Av 400 mhz spectrometer

Manufactured by Bruker

Sourced in United States, Germany

The Bruker AV 400 MHz spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument. It is designed to analyze and characterize the molecular structure and properties of chemical compounds. The AV 400 MHz spectrometer provides accurate and reliable data for various applications in the fields of chemistry, biochemistry, and materials science.

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6538 uhd accurate mass q tof lc ms spectrometer, by Agilent Technologies (4 mentions) Hoechst 33258, by Merck Group (3 mentions) Topspin 3, by Bruker (2 mentions) Is50 ft ir spectrometer, by Specac (2 mentions) Lc 20at, by Shimadzu (2 mentions) 1200 hplc system, by Agilent Technologies (2 mentions) Uv 1800, by Shimadzu (2 mentions) Analytical hplc, by Shimadzu (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sybr gold, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Caspase 3 assay kit, by Thermo Fisher Scientific (1 mentions) Milli q gradient ultrapure water system, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Bruker (1 mentions) 4 mm hr mas dual inverse 1h 13c probe, by Bruker (1 mentions)

Spelling variants of this product

400 MHz spectrometer (382 citations) AV-400 (279 citations) AV-400 spectrometer (267 citations) 400 spectrometer (95 citations) Bruker spectrometers (71 citations) AV-400 MHz (19 citations) AV 400 MHz NMR spectrometer (10 citations) AV spectrometers (3 citations) AVANCE AV 400 (400/100 MHz) spectrometer (2 citations) AVANCE AV 400 MHz spectrometer (2 citations)

51 protocols using av 400 mhz spectrometer

Solid-State and Solution NMR Analysis of Cyclic Carbonates

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Solid-state NMR spectra were collected at room temperature using an AVANCE III 200 MHz spectrometer equipped with a MAS probe. The spectra were recorded using cross polarization with high power decoupling, with samples spinning at 4.5 kHz. The number of scans was in the range of 6000–8000. The contact time and recycling delays were set at 10 ms and 2 s for ¹³C NMR respectively. For ²⁹Si NMR, the contact time and recycling delays were set at 2 ms and 1.5 s.
For solution NMR measurements, ¹³C and ¹H NMR spectra were obtained using a Bruker AV 400 MHz spectrometer. Chemical shifts are reported in ppm relative to deuterated chloroform.
Where separation was needed to isolate the cyclic carbonate prior to NMR analysis, silica gel flash chromatography was used with a 3 : 1 ethylacetate : ether mixture as solvent.

Kolle J.M, & Sayari A. (2019). Novel porous organocatalysts for cycloaddition of CO2 and epoxides. RSC Advances, 9(42), 24527-24538.

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Detailed Analytical Methods for Novel Compounds

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DMEM, FBS, L-glutamine, penicillin-streptomycin and SYBR Gold (molecular biology grade) were procured from Invitrogen. Caspase-3 assay kit was procured from Thermo Scientific. All single stranded oligonucleotide sequences (Table S1) and the primers for RT-PCR (Table S2) were purchased from Integrated DNA technologies (IDT). DMSO, MTT, Hoechst 33258 and DAPI were obtained from Sigma Aldrich (Merck). All other chemicals were of analytical reagent grade and used without further purification unless otherwise stated. Ultrapure water (double-distilled) obtained from Milli-Q Gradient ultrapure water system (Millipore) and was used in all experiments. ¹H and ¹³C NMR spectra were recorded on Bruker AV-400 MHz spectrometer with chemical shifts reported as parts per million (ppm) (in DMSO-d6, tetramethylsilane as an internal standard) at 20 °C. UV-vis absorption and emission spectra were measured in quartz cuvettes of 1 cm path length. HRMS were obtained on Agilent Technologies 6538 UHD Accurate-Mass Q-TOF LC/MS spectrometer. HPLC traces were obtained from Shimadzu analytical HPLC.

Suseela Y.V., Satha P., Murugan N.A, & Govindaraju T. (2020). Recognition of G-quadruplex topology through hybrid binding with implications in cancer theranostics. Theranostics, 10(23), 10394-10414.

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Synthesis and Evaluation of Antifungal Compounds

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Reagents and solvents used without further purification are analytically or chemically pure. Melting points (m.p.) were determined on an uncorrected WRS-1B digital melting point apparatus (Shanghai Precision and Scientific Instrument Corporation, China). The FT-IR spectra were recorded on a Thermo Nicolet 380 FT-IR spectrometer (Thermo Nicolet Corporation, America). ¹H NMR, ¹³C NMR, and ¹H-¹H NOESY spectra were collected on a Bruker AV 400 MHz spectrometer (Bruker Corporation, Germany) at room temperature with DMSO-d₆ as a solvent. Mass spectra were recorded on a TRACE 2000 spectrometer (Finnigan Corporation, America). Elemental analyses were determined on an Elementar Vario EL cube analyzer (Elementar Corporation, German). Reactions were monitored by thin layer chromatography (TLC) on silica gel GF₂₄₅ (400 mesh). The tested strains Fg, Rs, Bc and Cc were provided by the Laboratory of Plant Disease Control at Nanjing Agricultural University.

