where Yij = observation; µ = overall mean; and Di = effect of diet; ɛij = residual error.
The assumption of normality was checked using the Shapiro–Wilk test. Levene’s homogeneity of variance test was used to assess homoscedasticity. If such an assumption did not hold, the Brown–Forsythe statistic was applied to test the equality of group means instead of the F one. Pairwise multiple comparisons were performed to test the difference between each pair of means (Tukey’s test and Tamhane’s T2 in the case of assumed or not assumed equal variances, respectively).
Met metabolism and the gene expression data were analyzed by one-way ANOVA using GraphPad Prism 8 software (San Diego, CA, USA). If there were significant differences between dietary groups, Tukey’s test was applied as post hoc analysis.
The results were expressed as the mean and pooled standard error of the mean (SEM). Significance was set at p ≤ 0.05.