Punches were blocked in IF buffer (1% BSA and 0.05% Tween‐20 in PBS) for 1–2 h at RT, followed by the incubation with primary antibody diluted in IF buffer, for 24 h at 4°C. Punches were washed in 1× PBS for 1–2 h and incubated for 24 h at 4°C with secondary antibody and DAPI (ThermoFisher; D1306) diluted 1:10 000 in IF buffer. The following primary antibodies were used for immunostaining of expanded samples: mouse anti‐acetylated tubulin (Sigma, T7451) at 1:4000, mouse anti‐alpha tubulin (Sigma; T6074) at 1:500, rabbit antibeta tubulin (Abcam; ab15568) at 1:250, rabbit anti‐ARL13B (Abcam; ab83879) at 1:150, rabbit anti‐Cep164 (Proteintech; 22227‐1‐AP) at 1:500, rabbit anti‐ANKRD26 (GeneTex; GTX128255) at 1:200, rabbit anti‐FBF1 (sigma; HPA023677) at 1:100, rabbit anti‐RPGRIP1L (Proteintech; 55160‐1‐AP) at 1:150, rabbit anti‐IFT88 (Proteintech; 13967‐1‐AP) at 1:150, mouse anti‐Rootletin (Santa Cruz; sc‐374056) at 1:50, rabbit antipericentrin (Abcam; ab4448) at 1:400, rabbit anti‐Cep290 (Abcam; ab84870) at 1:150 and rabbit antipolyglutamylated tubulin (AdipoGen; AG‐25B‐0030) at 1:800. Secondary antibodies antimouse Alexa Fluor 488 (Invitrogen; A11029), antirabbit Alexa Fluor 488 (Invitrogen; A11034), antimouse Alexa Fluor 555 (Invitrogen; A28180), antirabbit Alexa Fluor 555 (Invitrogen; A21429) were used at a 1:800 dilution to label primary antibodies.
Ag 25b 0030
Manufactured by Adipogen
AG‐25B‐0030 is a laboratory equipment product. It is a device designed for use in research and scientific applications. The core function of this product is to perform a specific task or function within a laboratory setting. No further details about the intended use or other characteristics of this product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
D1306, by Thermo Fisher Scientific (1 mentions)
A11029, by Thermo Fisher Scientific (1 mentions)
A11034, by Thermo Fisher Scientific (1 mentions)
A21429, by Thermo Fisher Scientific (1 mentions)
T6074, by Merck Group (1 mentions)
A28180, by Thermo Fisher Scientific (1 mentions)
Ab15568, by Abcam (1 mentions)
Ab4448, by Abcam (1 mentions)
Ab84870, by Abcam (1 mentions)
T7451, by Merck Group (1 mentions)
A11011, by Thermo Fisher Scientific (1 mentions)
A32723, by Thermo Fisher Scientific (1 mentions)
T6793, by Merck Group (1 mentions)
Ag 20b 0020, by Adipogen (1 mentions)
Bs 40296g hrp, by Bioss Antibodies (1 mentions)
2 protocols using ag 25b 0030
1
Immunostaining of Expanded Samples
2
Antibody Usage for Centrosomal Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following primary antibodies were used: anti-CP110 (Proteintech, 2780–1-AP, 1:2000 for western blotting, 1:500 for immunostaining), anti-CEP97 (Proteintech, 22,050–1-AP, 1:1000), anti-turboGFP (Origene, TA150041, 1:2000 for western blotting or Proteintech, tbfms, 1:500 for immunostaining), anti-myc-tag (Invitrogen, MA1-21,316, 1:2000 for western blotting, 1:500 for immunostaining), anti-His-tag (Immunoway, YM3004, 1:2000); anti-acetylated α-tubulin (Sigma, T6793, 1:500), anti-γ-tubulin (Sigma, T6557, 1:500), EP1332Y (Abcam, ab52866, 1:4000), GT335 (Adipogen, AG-20B-0020, 1:4000 for western blotting, 1:500 for immunostaining), PolyE (Adipogen, AG-25B-0030, 1:4000 for western blotting, 1:500 for immunostaining), anti-ARL13B (Proteintech, 17,711–1-AP, 1:500), anti-β-actin (Sungene biotech, KR9001T, 1:4000). The secondary antibodies were goat-anti-mouse or donkey-anti-rabbit antibodies coupled with horseadish peroxidase (Bioss, bs-40296G-HRP and bs-0295D-HRP 1:4000) for western blotting or those coupled with Alexa Fluor 488 or 568 for immunofluorescence analysis (Invitrogen, A32723 and A-11011 1:750).
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