H2DCFDA (Cat. number: HY-D0940), BrdU (Cat. number: HY-15910), palbociclib (Cat. number: HY-50767), ribociclib (Cat. number: HY-15777), niraparib (Cat. number: HY-10619), and olaparib (Cat. number: HY-10162) were from MedChem Express. Anti-phospho-γ-H2AX (Ser139) (Cat. number: 05-636) and FITC-conjugated anti-BrdU (Cat. number: MAB3262F) antibodies were from Millipore. Anti-Rb (ab181616), Anti-phospho-Rb (Ser780) (ab173289), Anti-histone H3 (ab1791), and Anti- PARP1 (ab191217) were from Abcam.
Anti histone h3 ab1791
Manufactured by Abcam
Sourced in United States
Anti-Histone H3 (ab1791) is a primary antibody that specifically binds to histone H3, a core component of nucleosomes in eukaryotic cells. This antibody is suitable for use in various immunodetection techniques to identify and quantify histone H3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag, by Merck Group (2 mentions)
Anti phospho γ h2ax ser139, by Merck Group (1 mentions)
Ribociclib, by MedChemExpress (1 mentions)
Niraparib, by MedChemExpress (1 mentions)
H2dcfda, by MedChemExpress (1 mentions)
Bromodeoxyuridine (brdu), by MedChemExpress (1 mentions)
Palbociclib, by MedChemExpress (1 mentions)
Olaparib, by MedChemExpress (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Anti h3k9me3 antibody, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase, by Cell Signaling Technology (1 mentions)
Anti lamin a c sc 376248, by Santa Cruz Biotechnology (1 mentions)
Anti β actin sc 4778, by Santa Cruz Biotechnology (1 mentions)
Anti polr2c 1y26, by Abcam (1 mentions)
Anti v5, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti ha hrp, by Santa Cruz Biotechnology (1 mentions)
Anti flag hrp, by Merck Group (1 mentions)
Anti gst, by GE Healthcare (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Cytiva (1 mentions)
18 protocols using anti histone h3 ab1791
1
DNA Damage and Cell Cycle Assay
2
Generation and Validation of Anti-HP1BP3 Antibody
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The anti-HP1BP3 antibody was created by injecting guinea pigs with the peptide STRETPPKSKLAEGEEEKPEPD-C corresponding to amino acids 47–68 of the murine protein. Peptide synthesis, keyhole limpet hemocyanin (KLH) conjugation and immunization of guinea pigs were carried out by Peptide Specialty Laboratories GmbH, Heidelberg, Germany. Anti-Histone H3 (ab1791) and anti-HP1α (ab77256) antibodies were from Abcam. Anti H3K9me3 antibody was from Cell signaling (#9754) and anti-Tubulin was purchased from Sigma-Aldrich (T5168).
3
Western Blot Analysis of Endometrial Cancer Cells
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Endometrial cancer cells were lysed in a RIPA buffer (50 mM Tris HCl pH 8, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM PMSF). Concentrations of protein were determined using the Lowry method. Proteins of the cell lysates were resolved by 8% SDS-PAGE and transferred to Immobilon P membranes. The blots were incubated for two hours at room temperature with the following primary antibodies: anti-OGT (#5368) (diluted 1:2000, Cell Signaling Technology, Danvers, MA, USA), anti-lamin A/C (sc-376248) (diluted 1:2000; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Histone H3 (ab1791) (diluted 1:2500; Abcam), and anti-β-actin (sc-4778) (diluted 1:5000; Santa Cruz Biotechnology, Dallas, TX, USA). After washing with TBST (Tris buffered saline with Tween-20), immunoblots were incubated 1h at room temperature with goat anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (diluted 1:5000, Cell Signaling Technology, Danvers, MA, USA).
4
Chromatin Enrichment and Western Blotting
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Chromatin-enriched fraction purification was performed according to the yChEFs procedure (32 ). Briefly, 150 ml of exponentially growing yeast cells (OD600 ∼ 0.7–0.8) were harvested, at least in triplicate. The whole final purified chromatin-enriched fraction P3 was resuspended in 20 μl of 1× Tris-Glycine SDS Sample Buffer and incubated for 5 min at 100°C, followed by spinning at 10 000 rpm for 30 s. This chromatin pellet was used for SDS-PAGE, followed by western blotting with different antibodies: anti-C-Myc (9E10 Santa Cruz Biotechnology), anti-Rpb3 (anti-POLR2C; 1Y26, Abcam), anti-Histone H3 (ab1791; Abcam) or anti-phosphoglycerate kinase, Pgk1 (459250; Invitrogen). Histone H3 was used as a control of purified chromatin and Pgk1 as a control of cytoplasmic contamination.
