Log In Sign up
The largest database of trusted experimental protocols

Maxwell 16 ffpe plus lev dna purification kit

Manufactured by Promega
Visit Supplier
Sourced in United States, Germany, France, United Kingdom

The Maxwell 16 FFPE Plus LEV DNA Purification Kit is a laboratory equipment product designed to extract and purify DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. The kit utilizes magnetic bead-based technology to isolate DNA, which can then be used for various downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Maxwell 16 blood dna purification kit, by Promega (8 mentions) Qubit dsdna hs assay kit, by Thermo Fisher Scientific (6 mentions) Maxwell 16 tissue dna purification kit, by Promega (5 mentions) Qubit dsdna br assay kit, by Thermo Fisher Scientific (5 mentions) Fluostar omega microplate reader, by BMG Labtech (5 mentions) Qubit 2.0 fluorometer, by Thermo Fisher Scientific (4 mentions) Picogreen fluorescence assay, by Thermo Fisher Scientific (4 mentions) Qiaamp circulating nucleic acid kit, by Qiagen (3 mentions) Qubit fluorometer, by Thermo Fisher Scientific (2 mentions) Sequencing adaptor, by Illumina (2 mentions) Quantus fluorometer, by Promega (2 mentions) Quantifluor one dsdna system, by Promega (2 mentions) Rneasy ffpe kit, by Qiagen (2 mentions) Nextseq 500 instrument, by Illumina (2 mentions) Maxwell system, by Promega (2 mentions) Maxwell 16 lev rna ffpe kit, by Promega (2 mentions) Maxwell 16 instrument, by Promega (2 mentions) Lightcycler taqman master, by Roche (2 mentions) Maxwell rsc blood dna kit, by Promega (2 mentions) Gotaq long pcr master mix, by Promega (2 mentions)

Spelling variants of this product

Maxwell 16 (79 citations) Maxwell kits (2 citations)

91 protocols using maxwell 16 ffpe plus lev dna purification kit

1

Gastric Cancer Metastasis Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
This study was conducted in compliance with the Helsinki Declaration. The institutional review board at Stanford University School of Medicine approved the study protocol (19071). We obtained a matched set of samples including a gastric primary cancer, two metastases from each ovary, and normal stomach tissue (Additional file 2: Figure S2). These samples were obtained from the Stanford Cancer Institute tissue bank. Based on a formal pathology review, the overall tumor purity of these samples was estimated at less than 40%. Macro-dissection of the tumor samples was performed to increase the tumor DNA fraction in the final extraction. We used the Maxwell 16 FFPE Plus LEV DNA purification kit to extract genomic DNA from the formalin-fixed paraffin-embedded (FFPE) samples and Maxwell 16 Tissue DNA purification to extract DNA from frozen samples according to the manufacturer’s protocol (Promega, Madison, WI, USA). Final DNA concentrations were quantified with the Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA).
Greer S.U., Nadauld L.D., Lau B.T., Chen J., Wood-Bouwens C., Ford J.M., Kuo C.J, & Ji H.P. (2017). Linked read sequencing resolves complex genomic rearrangements in gastric cancer metastases. Genome Medicine, 9, 57.
+ Open protocol
+ Expand
2

Nucleic Acid Extraction from FFPE and Blood Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was extracted from FFPE tissues using Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega) and blood samples from QiaAmp Mini Kit (QIagen) following manufacturers’ instructions. DNA concentration was dosed by Qubit fluorometer (Invitrogen), absorbances with NanoDrop spectrophotometer (Thermo Fisher Scientific), and quality profiles assessed on Tapestation (Agilent Technologies).
Total RNA from FFPE tissues was extracted using FormaPure RNA (Beckman Coulter). RNA concentration and absorbances were analyzed with NanoDrop spectrophotometer (Thermo Fisher Scientific), and quality profiles assessed on Tapestation (Agilent Technologies).
Rousseau B., Bieche I., Pasmant E., Hamzaoui N., Leulliot N., Michon L., de Reynies A., Attignon V., Foote M.B., Masliah-Planchon J., Svrcek M., Cohen R., Simmet V., Augereau P., Malka D., Hollebecque A., Pouessel D., Gomez-Roca C., Guimbaud R., Bruyas A., Guillet M., Grob J.J., Duluc M., Cousin S., de la Fouchardiere C., Flechon A., Rolland F., Hiret S., Saada-Bouzid E., Bouche O., Andre T., Pannier D., El Hajbi F., Oudard S., Tournigand C., Soria J.C., Champiat S., Gerber D.G., Stephens D., Lamendola-Essel M.F., Maron S.B., Diplas B.H., Argiles G., Krishnan A.R., Tabone-Eglinger S., Ferrari A., Segal N.H., Cercek A., Hoog-Labouret N., Legrand F., Simon C., Lamrani-Ghaouti A., Diaz LA J.r., Saintigny P., Chevret S, & Marabelle A. (2022). PD-1 Blockade in Solid Tumors with Defects in Polymerase Epsilon. Cancer discovery, 12(6), 1435-1448.
+ Open protocol
+ Expand
3

