Human umbilical vein endothelial cells (HUVECs) and normal human lung fibroblasts (NHLF) were purchased from Lonza (Walkersville, MD) and used before passage 6 and passage 8 respectively. HUVECs were cultured in EGM-2 media containing 2% FBS and supplements such as hEGF, hydrocortisone, VEGF, hFGF-B, R3-IGF-1, ascorbic acid, heparin, and gentamicin/amphotericin-B (Lonza, Walkersville, MD) [44 (link)]. NHLFs were cultured in FGM-2 media containing 2% FBS and supplements such as hFGF-B, insulin, and gentamicin/amphotericin-B (Lonza, Walkersville, MD) [45 (link)]. U87-MG cells, a gift from Professor Nathan D. Price (ISB, Seattle), were cultured in DMEM with 10% FBS and 1% penicillin/streptomycin [29 (link)]. All cell types were cultured at 37 °C in 5% CO 2.
Hfgf b
Manufactured by Lonza
Sourced in Switzerland, United States, Belgium
HFGF-B is a laboratory equipment product manufactured by Lonza. It is designed for high-throughput cell culture applications, providing a consistent and controlled environment for cell growth and proliferation. The core function of HFGF-B is to facilitate the efficient culture of a variety of cell types, supporting their optimal growth and maintenance in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Human epidermal growth factor (hegf), by Lonza (32 mentions)
Vascular endothelial growth factor (vegf), by Lonza (24 mentions)
Ascorbic acid, by Lonza (20 mentions)
R3 igf 1, by Lonza (18 mentions)
Ga 1000, by Lonza (17 mentions)
Heparin, by Lonza (14 mentions)
Hydrocortisone, by Lonza (14 mentions)
Fetal bovine serum (fbs), by Lonza (12 mentions)
Egm 2, by Lonza (5 mentions)
Huvecs, by Lonza (4 mentions)
Insulin, by Lonza (4 mentions)
R igf 1, by Lonza (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Trypsin neutralizing solution, by Lonza (3 mentions)
Ebm 2 medium, by Lonza (3 mentions)
Gentamicin amphotericin b, by Lonza (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Hmvec, by Lonza (2 mentions)
Smgm 2 smooth muscle growth medium 2, by Lonza (2 mentions)
Human umbilical vein endothelial cells (huvec), by Lonza (2 mentions)
38 protocols using hfgf b
1
Culture and Maintenance of HUVEC, NHLF, and U87-MG Cells
2
Cell Culture Protocol for Lung Cancer Research
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The human cerebral microvascular endothelial cell line (hCMEC/D3) was donated by Professor Pierre Olivier Couraud (Institute of Cochin, INSERM, Paris, France).36 hCMEC/D3 cells were cultured in endothelial basal medium-2 (EBM-2) supplemented vascular endothelial growth factor (VEGF), human epidermal growth factor (hEGF), R3-insulin-like growth factor-1 (R3-IGF-1), ascorbic acid, hydrocortisone, human fibroblast growth factor-beta (hFGF-β), heparin (all from Lonza), and 2% human serum (Biosera). Primary NSCLC cells (A549 and COR-L105) were purchased from Sigma and metastatic NSCLC cells from cervical lymph nodes (NCI-H1299) from ATCC. Low-passage brain-metastatic NSCLC cell lines (SEBTA-001 and SEBTA-005) were established in house using biopsies from patients with lung-brain secondary tumors. All NSCLCs were cultured in Dulbecco′s modified Eagle medium supplemented with 2% human serum (Biosera) and maintained in 5% CO2 and humidified atmosphere at 37°C. All cell lines were subjected to routine mycoplasma testing, utilizing a kit from Lonza. Cell authentication was conducted using a microfluidic electrophoresis system incorporating an Agilent 2100 Bioanalyzer (Agilent Technologies\) to analyze STR-PCR fragments from 10 human genomic loci of human cell lines.37 (link)
3
Endothelial Cell Modulation by TGFβ2 and N-FGFR1
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The HMVECs were cultured in EBM-2 medium supplemented with EGM-2 (5.5 mmol/l glucose, fetal bovine serum, hydrocortisone, hFGF-β, VEGF, R-IGF-1, ascorbic acid, hEGF, GA-1000 and heparin) (Lonza, Alpharetta, GA, USA). When the cells reached 70–80% confluence, TGFβ2 (5 ng/ml), N-FGFR1 (1.5 μg/ml) and TGFβ2 with either the N-FGFR1 or the neutralizing TGFβ (1, 2, 3) antibody (1.0 μg/ml) was added to the experimental medium (a mixture of HuMedia-MVG in serum-free RPMI 1640 medium at a 1 : 3 ratio) with or without preincubation with 100 nM AcSDKP for 2 h.
4
Culturing BAE and HAEC Cells
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BAE cells were cultured in Dulbecco's Modified Eagle's Medium-AQ (DMEM-AQ, Sigma Aldrich) supplemented with 10% heat inactivated fetal bovine serum (FBS; Sigma Aldrich), and 1% penicillin/streptomycin mixture. HAEC's were cultured in EBM-2 Basal Medium supplemented with human epidermal growth factor (hEGF), vascular endothelial growth factor (VEGF), R3-insulin-like growth factor-1 (R3-IGF-1), ascorbic acid, hydrocortisone, human fibroblast growth factor-beta (hFGF-β), FBS, and gentamicin/amphotericin-B (GA) (Lonza). Cells were maintained at 37°C in a humidified 5% CO2 incubator. BAE cells and HAEC were used between passages 5–9.
