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Prism 4

Manufactured by IBM
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Prism 4.03 is a laboratory instrument designed for spectral analysis. It utilizes a prism-based optical system to disperse light into its constituent wavelengths, enabling the measurement and characterization of various types of radiation and spectra.

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Lab products found in correlation

Spss version 14, by IBM (1 mentions)

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Prism v.4 (3 citations)

13 protocols using prism 4

1

Analyzing Gene Expression Profiles

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All the data are presented as mean ± SD and were calculated from multiple experiments performed in quadruplicates. For the data analyses, Student's two-tailed t-test or one-way ANOVA were used to determine significant differences between treatment and control groups using the GraphPad Prism 4.03 software and SPSS 17.0 software. All the existing statistical analysis data were obtained from the cBioportal. Data with P<0.05 were considered significant.
Alwhaibi A., Kolhe R., Gao F., Cobran E.K, & Somanath P.R. (2019). Genome atlas analysis based profiling of Akt pathway genes in the early and advanced human prostate cancer. Oncoscience, 6(5-6), 317-336.
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2

Quantitative Analysis of Protein Expression

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All the data are presented as mean ± SD and were calculated from multiple independent experiments performed in quadruplicates. For normalised data analysis, data was confirmed that normality assumption was satisfied and analysed using paired sample t-test (dependent t-test) and/or further confirmed with non-parametric test and Wilcoxon signed-rank test. For all other analysis, Student’s two-tailed t-test or analysis of variance test were used to determine significant differences between treatment and control values using the GraphPad Prism 4.03 software and SPSS 17.0 software.
Gao F., Alwhaibi A., Artham S., Verma A, & Somanath P.R. (2018). Endothelial Akt1 loss promotes prostate cancer metastasis via β-catenin-regulated tight-junction protein turnover. British Journal of Cancer, 118(11), 1464-1475.
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3

Standardized Multivariate Data Analysis

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All the data are presented as Mean + SD and were calculated from multiple independent experiments performed in quadruplicates. For normalized data analysis, data was confirmed that normality assumption was satisfied and analyzed using paired sample t test (dependent t test) and/or further confirmed with non-parametric test Wilcoxon signed rank test. For all other analysis, Student’s two-tailed t test or ANOVA test were used to determine significant differences between treatment and control values using the GraphPad Prism 4.03 software and SPSS 17.0 software.
Gao F., Artham S., Sabbineni H., Al-Azayzih A., Peng X.D., Hay N., Adams R.H., Byzova T.V, & Somanath P.R. (2016). Akt1 promotes stimuli-induced endothelial-barrier protection through FoxO-mediated tight-junction protein turnover. Cellular and molecular life sciences : CMLS, 73(20), 3917-3933.
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4

Quantitative Analysis of Protein Expression

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All the data are presented as Mean ± SD and were calculated from multiple independent experiments performed in quadruplicates. For normalized data analysis, data was confirmed that normality assumption was satisfied and analyzed using paired sample t-test (dependent t-test) and/or further confirmed with non-parametric test Wilcoxon signed rank test. For all other analysis, Student’s two-tailed t-test or ANOVA test were used to determine significant differences between treatment and control values using the GraphPad Prism 4.03 and SPSS 17.0 software.
Gao F., Sabbineni H., Artham S, & Somanath P.R. (2017). Modulation of long-term endothelial-barrier integrity is conditional to the cross-talk between Akt and Src signaling. Journal of cellular physiology, 232(10), 2599-2609.
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5

Detailed Quantitative Analysis Protocol

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All the data are presented as Mean ± SD and were calculated from multiple independent experiments performed in quadruplicates. For normalized data analysis, data was confirmed that normality assumption was satisfied and analyzed using paired sample t-test (dependent t-test) and/or further confirmed with non-parametric test Wilcoxon signed rank test. For all other analysis, Student’s two-tailed t-test or ANOVA test were used to determine significant differences between treatment and control values using the GraphPad Prism 4.03 and SPSS 17.0 software.
Sabbineni H., Verma A, & Somanath P.R. (2018). Isoform-Specific Effects of Transforming Growth Factor-β on Endothelial to Mesenchymal Transition. Journal of cellular physiology, 233(11), 8418-8428.
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6

Comprehensive Bioinformatics Data Analysis

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All the data are presented as mean ± SD and were calculated from multiple independent experiments performed in quadruplicates. For normalized data analysis, data was confirmed that normality assumption was satisfied and analyzed using paired sample t-test (dependent t-test) and/or further confirmed with non-parametric test Wilcoxon signed rank test. For all other analyses, Student’s two-tailed t-test or ANOVA test were used to determine significant differences between treatment and control values using the GraphPad Prism 4.03 software and SPSS 17.0 software. Data with P<0.05 were considered significant.
Gao F., Alwhaibi A., Sabbineni H., Verma A., Eldahshan W, & Somanath P.R. (2017). Suppression of Akt1- β-catenin pathway in advanced prostate cancer promotes TGFβ1-mediated epithelial to mesenchymal transition and metastasis. Cancer letters, 402, 177-189.
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7

Comprehensive Protein Quantification Assay

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All the data are presented as Mean ± SD and were calculated from multiple independent experiments performed in quadruplicates. For all the analyses, Student’s two-tailed t-test or ANOVA parametric test (equal sample size) was used to determine significant differences between treatment and control values using the GraphPad Prism 4.03 and SPSS 17.0 software.
Alharthi A., Verma A., Sabbineni H., Adil M.S, & Somanath P.R. (2020). Distinct effects of pharmacological inhibition of stromelysin1 on endothelial-to-mesenchymal transition and myofibroblast differentiation. Journal of cellular physiology, 236(7), 5147-5161.
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8

Detailed Statistical Analysis of Biological Data

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All the data are presented as Mean ± SD and were calculated from multiple independent experiments performed in quadruplicates. For normalized data analysis, data was confirmed that normality assumption was satisfied and analyzed using paired sample t-test (dependent t-test) and/or further confirmed with non-parametric test Wilcoxon signed rank test. For all other analysis, Student’s two-tailed t-test or ANOVA test were used to determine significant differences between treatment and control values using the GraphPad Prism 4.03 software and SPSS 17.0 software.
Sabbineni H., Verma A., Artham S., Anderson D., Amaka O., Liu F., Narayanan S.P, & Somanath P.R. (2019). Pharmacological inhibition of β-catenin prevents EndMT in vitro and vascular remodeling in vivo resulting from endothelial Akt1 suppression. Biochemical pharmacology, 164, 205-215.
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9

Statistical Analysis of Experimental Data

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Descriptive statistical analyzes(i.e., mean and standard deviation) and student's unpaired t-test were carried out. The analysis was performed using Windows Graph pad, Prism 4 software, and SPSS version 14.0 software (SPSS Inc., Chicago, Illinois, USA).
Gandhi R., Bhowate R., Nayyar A.S., Gandhi S, & Dongarwar G. (2016). Color Doppler-ultrasonography in oral squamous cell carcinoma: Making ultrasonography more meaningful. Advanced Biomedical Research, 5, 29.
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10

Statistical Analysis of Experimental Data

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The data were presented as the means ± SEM. A one-way ANOVA with Tukey's post hoc test or least significance difference (LSD) test was used to determine statistical significance. P < 0.05 was set as the cutoff for statistical significance. The statistical analyses were performed using GraphPad Prism 4 software and SPSS 18.
Sun J., Zhou H., Bai F., Ren Q, & Zhang Z. (2016). Myelin injury induces axonal transport impairment but not AD-like pathology in the hippocampus of cuprizone-fed mice. Oncotarget, 7(21), 30003-30017.
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