Bbltm mueller hinton broth

For the synthesis of the microgels: N-isopropylacrylamide (NIPAM, 99%) and 2-(dimethylamino)ethyl methacrylate (DMAEMA, 98%, Aldrich, St. Quentin Fallavier, France) were used as monomers. N,N’-Methylenbis(acrylamide) (MBA, Aldrich), was used as the crosslinking agent and ammonium persulfate (APS, Aldrich) was the initiator of the reaction. Iodomethane (Aldrich, 99%) and 1-iodobutane (Aldrich, 99%) were employed as the alkylating agent. Anhydrous dimethylformamide (DMF) (99.5% purity) and hexane (95%) was supplied by Sigma Aldrich, as used as received.
For the microbiological assays: Sodium chloride (NaCl, 0.9%, BioXtra, Steinheim, Germany, suitable for cell cultures) and phosphate buffered saline (PBS, pH 7.4) were purchased from Aldrich. The microbial growth media, BBL^TM Mueller Hinton broth was obtained from Becton, Dickinson and Company (Madrid, Spain). Sheep blood (5%) Columbia Agar plates were acquired from BioMérieux (Madrid, Spain). Gram-positive Staphylococcus aureus (S. aureus, ATCC 29213) and Staphylococcus epidermidis (S. epidermidis, ATCC 12228), were used as bacterial strains, and the yeast Candida parapsilosis (C. parapsilosis, ATCC 22109) was used as fungal strain and purchased from Oxoid (Wesel, Germany).

The monomer 2-(4-methylthiazol-5-yl)ethyl methacrylate (MTA) was synthesized as previously reported [20 (link)]. The monomer 2-hydroxyethyl methacrylate (HEMA, 99%; Aldrich, Steinheim, Germany) was distilled prior to use. 2,2′-Azobisisobutyronitrile (AIBN, 98%; Acros, Buch, Switzerland) was recrystallized twice from methanol (MeOH, 99.9%; Aldrich) prior to use. Anhydrous dimethyl sulfoxide (DMSO, 99.8%) and N,N-dimethylformamide (DMF, 99.8%) were purchased from Alfa-Aesar (Karlsruhe, Germany) and were used as received. 1-Iodobutane (BuI, 99%, Aldrich), iodomethane (MeI, 99%; Aldrich), and hexane (96%; Scharlau, Sentmenat, Spain) were used as received.
For the microbiological assays, sodium chloride (NaCl, 0.9%, BioXtra, Steinheim, Germany, suitable for cell cultures) and phosphate buffered saline (PBS, pH 7.4) were obtained from Aldrich. BBL^TM Mueller Hinton broth used as microbial growth media was purchased from Becton, Dickinson and Company (Madrid, Spain). Sheep blood (5%) and Columbia Agar plates were acquired from BioMérieux (Madrid, Spain). American Type Culture Collection (ATCC) Gram-positive Staphylococcus aureus (S. aureus, ATCC 29213) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa, ATCC 27853) bacteria were obtained from Oxoid^TM (Wesel, Germany).