Automated DNA and RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA and RNA were extracted using the Promega Maxwell RSC device (Promega, Madison, USA). DNA was isolated with the Maxwell 16 Tissue DNA purification kit, the Maxwell 16 blood purification kit, or the Maxwell 16 FFPE Plus LEV DNA purification kit (Promega, Madison, USA) according to the manufacturer’s instructions. RNA was extracted with the Maxwell 16 LEV simplyRNA or the Maxwell 16 LEV RNA FFPE purification kit (Promega, Madison, USA) following the manufacturer’s protocols. Agilent 4200 TapeStation (Agilent Technologies, Santa Clara, USA) was used to determine RNA integrity numbers.
Kaulen L.D., Denisova E., Hinz F., Hai L., Friedel D., Henegariu O., Hoffmann D.C., Ito J., Kourtesakis A., Lehnert P., Doubrovinskaia S., Karschnia P., von Baumgarten L., Kessler T., Baehring J.M., Brors B., Sahm F, & Wick W. (2023). Integrated genetic analyses of immunodeficiency-associated Epstein-Barr virus- (EBV) positive primary CNS lymphomas. Acta Neuropathologica, 146(3), 499-514.
+ Open protocol
+ Expand
4

Microdissection of EATL-II Tumour

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fifteen cases of routinely processed (FFPE) surgical specimens of EATL-II were selected for availability of tumour and matching non-tumoural intestinal tissue. Areas with highest tumour cell content and those devoid of neoplastic cells were marked on HE slides by a pathologist and subsequently reported on corresponding FFPE sections stained with toluidine blue. Manual microdissection of the regions of interest was performed under a microscope, followed by genomic DNA extraction (Maxwell 16 FFPE Plus LEV DNA Purification Kit, Promega).
Roberti A., Dobay M.P., Bisig B., Vallois D., Boéchat C., Lanitis E., Bouchindhomme B., Parrens M.C., Bossard C., Quintanilla-Martinez L., Missiaglia E., Gaulard P, & de Leval L. (2016). Type II enteropathy-associated T-cell lymphoma features a unique genomic profile with highly recurrent SETD2 alterations. Nature Communications, 7, 12602.
+ Open protocol
+ Expand
5

Targeted Sequencing of Cancer Genes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was extracted from 40 μg of FFPE tumor tissue using a Maxwell 16 FFPE Plus LEV DNA Purification kit (Promega, Madison, WI, USA). Samples of 50–200 ng of extracted DNA were sheared to ~100–400 bp by sonication and were then subjected to end-repair, dA-addition, and ligation of indexed Illumina (San Diego, CA, USA) sequencing adaptors [11 (link)]. Sequencing libraries were hybridization-captured using a pool of >24 000 individually synthesized 5′-biotinylated DNA oligonucleotides (Integrated DNA Technologies, Coralville, IA, USA). These baits were designed to target ~1.5 MB of the human genome, including 4557 exons of 287 cancer-related genes, 47 introns of 19 genes frequently rearranged in cancer, and 3549 polymorphisms located throughout the genome. DNA sequencing was performed using the HiSeq instrument (Illumina) with 49 × 49 paired-end reads, targeting >500× unique median sequence coverage. Sequence data analysis and quality control measures are described in the Supplementary material, along with gene expression data analysis, support vector machine training with sequence and protein structural features, and ATM protein modeling.
Plimack E.R., Dunbrack R.L., Brennan T.A., Andrake M.D., Zhou Y., Serebriiskii I.G., Slifker M., Alpaugh K., Dulaimi E., Palma N., Hoffman-Censits J., Bilusic M., Wong Y.N., Kutikov A., Viterbo R., Greenberg R.E., Chen D.Y., Lallas C.D., Trabulsi E.J., Yelensky R., McConkey D.J., Miller V.A., Golemis E.A, & Ross E.A. (2015). Defects in DNA Repair Genes Predict Response to Neoadjuvant Cisplatin-based Chemotherapy in Muscle-invasive Bladder Cancer. European urology, 68(6), 959-967.
+ Open protocol
+ Expand
6

POFUT1 Copy Number Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was extracted from normal and tumor tissues with Maxwell® 16 FFPE Plus LEV DNA Purification Kit and Maxwell® 16 IVD device (Promega, Madison, WI, USA) according to the manufacturer’s protocol. Genomic DNA concentration was determined using Quantifluor® ONE dsDNA system (Promega) and measured with Quantus™ Fluorometer (Promega) following manufacturer’s recommendations. Taqman™ copy number assay for POFUT1 (Hs02487189_cn) and RNAse P reference assays (4403326) were used with Gene Expression Master Mix (Applied Biosystems™, Thermo Fisher Scientific, Waltham, MA, USA), according to product literature. Twenty nanograms of gDNA were run in triplicate on QuantStudio 3 real-time PCR system (Applied Biosystem™). POFUT1 copy number was estimated using ∆∆Ct method [65 (link)].
Chabanais J., Labrousse F., Chaunavel A., Germot A, & Maftah A. (2018). POFUT1 as a Promising Novel Biomarker of Colorectal Cancer. Cancers, 10(11), 411.
+ Open protocol
+ Expand
7

