm sections, which were processed for hematoxylin and eosin (HE) staining or IHC staining with antibodies targeting PCK1(1:500), and β-catenin (1:500). For IHC assay, the sections were incubated with secondary anti-rabbit IgG (ZSGB-BIO, Beijing, China) and stained with 3,3′-diaminobenzidine (ZSGB-BIO). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images were acquired using Pannoramic Viewer 1.15.2 (3DHistech, Budapest, Hungary).
Xiang J., Zhang Y., Tuo L., Liu R., Gou D., Liang L., Chen C., Xia J., Tang N, & Wang K. (2019). Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq. Genes & Diseases, 7(1), 150-159.
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