Wang X., Ren Z., Wang M., Chen M., Lu A., Si W, & Yang C. (2018). Design and synthesis of novel 3-(thiophen-2-yl)-1,5-dihydro-2H-pyrrol-2-one derivatives bearing a hydrazone moiety as potential fungicides. Chemistry Central Journal, 12, 83.

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Characterization of Lignin by FTIR and NMR

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2 mg of EHRL sample was mixed with 70 mg of KBr and prepared in the form of pellets at 1 MPa for Fourier transform infrared spectroscopy (FTIR) analysis. FTIR spectra of EHRL were recorded with a NEXUS 670 spectrometer (Thermo Nicolet Corp., Madison, WI). ¹³C NMR spectra were recorded with a Bruker AV 400 MHz spectrometer at 25 °C in DMSO-d6. For ¹³C NMR experiments, 140 mg of lignin was dissolved in 0.5 ml DMSO-d6. The spectra were recorded in FT mode at 100.6 MHz. The inverse-gated-decoupling sequence was used with the parameters of 2 s relaxation delay, 1.4 s acquisition time, 64 K data points, 30° pulse angle, and 30.000 scans.

Yan K., Liu F., Chen Q., Ke M., Huang X., Hu W., Zhou B., Zhang X, & Yu H. (2016). Pyrolysis characteristics and kinetics of lignin derived from enzymatic hydrolysis residue of bamboo pretreated with white-rot fungus. Biotechnology for Biofuels, 9, 76.

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HR-MAS NMR of Biological Samples

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A Bruker AV-400 MHz spectrometer operating at a resonance frequency of 399.427 MHz was used for the HR-MAS NMR experiments with a 4 mm HR-MAS dual inverse ¹H/¹³C probe with magic angle gradient. A spinning frequency of 4 kHz and a temperature of 277 K was used during data acquisition.
A rotor synchronized Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence with water suppression was used to obtain one-dimensional ¹H HR-MAS spectra [19 (link)]. The one-dimensional spectra were collected applying 256 transients, a spectral width of 8000 Hz, data array size of 16K points, an acquisition time of 2 seconds and a relaxation delay of 2 seconds. The echo time applied for CPMG-spectra was 6.4 ms (8 loops, rotor-synchronized inter-pulse delay of 0.8 ms). The free induction decays (FIDs) were exponentially weighted with a line broadening of 1 Hz. TOPSPIN 3.5 (Bruker BioSpin, Germany) was used to phase the spectra manually and to perform automatically baseline correction. Data deposit publicly available.

Augustijn D., de Groot H.J, & Alia A. (2019). A robust circadian rhythm of metabolites in Arabidopsis thaliana mutants with enhanced growth characteristics. PLoS ONE, 14(6), e0218219.

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Comprehensive Characterization of Polyazine Compounds

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The FT-IR spectra were recorded in KBr pellets in the region 400–4000 cm⁻¹ using Perkin Elmer FT-IR 8000 spectrophotometer. UV–visible spectra were recorded in DMSO solution with Systronics double beam UV–visible spectrophotometer 2202 in the range 200–800 nm. ¹H-NMR and ¹³C-NMR spectra were recorded on Bruker AV400 MHZ spectrometer by using DMSO-d₆ as a solvent. TG-DTA measurement was made in NETZSCH STA 409 PC thermal analysis equipment between 30 °C and 800 °C (in N₂; rate, 10 °C/min). The molecular weight of polyazine was determined by gel permeation chromatography (GPC) using Polystyrene standard and eluted in Dimethylacetamide (DMAC) at a flow rate of 0.5 ml/min at 25 °C on a Water Alliance GPC model (GPC, Water 515 HPLC) fitted with water 2414 Refractive index detector and Styragel HMW 6E DMF Column. The surface morphology of azine and polyazine was monitored using JEOL Model JSM – 6390LV microscope.

Dineshkumar S, & Muthusamy A. (2016). Investigation of aggregation induced emission in 4-hydroxy-3-methoxybenzaldehyde azine and polyazine towards application in (opto) electronics: synthesis, characterization, photophysical and electrical properties. Designed Monomers and Polymers, 20(1), 234-249.

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Analytical Characterization of Compounds

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All reagents for synthesis were analytically pure. All the solvents for spectroscopic measurement were chromatographically pure. ¹H NMR spectra were recorded at 400 MHz, in CDCl₃ solution on a Bruker AV400 MHz spectrometer and chemical shifts were recorded in parts per million (ppm) with TMS as the internal reference. Mass spectra (MS) were obtained on a QTRAP LC/MS/MS system (API2000; Applied Biosystems, Foster City, CA, USA), and signals were given in m/z. Fluorescence spectra were determined with a Hitachi F-4600 fluorescence spectrophotometer. Photoluminescence (PL) quantum yields were determined using a Hamamatsu system for absolute PL quantum yield measurements (type C11347).