Tissue Extraction and Microarray Construction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Additional hematoxylin and eosin stained sections of normal and tumor were prepared from the selected FFPE blocks and annotated by a GI pathologist (PK) for DNA/RNA extraction and TMA construction. For both DNA and RNA extractions, 6 × 5 micron blank sections were cut from each block and dewaxed in xylene and alcohol. Under direct visualization using magnifying glass annotated areas were scrapped off using a scalpel blade into 1.5 ml tubes. Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, UK) was used for DNA extraction and RNeasy FFPE Kit (Qiagen, UK) for RNA extraction. Elution was in a volume of 50 ul. TMAs were constructed using 1 mm cores from tumors in triplicate and normals in duplicate on a Beecher MTA1 (Beecher Instruments Inc., WI), following international standards [25 (link)].
Alvi M.A., McArt D.G., Kelly P., Fuchs M.A., Alderdice M., McCabe C.M., Bingham V., McGready C., Tripathi S., Emmert-Streib F., Loughrey M.B., McQuaid S., Maxwell P., Hamilton P.W., Turkington R., James J.A., Wilson R.H, & Salto-Tellez M. (2015). Comprehensive molecular pathology analysis of small bowel adenocarcinoma reveals novel targets with potential for clinical utility. Oncotarget, 6(25), 20863-20874.
+ Open protocol
+ Expand
8

FFPE Whole-Exome Sequencing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole‐exome sequencing was performed on DNA extracted from formalin‐fixed paraffin‐embedded (FFPE) tumor samples (Maxwell® 16 FFPEPlus LEV DNA Purification Kit, Promega, Madison, WI, USA) in addition to freshly obtained peripheral blood or normal tissue. WES Libraries were prepared according to manufacturer’s protocol (SureSelect XT Human All Exon v5, Agilent, Santa Clara, CA, USA). Finally, libraries were sequenced in a HiSeq2500 (Illumina, San Diego, CA, USA), 2X100 Paired‐end. Reads were aligned against the hg19 reference genome.
Frigola J., Navarro A., Carbonell C., Callejo A., Iranzo P., Cedrés S., Martinez‐Marti A., Pardo N., Saoudi‐Gonzalez N., Martinez D., Jimenez J., Sansano I., Mancuso F.M., Nuciforo P., Montuenga L.M., Sánchez‐Cespedes M., Prat A., Vivancos A., Felip E, & Amat R. (2021). Molecular profiling of long‐term responders to immune checkpoint inhibitors in advanced non‐small cell lung cancer. Molecular Oncology, 15(4), 887-900.
+ Open protocol
+ Expand
9

PCR-based HPV Genotyping Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HPV genotyping was performed as previously described [10 (link)]. In short, DNA was extracted from tumour-containing paraffin slides using the automated Maxwell® 16 FFPE Plus LEV DNA Purification Kit (Promega, Madison, USA) and amplified with biotinylated primers; PCR products were incubated with oligonucleotide-precoated strips using the HPV typing kit from AID diagnostics (Strassberg, Germany) according to the manufacturer’s protocol (Figure 1). The kit detects 15 different HPV genotypes (6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58 and 59).

Representative results of HPV genotyping using a PCR-based approach. First lane shows a HPV-16-positive case, while in lane 2, no HPV DNA was detectable. Lane 3 shows detection of LR-HPV DNA that was later identified as HPV isoform 6. LR-HPV, low-risk human papillomavirus.

Steinestel J., Al Ghazal A., Arndt A., Schnoeller T.J., Schrader A.J., Moeller P, & Steinestel K. (2015). The role of histologic subtype, p16INK4a expression, and presence of human papillomavirus DNA in penile squamous cell carcinoma. BMC Cancer, 15, 220.
+ Open protocol
+ Expand
10

Archival Tumor Tissue DNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was isolated from archival tumor tissue using the Maxwell 16 FFPE Plus LEV DNA purification kit (Promega, Madison, WI, USA). DNA from whole blood (germline DNA) was isolated using the Maxwell 16 Blood DNA Purification kit (Promega) following the manufacturer’s instructions. The quantity of extracted genomic DNA was assessed by a fluorometric method with a Qubit device.
Galland L., Ballot E., Mananet H., Boidot R., Lecuelle J., Albuisson J., Arnould L., Desmoulins I., Mayeur D., Kaderbhai C., Ilie S., Hennequin A., Bergeron A., Derangère V., Ghiringhelli F., Truntzer C, & Ladoire S. (2022). Efficacy of platinum-based chemotherapy in metastatic breast cancer and HRD biomarkers: utility of exome sequencing. NPJ Breast Cancer, 8, 28.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!