5
Antibody Dilutions for Immunoblotting
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The following antibodies were used for immunoblotting at the indicated dilutions: Anti-FLAG-HRP (A8592, Sigma Aldrich, 1:1250), anti-V5 (46–0705, Invitrogen, 1:5000), and anti-GST (27–4577–01, GE Healthcare, 1:2000); anti-p-Tyr-HRP (sc-7020, 1:500), anti-p85 (sc-423, 1:500), anti-Hsp56 (sc-1803, 1:1250), anti-GAPDH (sc-47724, 1:5000), and anti-HA-HRP (sc-7392, 1:500) were purchased from Santa Cruz Biotechnology; anti-ACK (07–757, 1:2000) and anti-ACK p-Tyr284 (09–142, 1:5000) were purchased from Millipore; anti-Histone H3 (ab1791, 1:5000) and anti-pTyr607 p85 (ab182651, 1:1000) were purchased from Abcam. The HRP-conjugated secondary antibodies donkey anti-mouse (sc-2318, 1:2000) and donkey anti-rabbit (sc-2317, 1:5000) were purchased from Santa Cruz Biotechnology. Anti-FLAG (F3165, Sigma Aldrich) was used for immunoprecipitation. If necessary, antibodies were diluted in PBS-0.1% Tween.
6
Western Blot Protocol for Protein Detection
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Cell extracts or immunoprecipitated proteins were diluted in SDS sample buffer and boiled for 5 minutes. Protein was separated on either 10% or 8% SDS‐Page gels and transferred to PVDF Transfer Membrane Hybond™ (Amersham Bioscience). Membranes were saturated with 5% non‐fat dry milk in PBS containing 0.1% Tween20 (PBST) for 1 hour at RT. The antibodies (anti ‐c‐MYC sc764, anti‐GFP sc9996, anti‐GST sc9996, anti‐E47 sc763, anti‐GAPDH sc‐32233 were from Santa Cruz Biotechnology; anti‐Flag F3165 was from Millipore; anti STRA8 Ab49405, anti‐SOHLH1 Ab41520 and anti‐HISTONE H3 Ab1791 were from Abcam; anti c‐KIT gently provided by Prof. S. Dolci) were diluted in PBST buffer and added to the PVDF membrane for 1 hour at RT or overnight at 4°C followed by incubation with the appropriate horseradish peroxidase‐conjugated secondary antibodies (Amersham Bioscience) for 45 minutes at RT. The STRA8 and SOHLH1 immunoprecipitated protein were detected after incubation with the peroxidase‐conjugated anti‐rabbit IgG light chain specific (Jackson ImmunoReasearch). All proteins were detected with ECL plus detection reagents (Amersham Bioscience) and visualized by chemiluminescence.
7
Chipmunk Hibernation Protein Analysis
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Nuclear extracts were prepared from the liver, kidney, heart, and lung of nonhibernating and hibernating chipmunks39 (link). Immunoblotting was performed as described previously39 (link). The anti-USF-1 (sc-229) and anti-USF-2 (sc-862) antibodies were purchased from Santa Cruz Biotechnology. The anti-HNF-4 antibodies (sc-8987 and H1415) were purchased from Santa Cruz Biotechnology and Perseus Proteomics, respectively. The anti-HNF-1 antibody (H69220) was purchased from Transduction Laboratories. The anti-acetyl-histone H3 (Lys9) (07-352) and anti-acetyl-histone H3 (Lys14) (07-353) antibodies were purchased from Millipore. The anti-histone H3 (tri methyl K4) (ab8580) and anti-histone H3 (ab1791) antibodies were purchased from Abcam. The anti-DYKDDDDK antibody (KO602) was purchased from TransGenic Inc.
8
Histone Immunoprecipitation from Cell Media
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Concentrated conditioned media from inhibitor-treated cells were subjected to histone IP using anti-histone antibody (MAB3422; mouse monoclonal; MilliporeSigma) conjugated to Dynabeads (Thermo Fisher Scientific), as described above. For each IP reaction, 40 μL of antibody-coupled beads (10 mg/mL) was mixed with 100 μL concentrated media and incubated on a roller for 1 hour at room temperature. Samples were then placed on a magnet rack and the supernatant was discarded. Pellets were washed once with PBS and resuspended in 25 μL of 2× loading buffer (Bio-Rad). Western blot was performed as described above using the primary antibody anti–histone H3 (ab1791; rabbit polyclonal; Abcam). Band intensity was quantified using Image Studio Lite software (version 5.2, LI-COR Biosciences).
9
Antibody Characterization and Applications
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Anti-H3K4me2 [ab32356; 10 μg for ChIP experiments, 1:2000 for western blotting (WB)], anti-histoneH3 (ab1791; 1:2000 for WB), goat anti-mouse IgG (ab6786), goat anti-rabbit IgG (ab6718), and rabbit anti-rat IgG (ab6730) were obtained from Abcam. Anti-Myc (14793; 1:50 for Co-IP, 1:1000 for WB) and anti-β-catenin (9587; 1:50 for Co-IP, 1:1000 for WB, 1:25 for ChIP) were obtained from Cell Signaling Technology. Anti-CVH [ab27591; 1:1000 for WB, immunohistochemistry (IHC) and fluorescence-activated cell sorting (FACS)] was obtained from Abcam.
10
Comprehensive Reagents Inventory for Cell Biology
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