5
Endothelial Cell Modulation and Kidney Cell Response
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The human dermal microvascular endothelial cells (HMVECs) were cultured in EBM-2 medium supplemented with EGM-2 which contained fetal bovine serum, 5.5 mmol/L-glucose, hydrocortisone, VEGF, R-IGF-1, hFGF-β, ascorbic acid, GA-1000, hEGF, and heparin (Lonza, Alpharetta, GA, USA). When the cells reached 70-80% confluence, TGFβ2 (5 ng/mL), N-FGFR1 (1.5 μg/mL), apelin (100 nM), or ML221 (10 μM) was added to the experimental medium (a mixture of HuMedia-MVG in serum-free RPMI 1640 medium at a 1 : 3 ratio).
Human HK-2 cells were cultured in MEM supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA). To establish the conditioned medium experiment [8 (link)], HMVECs were transfected with scramble or apelin siRNA for 6 h, after which the medium was replaced with fresh EBM-2 medium. After incubation with fresh medium for 48 h, the experimental medium of HMVECs was harvested and transferred to cultured HK2 cells.
Human HK-2 cells were cultured in MEM supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA). To establish the conditioned medium experiment [8 (link)], HMVECs were transfected with scramble or apelin siRNA for 6 h, after which the medium was replaced with fresh EBM-2 medium. After incubation with fresh medium for 48 h, the experimental medium of HMVECs was harvested and transferred to cultured HK2 cells.
6
Endothelial Cell Adhesion Assay
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Human dermal microvascular endothelial cells (HMVECs, Lonza, Basel, Switzerland), maintained in EBM-2 medium supplemented with EGM-2 (glucose concentration 5.5 mmol/l, containing fetal bovine serum, hydrocortisone, hFGF-β, VEGF, R-IGF-1, ascorbic acid, hEGF, GA-1000 and heparin, Lonza, Alpharetta, GA), were used in this experiment. When the HMVECs on the adhesion reagent (Kurabo Medical, Osaka, Japan) reached 70% confluence, the media were replaced with the experimental media (HuMedia-MVG in serum-free RPMI-1640 at a 1:3 ratio) with or without mAb clone 9EG7 (5 μg/ml) for 1 h. In the control well, control mAb immunoglobulin G was added. Forty-eight hours later, protein lysate was harvested for western blot analysis.
7
Culturing Lung and Endothelial Cells
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Human lung adenocarcinoma cells line A549, human umbilical vein
endothelial cell fusion cell line Eahy926, were purchased from ATCC
(American Type Culture Collection, VA, USA). These cells were cultured
in DMEM medium supplemented with 10% fetal calf serum plus 1%
ampicillin in a humidified atmosphere with 5% CO2 at 37 °C. Primary
human umbilical vein endothelial cell (HUVEC) isolated sterile from
umbilical cord of newborn after delivery, was cultured in endothelial
cell growth medium-2 (EGM-2, purchased from LONZA, art no: cc-3162)
including 0.1% hEGF, 0.04% hydrocortisone, 0.1% CA-1000, 2% FBS, 0.4%
hFGF-B, 0.1% VEGF, 0.1% R3-IGF-1, 0.1% heparin and 0.1% ascorbic acid
(Lonza, Basel, Switzerland).
endothelial cell fusion cell line Eahy926, were purchased from ATCC
(American Type Culture Collection, VA, USA). These cells were cultured
in DMEM medium supplemented with 10% fetal calf serum plus 1%
ampicillin in a humidified atmosphere with 5% CO2 at 37 °C. Primary
human umbilical vein endothelial cell (HUVEC) isolated sterile from
umbilical cord of newborn after delivery, was cultured in endothelial
cell growth medium-2 (EGM-2, purchased from LONZA, art no: cc-3162)
including 0.1% hEGF, 0.04% hydrocortisone, 0.1% CA-1000, 2% FBS, 0.4%
hFGF-B, 0.1% VEGF, 0.1% R3-IGF-1, 0.1% heparin and 0.1% ascorbic acid
(Lonza, Basel, Switzerland).
8
Characterization of UT-TERT and GM-TERT Cell Lines
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The human myometrial (UT-TERT) and fibroid (GM-TERT) cell lines were a kind gift from Dr. John Risinger and have been characterized previously (Carney et al., 2002 (link)). UT-TERT cells were cultured and maintained in SmGM™-2 Smooth Muscle Growth Medium-2 containing 5% FBS, 0.1% insulin, 0.2% basic human fibroblast growth factor (hFGF-b), 0.1% GA-100, and 0.1% human epidermal growth factor (hEGF) (Lonza, Walkersville, MD).
9
Characterization of UT-TERT and GM-TERT Cell Lines
10
Smooth Muscle Cell Culture Protocol
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