Rosmarinic acid

Manufactured by Phytolab

Sourced in Germany

Rosmarinic acid is a naturally occurring compound found in various plants, including rosemary, sage, and thyme. It is a polyphenolic compound with antioxidant properties. Rosmarinic acid is commonly used in laboratory research and analysis.

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Rosmarinic (2 citations)

3 protocols using rosmarinic acid

Extraction and Mass Spectrometry Analysis of Bioactive Compounds

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All solvents (formic acid, water, acetonitrile, methanol) used for extraction and HPLC–DAD–ESI-MS and HPLC–DAD were of LC–MS and HPLC grade and were purchased from Thermo Fisher Scientific (Waltham, MA, USA). HPLC reference standards, rosmarinic acid (99%), and luteolin 7-O-glucoside (98.9%), were purchased from Phytolab (Vestenbergsgreuth, Germany). All the chemical reagents used for the CBMN assay are reported in our previous work [58 (link)] (see Supplementary Materials Section S3.1).

Stavropoulou L.S., Efthimiou I., Giova L., Manoli C., Sinou P.S., Zografidis A., Lamari F.N., Vlastos D., Dailianis S, & Antonopoulou M. (2024). Phytochemical Profile and Evaluation of the Antioxidant, Cyto-Genotoxic, and Antigenotoxic Potential of Salvia verticillata Hydromethanolic Extract. Plants, 13(5), 731.

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Quantitative Analysis of Rosemary Bioactives

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Acetonitrile, methanol, formic acid, caffeic acid, hesperidin, rutin, vitexin, C₈-C₂₀ alkane solution, and toluene were purchased from Sigma-Aldrich (St. Louis, MO, USA). Carnosic acid, carnosol, 12-O-methylCarnosic acid, rosmanol, rosmarinic acid, and betulinic acid were purchased from PhytoLab (Vestenbergsgreuth, Germany). Luteolin-4-glucoside was obtained from AASC Ltd. (Southampton, UK). Ultrapure water from MilliQ system (Millipore, Bedford, MA, USA) was used throughout the experiment. The proprietary rosemary extract rich in Carnosic acid was provided by Kemin Foods, L.C. (Des Moines, IA, USA). It was prepared from dried leaves by a proprietary acetone-based extraction.

Mena P., Cirlini M., Tassotti M., Herrlinger K.A., Dall’Asta C, & Del Rio D. (2016). Phytochemical Profiling of Flavonoids, Phenolic Acids, Terpenoids, and Volatile Fraction of a Rosemary (Rosmarinus officinalis L.) Extract. Molecules, 21(11), 1576.

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Quantification of Phytochemicals in Botanical Samples

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Methanol, Folin Ciocalteu reagent, quercitin, aluminium chloride, catechin, sulfuric acid, tannic acid, hydrochloric acid, chloroform, chlorhydric alcohol, isoamyl alcohol, acetic anhydride, glacial acetic acid and DPPH were purchased from Sigma-Aldrich, U.S.A. Gallic acid and vanillin were obtained from Merck, Germany, sodium carbonate was from Acros Organics, Belgium, and ferric chloride was purchased from Alfa Aesar, Germany.
In HPLC analysis, Fortis column was obtained from Fortis Technologies Ltd, UK. The used solvents (acetonitrile and formic acid) were purchased from Merck, Germany. Concerning the standards; caffeic acid, chlorogenic acid, ferulic acid, trans-p-coumaric acid, Gallic acid, rosmarinic acid, salicin, apigenin, quercetin, quercitrin, isoquercitrin, hyperoside, luteolin -7-O-glucoside, luteolin, kaempferol were from Phytolab, Germany and ellagic acid, salicylic acid, chicoric acid, naringenin, chrysin, myricetin were purchased from Merck, Germany.

Bouhenni H., Doukani K., Hanganu D., Doukani K., Şekeroğlu N., & Gezici S. (2021). Analysis of bioactive compounds and antioxidant activities of cultivated garlic (allium sativum l.) and red onion (allium cepa l.) in algeria. International Journal of Agriculture Environment and Food Sciences, 5(4), 550-560.

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