Sc 1207

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-1207 is a laboratory equipment designed for sample preparation and analysis. It is a centrifuge tube that can be used for a variety of applications, including cell separation, protein purification, and nucleic acid extraction. The product specifications and technical details are available upon request.

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Lab products found in correlation

5 protocols using sc 1207

Immunoblotting and Immunoprecipitation Protocols

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Immunoblots were performed as previously described [5 (link)]. Antibodies against p16 (1:200 dilution; #sc-1207), ALDH2 (1:200 dilution; #sc-100496) and acetylated FoxO1 (1:200 dilution; #sc-49437) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against p53 (1:1000 dilution; #2524), LC3B (1:1000 dilution; #3868), SirT1 (1:1000 dilution; #3931), acetyl-lysine (1:1000 dilution; #9441), Atg7 (1:1000 dilution; #8558), Rab7 (1:1000 dilution; #9367) and FoxO1 (1:1000 dilution; #2880) were purchased from Cell Signaling Technology. Antibody against DNPH (1:2000 dilution; #ab93160), LAMP2 (1:1000 dilution; #ab25339), Tubulin (1:1000 dilution; #ab179513), GAPDH (1:10000 dilution; #ab181602) and TBP (1:5000 dilution; #ab28175) were purchased from Abcam. Antibody binding was detected via enhanced chemiluminescence (Millipore) and scanned with ChemiDocXRS (Bio-Rad Laboratory, Hercules, CA). Immunoblot band intensity was analyzed with Lab Image software. For immunoprecipitation analysis, lystes were mixed with primary antibody. Immunocomplexes were separated by SDS-PAGE and detected with western blot analyses.

Wu B., Yu L., Wang Y., Wang H., Li C., Yin Y., Yang J., Wang Z., Zheng Q, & Ma H. (2016). Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1. Oncotarget, 7(3), 2175-2188.

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Immunohistochemical Profiling of Prostate Cancer

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The primary antibodies used and their subsequent dilutions were: anti-P16 (rabbit, 1:1000, SC-1207, Santa Cruz), anti-human AR (mouse, 1:250, sc-7305, Santa Cruz Biotechnology), anti-CK5 (rabbit, 2400, PRB-160P, Covance), anti-CK8 (mouse, 1:2000, MMS-162P, Covance), anti-p63 (mouse, 1:2000, sc-8431, Santa Cruz), anti Ki67 (mouse, 1:1000, NCL-ki67, Novacastra), anti-E-cadherin (mouse, 1:200, Cat. No. c20820, BD Transduction Laboratoriesc, Sparks, MD, United States), anti-mouse/ human androgen receptor (rabbit, 1:250, sc-816, Santa Cruz), anti-synaptophysin (rabbit, 1:100, Cat. No. 18–0130, Invitrogen), anti-SPP1 (rabbit, 1:200, Cat. No. 91655, Abcam, Cambridge, MA, USA), CD44 (Rat, 1:50, sc-18849, Santa Cruz) and anti-Vimentin (Chicken, 1:2000, Cat. No. 919101, Biolegend). The biotinylated anti-rabbit or anti-mouse secondary antibody (BA-1000 or BA-9200, Vector Laboratories), or anti-rabbit or anti-mouse conjugated to AlexaFluor488 or to AlexaFluor594 (Molecular Probes) secondary antibody that were used for IHC or IF staining, respectively.

Lee D.H., Yu E.J., Aldahl J., Yang J., He Y., Hooker E., Le V., Mi J., Olson A., Wu H., Geradts J., Xiao G.Q., Gonzalgo M.L., Cardiff R.D, & Sun Z. (2019). Deletion of the p16INK4a tumor suppressor and expression of the androgen receptor induce sarcomatoid carcinomas with signet ring cells in the mouse prostate. PLoS ONE, 14(1), e0211153.

