MH-S cells were inoculated at a density of 5 × 103 cells/well in 96-well plates (701001, NEST Biotechnology Co. Ltd., Wuxi, China) for 24 h. The cells were treated with different concentrations of GDC-0068 (1, 5, 10, 20, 40, 60, 80, 150, and 200 μM) for 12 h. Then, the cells were washed with PBS and co-incubated with CCK-8 solution (#40203ES60, YEASEN, Shanghai, China) in a 37 °C incubator for 1 h. The Microplate Reader (Hangzhou Allsheng Instrument Co., Ltd., Hangzhou, China, FlexA-200) was used to detect the optical density (OD) values at 450 nm.
Cck 8 solution
Manufactured by Yeasen
Sourced in China
The CCK-8 solution is a colorimetric assay kit used for the determination of cell viability and cytotoxicity. It contains the tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to form a water-soluble formazan dye. The amount of formazan dye produced is directly proportional to the number of viable cells, allowing for the quantification of cell proliferation and cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Bio-Rad (6 mentions)
Microplate reader, by Agilent Technologies (4 mentions)
96 well plate, by Corning (4 mentions)
Microplate reader, by Thermo Fisher Scientific (4 mentions)
Microplate spectrophotometer, by Agilent Technologies (2 mentions)
Microplate reader, by Molecular Devices (2 mentions)
Cisplatin, by Merck Group (1 mentions)
Spark 10m, by Tecan (1 mentions)
Transwell plate, by Corning (1 mentions)
Microplate reader at 450 nm, by Molecular Devices (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Microplate reader, by Tecan (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
One step mycoplasma detection kit, by Yeasen (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Cisplatin, by MedChemExpress (1 mentions)
5 fluorouracil, by MedChemExpress (1 mentions)
Varioskan lux, by Thermo Fisher Scientific (1 mentions)
Synergy h4, by Agilent Technologies (1 mentions)
65 protocols using cck 8 solution
1
Evaluating GDC-0068 Cytotoxicity in MH-S Cells
2
Cytotoxicity Evaluation of miRNA
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A total of 5000 A2780 cells were pre-seeded in each well of the 96-well plates. the cells were transfected with miRNA when cells con uence upto 80%. after 0 h, 24 h, 48 h, and 72 h transfection,10µl of CCK8 solution(Yeasen, China) was added to each well and incubated for 1 hour and read the absorbance at 450nm by a microplate reader.
3
β-HB pretreatment modulates cisplatin-induced cytotoxicity
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The HK-2 cells (3 × 103/well) in 96-well plates were pretreated with different concentrations (10 μM, 20 μM, 30 μM) of β-HB (52017, Sigma, USA) for 24 h. Subsequently, 20 μM cisplatin (1134357, Sigma, USA) was added, while the control group was exposed to PBS. Following a 3 h, 6 h, 12 h, or 24 h incubation, CCK-8 solution (40203ES60, Yeasen, China) was introduced to the cell medium and incubated for an hour at 37 °C. The microplate reader determined the absorbance values at 450 nm (Thermo Scientific, USA). Cell viability = OD450/0h OD450.
4
CCK-8 Assay for Cell Viability
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For the CCK-8 assay, the transfected cells (1 × 103 cells/well) were seeded into 96-well plates in quintuplicate and cultured until they were entirely adherent. CCK-8 solution (10 μl, Yeasen, Shanghai, China) was added to each well at 0, 24, 48, 72, and 96 hours. After 2 hours of incubation, the absorbance at 450 nm was measured on a microplate reader (SpectraMax iD3).
5
Cell Viability Assessment via CCK-8 Assay
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The CCK-8 assay was used to analyze the cell viability. Cardiac fibroblasts were grown at 2–4 × 104 cells/well in 96-well plates and incubated in an incubator (37°C, 5% CO2). The CCK-8 solution (0.5 mg/mL, Yeasen Biotechnology) was subsequently added to the medium to a final concentration and incubated for 4 h at 37°C. Optical density values (570 nm) were then measured.
6
Cell Proliferation Assay Using CCK-8
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Tumor cells (1500/well) were seeded in 96-well plates in triplicate. Ten microliters of CCK-8 solution (Yeasen, Shanghai, China) was added to each well and incubated at 37 °C for 2 h. Then, the absorbance of the dye solution at 450 nm was measured. The optical densities of the cells were assessed 0, 24, 48, 72, and 96 h after incubation.
7
Cell Proliferation Assay using CCK-8
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After puromycin selection, the cells were seeded into 96-well plates at a density of 5000 cells per well (100 μL medium) in triplicate for each group and cultured for 0 h, 24 h, 48 h, and 72 h. At the indicated time points, 10 μL of the CCK-8 solution (Yeason, Shanghai, China) was added to each well and incubation was continued for 2 h at 37 °C, 5% CO2. The optical density (OD) was measured through a microplate reader (Biotek Instruments, Inc., Winooski, WT, USA) at 450 nm.
8
CCK-8 Assay for HeLa Cell Viability
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HeLa cells were inoculated into 96-well plates (2×103 cells/well) and were cultured at 37°C with 5% CO2 for 24, 48 or 72 h. Following the addition of 10 µl CCK-8 solution (Shanghai Yeasen Biotechnology Co., Ltd.), cells were cultured at 37°C for 3 h. A microplate reader (Bio-Rad Laboratories, Inc.) was then used to assess the absorbance in each well at 450 nm.
9
Colony Formation and Cell Viability Assays
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A total of 5 × 103 cells were seeded into 12-well plate (colony formation survival assay) or 96-well plates (CCK-8 assay) in triplicates overnight, treated with IR or the indicated drugs. For colony formation assays, cells were fixed after 10 days of treatment by methanol, stained with 0.2% crystal violet solution and photographed. Colonies consisting of >50 cells were counted. For CCK-8 assays, after 48 h of treatment, 10 μl CCK-8 solution (Yeasen, #40203ES60) was added to each well. The plates were incubated in an incubator for 3 h, and then absorbance at 450 nm was determined.
10
Assessing Cell Viability in 293T Cells
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Human embryonic kidney cells 293T were seeded directly into 96-well culture plates at 5×103 cells/well and cultured in 100 μl complete DMEM medium with 5 μM SB203580 and 1 μM BIRB0796 for 4, 24, 48, and 72 h. At the exact time, 10 μl CCK-8 solution (#40203ES76, Yeasen Biotechnology (Shanghai) Co., Ltd., China) was added to each well and incubated at 37°C for 1.5 h. By using an ELX-800 spectrometer reader, the absorbance was measured at 450 nm.
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