Axioimager m2 widefield

Manufactured by Zeiss

The AxioImager M2 is a widefield microscope system designed for versatile imaging applications. It features a high-performance optical system and advanced illumination technology to provide clear, high-quality images. The system is capable of a range of observation techniques, including brightfield, darkfield, and phase contrast microscopy.

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Lab products found in correlation

Zen pro, by Zeiss (1 mentions) Cm1900 cryostat, by Leica (1 mentions) Gene frame, by Thermo Fisher Scientific (1 mentions) Lsm exciter confocal microscope, by Zeiss (1 mentions) Hxp 120, by Zeiss (1 mentions) Plan apo 1.4 na oil immersion objective, by Zeiss (1 mentions)

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M2 AxioImager

3 protocols using axioimager m2 widefield

Immunofluorescence Analysis of Adoptive B Cell Transfer

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Spleens were first fixed in 4% PFA for 3 hours at room temperature, then
sequentially immersed in 18% and 30% sucrose, and finally embedded in OCT
(Fisher) and flash-frozen using dry ice/EtOH bath. Five-to-seven-micron sections
were cut using a Leica CM1900 cryostat and stored at −80C prior to
staining. Sections were blocked with 5% rat serum for 1 hour at RT, and then
stained for 1 hour at RT with FITC B220 (clone RA3–6B2), APC anti-CD3
(clone 17A2), and either biotin-conjugated anti-λ1 light chain (clone
R11–153), or PE anti-IgDa (clone REA484) to identify adoptively
transferred IgHEL Tg B cells. Images were captured with a Zeiss Axio Imager M2
widefield fluorescence microscope. Images were processed using Zen Pro (Zeiss)
and Adobe Photoshop (Adobe).

Tan C., Mueller J.L., Noviski M., Huizar J., Lau D., Dubinin A., Molofsky A., Wilson P.C, & Zikherman J. (2019). “Nur77 links chronic antigen stimulation to B cell tolerance by restricting the survival of self-reactive B cells in the periphery”. Journal of immunology (Baltimore, Md. : 1950), 202(10), 2907-2923.

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Fluorescence Microscopy Sample Preparation

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Samples for fluorescence microscopy were either immobilized onto agar pads constructed using Gene Frames (ThermoFisher), or applied directly to glass slides and imaged using a Zeiss AxioImager M2 widefield or Zeiss LSM Exciter confocal microscope, respectively. For FRAP experiments, samples were placed in a capillary on a glass slide and regions of interest were bleached using the Zen software bleaching mode on the Zeiss LSM Exciter confocal microscope. Further details on sample preparation, imaging conditions, and image analysis are provided in SI Appendix, Materials and Methods.

Tarafder A.K., von Kügelgen A., Mellul A.J., Schulze U., Aarts D.G, & Bharat T.A. (2020). Phage liquid crystalline droplets form occlusive sheaths that encapsulate and protect infectious rod-shaped bacteria. Proceedings of the National Academy of Sciences of the United States of America, 117(9), 4724-4731.

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Multicolor Fluorescence Imaging of Fixed Samples

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Images of fixed samples were acquired on a Zeiss AxioImager M2 widefield fluorescence microscope equipped with 63× PLAN APO (1.4 NA) oil-immersion objectives (Zeiss) and an HXP 120 metal-halide lamp used for excitation. Fluorescent probes were detected using the following filters: DAPI (excitation filter: 350/50 nm, dichroic mirror: 400 nm, emission filter: 460/50 nm), GFP (excitation filter: 470/40 nm, dichroic mirror: 495 nm, emission filter: 525/50 nm), Alexa 555 (excitation filter: 545/25 nm, dichroic mirror: 565 nm, emission filter: 605/70 nm), Alexa 647 (excitation filter: 640/30 nm, dichroic mirror: 660 nm, emission filter: 690/50 nm). Images were recorded using ZEN 2012 (Blue edition, Version 1.1.0.0) software and analyzed in ImageJ (1.48v) (75 (link)).

Rashid F.Z., Mahlandt E., van der Vaart M., Boer D.E., Varela Alvarez M., Henneman B., Brocken D.J., Voskamp P., Blok A.J., Shimizu T.S., Meijer A.H., Luijsterburg M.S., Goedhart J., Crémazy F.G, & Dame R.T. (2021). HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein. Nucleic Acids Research, 50(2), e10.

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