Rabbit specific hrp aec detection ihc kit

Cryostat liver tissue sections (4 μm) were fixed and permeabilised in ice cold acetone. After washing and blocking with 2% bovine serum albumin the sections were incubated with primary mouse anti-Gal-3, primary rabbit anti-NLRP3 and primary rabbit anti-IL-1β (Abcam, Cambridge, UK) antibody. Staining was visualized by using rabbit specific HRP/AEC detection IHC Kit (Abcam, Cambridge, UK) for NLRP3 and IL-1β and EXPOSE mouse and rabbit specific HRP/DAB detection IHC Kit (Abcam, Cambridge, UK). Sections were photomicrographed with a digital camera mounted on light microscope (Olympus BX51, Japan) and analyzed (15 (link)). Analysis was performed on 10 fields/section (×40). Results are presented as percent of positive staining cells per infiltrate.

The paraffin-embedded tumor samples were cut into 2 µm thick sections from the middle of each block. Four sections were stained using hematoxylin and eosin (H&E) according to standard histochemical procedures. Four sections were used for immunohistochemical determination of granzyme B-positive cells by staining with a granzyme B antibody (ab4059, Abcam, Cambridge, UK) at a 1:1250, and IL-12 and TNFα positive cells by staining with anti-IL12A antibody (ab203031, Abcam) at the dilution 1:1000, and anti-TNFα antibody (ab6671, Abcam) at the dilution 1:1300 overnight at 4 °C. Peroxidase-conjugated streptavidin–biotin kits (ab64261, rabbit-specific HRP/DAB detection IHC kit (ABC), EXPOSE Rabbit-specific HRP-AEC detection IHC kit, Abcam) were used as the colorogenic reagents. Representative images of histological slides were captured by a DP72 CCD camera (Olympus, Tokyo, Japan) connected to a BX-51 microscope (Olympus).