Meng X., You L., Li S., Sun Q., Luo X., He H., Wang J, & Zhao F. (2020). An ICT-based fluorescence enhancement probe for detection of Sn2+ in cancer cells. RSC Advances, 10(62), 37735-37742.

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Synthesis and Characterization of Phosphinoborane 1

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All manipulations were carried out under a dry argon atmosphere by
using flame-dried Schlenk-type glassware on a vacuum line or in a
glovebox. Solvents were dried by standard procedures over Na(K)/K/Na/benzophenone
and distilled under argon. One-dimensional (³¹P, ¹³C, ¹¹B, and ¹H) and two-dimensional NMR spectra
in a C₆D₆ or toluene-d₈ solution were recorded on a Bruker AV400 MHz spectrometer
(external standard TMS for ¹H and ¹³C; 85% H₃PO₄ for ³¹P) at ambient temperature.
Reaction progress was monitored by ³¹P{¹H} and ¹¹B NMR spectra of reaction mixtures. The FTIR spectra of crystalline
products were recorded using a Nicolet iS50 FT-IR spectrometer equipped
with the Specac Quest single-reflection diamond attenuated total reflectance
(ATR) accessory, and spectral analysis was carried out by using the
OMNIC software package. Elemental analyses were performed at the University
of Gdańsk using a Vario El Cube CHNS apparatus. Crystallographic
analyses were performed on a STOE IPDS II diffractometer using Mo
Kα radiation (λ = 0.71073 Å). Phosphinoborane 1 was synthesized via the procedure described in ref (19 (link)).

Szynkiewicz N., Chojnacki J, & Grubba R. (2020). Activation of N2O and SO2 by the P–B Bond System. Reversible Binding of SO2 by the P–O–B Geminal Frustrated Lewis Pair. Inorganic Chemistry, 59(9), 6332-6337.

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Characterization of NCAs and AMPs

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¹H nuclear magnetic resonance spectroscopy (¹H-NMR) was carried out on a Bruker AV, 400 MHz spectrometer (Bruker Corporation, Billerica, MA, USA) to characterise the molecular structures of NCAs, AMPs and all intermediate products. Fourier transform infrared spectroscopy was performed on a Bruker Vertex 70 (Bruker Corporation) to ascertain the conversion rate of monomers. Gel permeation chromatography was performed on a system equipped with an isocratic pump (Model 1100, Agilent Technology, Santa Clara, CA, USA), a Dawn Heleos multi-angle laser light scattering detector (Wyatt Technology, Santa Barbara, CA, USA), and an Optilab rEX refractive index detector (Wyatt Technology). The detection wavelength of the laser light scattering detector was 658 nm. Separations were performed using serially-connected size-exclusion columns (100 Å, 500 Å, 1 × 10³ Å and 1 × 10⁴ Å Phenogel columns, 5 μm, 300 × 7.8 mm, Phenomenex Inc., Torrance, CA, USA) at 60°C using N, N-dimethylformamide containing 0.05 M LiBr as the eluent phase at a flow rate of 1.0 mL/min. MALDI-TOF MS was performed on a Bruker Daltonics FlexAnalysis system (Bruker Corporation) to measure the molecular weight of AMPs.

Wang X., Li Q, & Yang H. (2023). Effect of radiation sterilisation on the structure and antibacterial properties of antimicrobial peptides. Biomaterials Translational, 4(1), 51-61.

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Comprehensive Characterization of Lignite Subfraction

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The elemental
analysis was performed at the mode of CHNS using a Vario EL III elementary
analyzer. The SL lignite and SDP subfractions were characterized by
FTIR spectroscopy using a PE-Spectrum One IR spectrometer at ambient
temperature. In the FTIR measurements, the sample was mixed with KBr
at a mass ratio of 1:100 and the mixture was pressed into a pellet.
GPC analysis was carried out on Shimadzu LC-20AT high-performance
liquid chromatography (HPLC) with a Shimpack GPC-8025 (300 mm length,
0.8 cm i.d.) separation column isothermally at 25 °C. THF was
used as the mobile phase with a flow rate of 1.2 mL·min^–1. Synchronous fluorescence spectra were recorded on a Hitachi F-4600
spectrophotometer with a 150 W xenon lamp as the excitation source.
The difference between excitation and emission wavelength was 14 nm.
The spectral measurement at room temperature was made with the use
of a quartz cell of a 1 cm path length. The samples were dissolved
in THF, and the concentration was 5 μg·mL^–1. A 10 mg solid sample (THFS) was dissolved in 0.6 mL of DMSO-d₆ and added with a few drops of TMS as an internal
reference. The solution was then subjected to ¹H-NMR and ¹³C-NMR analysis, which were recorded on a Bruker AV 400 MHz
spectrometer at 25 °C.

Liu M., Lei Z., Cao X., Yan J., Shui H., Wang Z., Hu J, & Hong M. (2023). Construction of Macromolecules of Depolymerized Lignite. ACS Omega, 8(25), 22820-22826.

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