7500 sequence detector
The 7500 Sequence Detector is a real-time PCR instrument designed for gene expression analysis, SNP genotyping, and other genetic applications. It features a 96-well sample block and uses fluorescence detection to monitor the amplification of DNA samples during the PCR process.
Lab products found in correlation
45 protocols using 7500 sequence detector
Quantification of Lactobacillus in Fecal Samples
Quantitative Gene Expression Analysis
Quantitative gene expression analysis
RNA Isolation and Real-Time PCR Analysis
Gene Expression Analysis of Bacterial Cultures
were cultured in TSB overnight. The overnight cultures were diluted
(1:100) in fresh TSB or TSB with 10% spent culture of S. epidermidis and grown for 8 h at 37 °C with
shaking. The pellets were collected by centrifuge at 4000g for 10 min and broken by FastPrep-24 (MP 116005500). After centrifugation,
the supernatant was used to isolate the total RNA and further synthesize
complementary DNA according to the manufacturer’s instructions
(Qiagen). The cDNA was used as a template for real-time PCR using
SYBR-green PCR reagents (Roche) with primers listed in
Optical 96-well reaction plate using a 7500 Sequence Detector (Applied
Biosystems). All qRT-PCR experiments were performed in triplicate
using gyrB as an internal control gene at the mRNA
level.
Quantitative Real-Time PCR for Gene Expression
Extraction and Quantification of S. epidermidis RNA
Quantitative Real-Time PCR for Gene Expression
Real-time PCR reactions were carried out in a two-tube system and in singleplex. The real-time amplifications included 10 min at 95°C (AmpliTaq Gold activation), followed by 40 cycles at 95°C for 15 s and at 60°C for 1 min. Thermocycling and signal detection were performed with 7500 Sequence Detector (Applied Biosystems). Signals were detected according to the manufacturer’s instructions and the relative expression levels were calculated as it has been previously described (9 (link)).
Multiplex qMSP Analysis of Gene Promoter Methylation
Biofilm Formation Genes in S. aureus
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