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Immunohistochemical Analysis of Cell Markers

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Staining and image capture were performed on 3μm formalin-fixed and paraffin-embedded sections as described(15 (link),20 (link)). Primary antibodies used included mouse monoclonal antibodies against hemagglutinin tag (HA, MMS-101P, Covance, 1:500), p21 (cat#sc-6246 Santa Cruz, 1:200), gamma-H2AX (cat#05-636 Millipore, 1:500), and pATM (cat#200-301-500 Rockland, 1:200), as well as rabbit polyclonal antibodies against cleaved caspase 3 (CC3, cat#9661S Cell Signaling Technology, 1:200), Ki67 (cat#NCL-Ki67P Novocastra, 1:200), phospho-histone 3 (pH3, cat#06-570 Millipore, 1:200), macroH2A (cat#ab37264 Abcam, 1:150), p16 (cat#sc-1207 Santa Cruz, 1:200), and p53 (cat#NCL-p53-CM5p Novacastra, 1:1000). Cell counting was achieved using Image J software as well as Adobe Photoshop.

Sinha V.C., Qin L, & Li Y. (2014). A p53/ARF-dependent Anticancer Barrier Activates Senescence and Blocks Tumorigenesis without Impacting Apoptosis. Molecular cancer research : MCR, 13(2), 231-238.

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Protein Expression and Immunoblotting Protocol

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Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM sodium chloride, 1% (v/v) NP-40, 0.5% (w/v) sodium deoxycholate, 0.1% (w/v) SDS, 5mM sodium fluoride, 1 mM sodium orthovanadate, 1 mM PMSF, 1:100 Protease Inhibitor Cocktail (Sigma-Aldrich)) for protein extraction. Proteins were run on 8–12% acrylamide gels, transferred to nitrocellulose membranes and visualized by immunoblotting with the following antibodies anti-ß-ACTIN (1:2000, A2228, Sigma-Aldrich), anti-H-RAS (1:1000, sc-520, Santa Cruz Biotechnology Inc.), anti-MYC (1:1000, AB32072, Epitomics (Abcam)), anti-p16 (1:1000, sc-1207, Santa Cruz Biotechnology Inc.), anti-p19 (1:1000, sc-32748, Santa Cruz Biotechnology Inc.), anti-p53 (1:500, NCL-p53-CM5p, Novocastra), anti-PTEN (1:1000, sc-7974, Santa Cruz Biotechnology Inc.), anti-TSC2 (1:1000, 3990s, Cell signaling) and anti-VINCULIN (1:5000, ab129002, Abcam).

Brandt L.P., Albers J., Hejhal T., Pfundstein S., Gonçalves A.F., Catalano A., Wild P.J, & Frew I.J. (2018). Mouse genetic background influences whether HrasG12V expression plus Cdkn2a knockdown causes angiosarcoma or undifferentiated pleomorphic sarcoma. Oncotarget, 9(28), 19753-19766.

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Immunohistochemical Analysis of Cell Markers

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Immunohistochemical analysis was performed on paraffin embedded sections by indirect immunohistochemistry procedure using rabbit anti-p16 (sc-1207, Santa Cruz Biotechnology, Dallas, TX, USA), anti-p21 (ab109199, Abcam plc, Cambridge, UK), anti-HA (3724, Cell Signaling Technology, Beverly, MA, USA), anti-CD34 (ab81289, abcam), goat anti-collagen IV (1340-01, SouthernBiotech, Birmingham, AL, USA) and sheep anti-nephrin (AF4269, R&D Systems, Minneapolis, MN, USA) antibodies. For p16, p21 and collagen IV immunostaining, sections were pretreated by proteinase K (19131, QIAGEN K.K., Tokyo, Japan). For the other antigens, sections were pretreated by citrate buffer (pH 6.0). Following the first antibody, sections were incubated with Alexa Fluor 488 or 594-conjugated donkey anti-rabbit antibody (A21206, A21207,

Ueda S., Tominaga T., Ochi A., Sakurai A., Nishimura K., Shibata E., Wakino S., Tamaki M., & Nagai K. (2021). TGF-β1 is Involved in Senescence-Related Pathways in Glomerular Endothelial Cells by p16 Translocation and p21 Induction.

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